Research Describing Biological Protein Interactions in Terms of Protein States and State Transitions THE LiveDIP DATABASE*

Biological protein-protein interactions differ from the more general class of physical interactions; in a biological interaction, both proteins must be in their proper states (e.g. covalently modified state, conformational state, cellular location state, etc.). Also in every biological interaction, one or both interacting molecules undergo a transition to a new state. This regulation of protein states through protein-protein interactions underlies many dynamic biological processes inside cells. Therefore, understanding biological interactions requires information on protein states. Toward this goal, DIP (the Database of Interacting Proteins) has been expanded to LiveDIP, which describes protein interactions by protein states and state transitions. This additional level of characterization permits a more complete picture of the protein-protei

Clinical implication of changes in serum adiponectin in patients with hepatogenic diabetes

Adiponectin is a protein hormone that modulates glucose metabolism and fatty acid oxidation. We explored the clinical implication of serum adiponectin in hepatogenic diabetes. Serum adiponectin levels were determined using enzyme-linked immunochemistry assay in 78 individuals including 19 hepatogenic diabetes, 20 type 2 diabetes (T2D), 20 chronic liver disease and 19 healthy controls. Cases and controls were matched by gender and body mass index (BMI). There is no difference in serum adiponectin levels among hepatogenic diabetic, T2D and healthy control groups. The levels of adiponectin are highest in chronic liver disease and lowest in T2D. Insulin levels are highest in hepatic diabetics and lowest in T2D. Hepatic diabetics have the lowest insulin sensitivity index (ISI). Serum adiponectin levels were negatively correlated with triglycerides and total cholesterol in T2D. Serum adiponectin is significantly increased in chronic liver disease, but lacks association with hepatogenic diabetes.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000338422600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Multidisciplinary SciencesSCI(E)[email protected]

Modification of montmorillonite with poly(oxypropylene) amine hydrochlorides : Basal spacing, amount intercalated, and thermal stability

Few studies have explored the change in thermal stability of poly(oxypropylene) (POP) ammonium ions after intercalation, even though several studies have focused on the modification of montmorillonite (Mt) with POP amine hydrochloride. The purpose of the present study was to understand the effect of chain length of POP amine hydrochlorides on the basal spacing of modified Mt, and the amount and thermal stability of the ammonium ions intercalated. The relations between basal spacing, organic fraction, and thermal stability of the ammonium ions intercalated were also explored. Series of modified Mt were prepared via ion-exchange between Na-montmorillonite (Na -Mt) and POP diammonium ions or POP triammonium ions with different chain lengths, and were then characterized by Fourier-transform infrared spectroscopy, X-ray diffraction, and simultaneous differential scanning calorimetry-thermogravimetric analysis. The results revealed that the basal spacing of modified Mt increased with the hydrophobic chain length of the POP ammonium ions. The amount of triammonium ions intercalated was close to the theoretical amount, while the organic fraction of modified Mt was directly proportionalto the basalspacing of modified Mt. The intercalated ammonium ions were, therefore, contained within the interlayer space of Mt. After intercalation, the thermal stability of the POP ammonium ions with various chain lengths was reduced; i.e. T was reduced by 7-60°C for short-chain POP ammonium ions (D400 and T403) and by 177-192°C for long-chain ions (D2000, D4000, T3000, and T5000)

Assimilation of Sentinel-1 Derived Sea Surface Winds for Typhoon Forecasting

High-resolution synthetic aperture radar (SAR) wind observations provide fine structural information for tropical cycles and could be assimilated into numerical weather prediction (NWP) models. However, in the conventional method assimilating the u and v components for SAR wind observations (SAR_uv), the wind direction is not a state vector and its observational error is not considered during the assimilation calculation. In this paper, an improved method for wind observation directly assimilates the SAR wind observations in the form of speed and direction (SAR_sd). This method was implemented to assimilate the sea surface wind retrieved from Sentinel-1 synthetic aperture radar (SAR) in the basic three-dimensional variational system for the Weather Research and Forecasting Model (WRF 3DVAR). Furthermore, a new quality control scheme for wind observations is also presented. Typhoon Lionrock in August 2016 is chosen as a case study to investigate and compare both assimilation methods. The experimental results show that the SAR wind observations can increase the number of the effective observations in the area of a typhoon and have a positive impact on the assimilation analysis. The numerical forecast results for this case show better results for the SAR_sd method than for the SAR_uv method. The SAR_sd method looks very promising for winds assimilation under typhoon conditions, but more cases need to be considered to draw final conclusions

Dietary Supplementation of Ancientino Ameliorates Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation and Oxidative Stress

Ancientino, a complex dietary fiber supplement mimicking the ancient diet, has improved chronic heart failure, kidney function, and constipation. However, its effect on ulcerative colitis is unknown. This study explores the impact of Ancientino on colitis caused by dextran sulfate sodium (DSS) and its mechanisms. Data analyses showed that Ancientino alleviated bodyweight loss, colon shortening and injury, and disease activity index (DAI) score, regulated levels of inflammatory factors (tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), interleukin-1 beta (IL-1β), and interleukin 6 (IL-6)), reduced intestinal permeability (d-lactate and endotoxin), fluorescein isothiocyanate–dextran (FITC-dextran), and diamine oxidase (DAO), repaired colonic function (ZO-1 and occludin), and suppressed oxidative stress (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA)) in vivo and in vitro. In short, this study demonstrated that Ancientino alleviates colitis and exerts an anticolitis effect by reducing inflammatory response, suppressing oxidative stress, and repairing intestinal barrier function. Thus, Ancientino may be an effective therapeutic dietary resource for ulcerative colitis