Additional file 1 of Aptamer-based protein molecule detection via cyclic reverse transcription coupling with self-priming hairpin-triggered CRISPR-Cas12a system

Additional file 1: Table S1. Nucleic acid sequence used in the experiments

Nitrogen-Doped Mesoporous Carbons for Supercapacitor Electrodes with High Specific Volumetric Capacitance

To pursue the miniaturization of supercapacitors in practical use, it is critical to construct an efficient but limited porosity of a nanocarbon-based electrode for simultaneously obtaining a high utilization of energy storage places and high coating density. However, current studies dominantly focus on the enhancement of specific mass capacitance (<i>C</i>_m) by increasing the pore volume and surface area, leading to a low coating density and, thereby, resulting in a low specific volumetric capacitance (<i>C</i>_V). We report herein the fabrication of a nitrogen-doped mesoporous carbon (NNCM), whose tunable pore volume coupled with the fixed mesopore size offers us the possibility to control the coating density, thus optimizing the <i>C</i>_V and <i>C</i>_m for different application purposes. As a result, NNCM with the highest pore volume and surface area of 2.11 cm³ g^–1 and 663 m² g^–1 demonstrates the highest <i>C</i>_m (190 F g^–1) but lowest <i>C</i>_V (124 F cm^–3) because the overhigh porosity reduces the coating density greatly. NNCM with moderate pore volume and surface area of 1.22 cm³ g^–1 and 489 m² g^–1 shows the highest <i>C</i>_V of 200 F cm^–3, although it presents a low <i>C</i>_m of 147 F g^–1. These results may raise concerns about constructing a suitable porosity to realize a target-oriented use, particularly those targeting miniaturized devices

Ultra-Wide-Range Electrochemical Sensing Using Continuous Electrospun Carbon Nanofibers with High Densities of States

Carbon-based sensors for wide-range electrochemical detection of redox-active chemical and biological molecules were fabricated by the electrospinning of polyacrylonitrile fibers directly onto a polyacrylonitrile-coated substrate followed by carbonization at 1200 °C. The resulting electrospun carbon nanofibers (ECNFs) were firmly attached to the substrate with good mesh integrity and had high densities of electronic states (DOS), which was achieved without need for further modifications or the use of any additives. The mass of ECNFs deposited, and thus the electroactive surface area (ESA) of the sensor, was adjusted by varying the electrospinning deposition time, thereby enabling the systematic manipulation of the dynamic range of the sensor. A standard redox probe (Fe­(CN)₆^3–/4–) was used to demonstrate that the ECNF sensor exhibits strong electrocatalytic activity without current saturation at high analyte concentrations. Dopamine was used as a model analyte to evaluate the sensor performance; we find that the ECNF device exhibits a dynamic range ∼10⁵ greater than that of many existing carbon-based sensors. The ECNF sensors exhibited excellent sensitivity, selectivity, stability, and reproducibility for dopamine detection

Identification of the Nearby Hydroxyls’ Role in Promoting HCHO Oxidation over a Pt Catalyst

Insight into the relationship between catalytic trends and physicochemical properties of composite nanoparticles is essential for their rational design. Herein, a series of 3d-M (M = Mn, Fe, Co, Ni) metal hydroxide-promoted PtM­(OH)_<i>x</i>/Al₂O₃ catalysts are developed and well characterized for establishing the catalytic HCHO oxidation reactivity trend as a function of more fundamental properties, such as hydroxyl concentration and adsorption strength. The reactivity of PtM­(OH)_<i>x</i>/Al₂O₃ exhibits an increasing trend of Mn < Fe < Co < Ni, which is governed by their OH–M^2+δ bond strength (Ni < Co < Fe < Mn) and surface hydroxyl concentration (Mn < Fe < Co < Ni). Both PtCo­(OH)_<i>x</i>/Al₂O₃ and PtNi­(OH)_<i>x</i>/Al₂O₃ exhibit a (>)­95% HCHO conversion and (>)­100 h performance stability at 30 °C with a low 0.2 wt % Pt loading amount. The identification of these catalytic trends provides foundations for composite active sites design for HCHO oxidation and other hydroxyl-involved reactions

Table1_Case report: A case of new mutation in SERPINC1 leading to thrombotic microangiopathy.DOCX

Introduction: Hereditary antithrombin-III deficiency can significantly increase the risk for thrombosis, which is common in limb deep vein and pulmonary cases. However, thrombotic microangiopathy (TMA) caused by hereditary antithrombin deficiency is rare.Case Presentation: We reported the case of a 32-year-old Chinese female patient with TMA with renal injury caused by decreased antithrombin-III activity due to a new mutation (chr1-173884049 c.50A>G) in SERPINC1, which encodes antithrombin-III. In this case, the patient had no history of relevant drug use, diabetes, or monoclonal plasma cells in the bone marrow puncture. Consequently, TMA of the kidney was considered secondary to hereditary antithrombin-III deficiency. Gene detection was the only clue that led us to suspect that TMA was caused by hereditary antithrombin deficiency.Conclusion: Our findings indicated that for patients with repeated findings of antithrombin-III activity less than 50%, the possibility of antithrombin-III deficiency and complete gene detection must be considered immediately after excluding the use of anticoagulants and lack of availability to facilitate early detection, diagnosis, and intervention.</p

Three-Dimensional Macroporous Polypyrrole-Derived Graphene Electrode Prepared by the Hydrogen Bubble Dynamic Template for Supercapacitors and Metal-Free Catalysts

We report a general method for the fabrication of three-dimensional (3D) macroporous graphene/conducting polymer modified electrode and nitrogen-doped graphene modified electrode. This method involves three consecutive steps. First, the 3D macroporous graphene (3D MG) electrode was fabricated electrochemically by reducing graphene oxide dispersion on different conducting substrates and used hydrogen bubbles as the dynamic template. The morphology and pore size of 3D MG could be governed by the use of surfactants and the dynamics of bubble generation and departure. Second, 3D macroporous graphene/polypyrrole (MGPPy) composites were constructed via directly electropolymerizing pyrrole monomer onto the networks of 3D MG. Due to the benefit of the good conductivity of 3D MG and pseudocapacitance of PPy, the composites manifest outstanding area specific capacitance of 196 mF cm^–2 at a current density of 1 mA cm^–2. The symmetric supercapacitor device assembled by the composite materials had a good capacity property. Finally, the nitrogen-doped MGPPy (N-MGPPy or MGPPy-X) with 3D macroporous nanostructure and well-regulated nitrogen doping was prepared via thermal treatment of the composites. The resultant N-MGPPy electrode was explored as a good electrocatalyst for the oxygen reduction reaction (ORR) with the current density value of 5.56 mA cm^–2 (−0.132 V vs Ag/AgCl). Moreover, the fuel tolerance and durability under the electrochemical environment of the N-MGPPy catalyst were found to be superior to the Pt/C catalyst

Additional file 1: Figure S1. of Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells

showing identification of WJ-MSCs. (A) Observation of WJ-MSCs under a phase-contrast microscope. (a) Seven days after the tissues of Wharton’s jelly were plated, many triangular and spindle-shaped cells dissociated from the tissues. (b) About half a month later, these adherent cells were able to reach 80% confluence. (c) Third-generation cells exhibited a spindle shape and upon reaching confluence formed a whirlpool-like pattern. Bar represents 200 μm. (B) Surface antigens of WJ-MSCs in flow cytometry. WJ-MSCs were positive for CD90 and CD105; WJ-MSCs were negative for CD14, CD34, CD45, CD79a, and HLA-DR. Results confirmed that cells were MSCs but nonhematopoietic. (C) Differentiation potential of WJ-MSCs toward osteogenic and adipogenic lineages. Osteogenic differentiation assayed using the von Kossa procedure and adipogenic differentiation determined by formation of lipid vacuoles after induction. (a) No mineralized matrix formation found in WJ-MSCs cultured in regular growth medium. (b) Osteogenic differentiation determined by staining with Alizarin red after osteogeneic induction. (c) No lipid vacuoles found in WJ-MSCs cultured in regular medium. (d) Adipogenic differentiation detected by Oil red O staining. Bar represents 400 μ

Additional file 2: Figure S2. of Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells

showing identification of ESCs and EECs. (A) Morphological characteristics of ESCs. Bar represents 200 μm. (B) Morphological characteristics of EECs. Bar represents 200 μm. (C) Observation of ESCs after immunofluorescent staining. Results show ESCs in primary culture positively stained by vimentin and CD13 but negatively stained for cytokeratin and CD9. (a), (e) Nuclear counterstaining with Hoechst 33342. (b) ESCs positively stained by vimentin. (c) ESCs positively stained by CD13. (d) Merger of (a)–(c). (f) ESCs negatively stained by cytokeratin. (g) ESCs negatively stained by CD9. (h) Merger of (e)–(g). Bar represents 200 μm. (D) Observation of EECs after immunofluorescent staining. Results show that EECs in primary culture were positively stained by cytokeratin and CD9 but negatively stained for vimentin and CD13. (a), (e) Nucleal counterstaining with Hoechst 33342. (b) EECs negatively stained by vimentin. (c) EECs negatively stained by CD13. (d) Merger of (a)–(c). (f) EECs positively stained by cytokeratin. (g) ESCs positively stained by CD9. (h) Merger of (e)–(g). Bar represents 200 μm (TIFF 31403 kb

Kaplan-Meier survival curves for PCa patients with and without <i>ERG</i> rearrangement.

<p>The cancer-related survival rates were compared between patients with and without <i>ERG</i> rearrangement using the log-rank test.</p

Univariate and multivariate analysis of variables associated with survival in PCa patients.

<p>HR = hazard ratio; CI = confidence interval; PSA = prostate-specific antigen.</p>a<p>not included in multivariate analysis.</p