Quantitative identification and bioinformatics analysis of tumor tissue proteins assayed by iTRAQ.

<p><b>(A)</b> All 132 differentially accumulated proteins were classified into three groups: biological process, molecular function, and cellular component through GO analysis. <b>(B)</b> The numbers of lipid/glucose metabolism-related proteins were shown through GO analysis.</p

Quantitative identification and bioinformatics analysis of tumor tissue proteins assayed by iTRAQ.

<p><b>(A)</b> All 132 differentially accumulated proteins were classified into three groups: biological process, molecular function, and cellular component through GO analysis. <b>(B)</b> The numbers of lipid/glucose metabolism-related proteins were shown through GO analysis.</p

Hypothetic pathways of SBP1-mediated anti-cancer functions <i>in vivo</i>.

<p>SBP1-mediated anti-cancer effects may be through lipid/glucose metabolism. The possible functional regulation between SBP1 and related proteins is illustrated.</p

SBP1 induction suppressed tumor metastasis <i>in vivo</i>

<p><b>(A)</b> Representative images of lungs and hematoxylin and eosin (HE)-staining of lungs isolated from mice that received tail vein injection of HCT116-Act cells (Act) and HCT116-TetSBP1 cells (TetSBP1). Each group contains 5 mice. Arrows illustrates the visible nodules and metastatic nodules in lung. Scale bar = 200 μm. <b>(B)</b> SBP1 induction inhibits tumor metastasis <i>in vivo</i>. The numbers of pulmonary metastatic nodules were counted under microscope and analyzed with Student’s t-test. Results are shown as mean±SD. **P<0.01.</p

Identified differentially accumulated proteins related to lipid metabolism.

<p>Identified differentially accumulated proteins related to lipid metabolism.</p

Identified differentially accumulated proteins related to glucose metabolism.

<p>Identified differentially accumulated proteins related to glucose metabolism.</p

SBP1 induction in nude mice inhibited tumor growth of xenografts.

<p><b>(A)</b> SBP1 induced by doxycycline in HCT116-TetSBP1 cells were validated by immunoblot analysis. <b>(B)</b> Photographs illustrate representative tumors in xenografts with SBP1 induction (TetSBP1, right) compared to tumors without SBP1 induction (Act, left). 10 mice in total. <b>(C-D)</b> SBP1 induction results in a decline of tumor volume and weight. Results were analyzed with Student’s t-test and shown as mean±SD. **P<0.01.</p

Tumor inhibition of SBP1 was associated with multiple signaling pathways <i>in vivo</i>.

<p>Several typical markers of different signaling pathways were examined using iTRAQ proteomic analysis protein samples. These cancer related signaling pathways respond differently to SBP1 induction.</p

Additional file 8 of Transcriptomic analysis reveals that Bacillomycin D-C16 induces multiple pathways of disease resistance in cherry tomato

Additional file 8: Table S8. Table of transcription factors after Bacillomycin D-C16 treatment versus control at time 12 h

Additional file 4 of Transcriptomic analysis reveals that Bacillomycin D-C16 induces multiple pathways of disease resistance in cherry tomato

Additional file 4: Table S4. Table of GO enrichment analysis after Bacillomycin D-C16 treatment versus control at time 12 h