Additional file 3 of Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosis

Additional file 3: Supplementary Figure 2. Sirt6 regulates lipophagy by inhibiting Wnt1. (A–G) Analysis of LC3, Beclin1, LAMP1, adipophilin, PLIN2, and P62 by immunoblotting and quantitative analysis (n = 6). Data are expressed as mean ± SE (One way ANOVA showed that there was statistical significance among groups. * P < 0.05 vs the control group; #P < 0.05 vs the ox-LDL group; &P < 0.05 vs the Ac-LDL+salinomycin group; $P < 0.05 vs the ox-LDL+Ad-Sirt6 group [n = 6])

Additional file 2 of Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosis

Additional file 2: Supplementary Figure 1. siRNA ATG5 and siRNA ATG7 worked by western blot. (A, C) Expression of ATG5 and ATG7 treated with siRNA ATG5 and siRNA ATG7 were evaluated by western blot; (B, D) Quantitative analysis of ATG5 and ATG7 expression by western blot. Data are expressed as mean ± SE (Student’s t-test showed that there was statistical significance between Con group and siRNA ATG5 with siRNA ATG7 group. * P < 0.01 vs the control group [n = 6])

Additional file 1 of Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosis

Additional file 1

Additional file 4 of Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosis

Additional file 4: Supplementary Figure 3. The effect of Sirt6 on lipid droplets degradation was SNF2H dependent. (A) Co-localization of lipid droplets and lysosomes analyzed by LysoTracker Red and BODIPY 493/503 staining were detected by confocal microscopy in the presence or absence of SNF2H administration (n=6 separate experiments)

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-4

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>f ROS in males is shown on the y-axis (expressed as the ratio of male vs. female). In PBS-perfused kidneys, the elevation of superoxide anion in males was statistically significant. This statistical significance diminished when kidneys were flushed with heparin. Also the elevation of total ROS in males attenuated (the ratio of male vs. female became smaller). B: Real-time PCR confirmed significant under-expression of Sod3 in males compared with females (p < 0.01)

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-3

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>en genders. At pre-puberty and at late puberty/early adulthood, superoxide anion did not differ significantly between genders. Superoxide anion peaked at early puberty, more abruptly in males than in females (p = 0.05), and dropped to pre-puberty levels as late puberty/early adulthood approached

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-2

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>les had the tendency to produce more total ROS, but the gender difference was not statistically significant

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-5

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>diagram the number of genes with gender-dependent expression (p = 0.001) in the respective groups is depicted

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-1

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>ith strongest expression changes between genders, the microarray data were confirmed by real-time PCR in Mhm rats

Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty-0

<p><b>Copyright information:</b></p><p>Taken from "Faster rates of post-puberty kidney deterioration in males is correlated with elevated oxidative stress in males vs females at early puberty"</p><p>http://www.biomedcentral.com/1471-2164/8/221</p><p>BMC Genomics 2007;8():221-221.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1934371.</p><p></p>diagram the number of genes with gender-dependent expression (p = 0.001) in the respective groups is depicted