Denatured-State Conformation As Regulator of Amyloid Assembly Pathways?

Additional file 1: Figure S1. Disease incidence of ginger bacterial wilt. Figure S2. The rarefaction curve of samples. Table S1. Soil Physicochemical Data. Table S2. The top ten Phyla of samples. Dataset S1. Discriminative taxa analyzed by LEfSe in all samples

Characteristics of the included case-control studies on the MTHFR A1298C polymorphism in cervical lesions.

<p>Abbreviations: HWE: Hardy-Weinberg Equilibrium; NA, not available; NS, not significant; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion; ICC, invasive cervical cancer; SIL, squamous intra-epithelial lesion.</p

Funnel plot analysis on the detection of the publication bias for the A1298C polymorphism.

<p>(A) Meta-analysis in a random-effects model for AC+CC <i>vs</i>. AA (dominant model). (B) Meta-analysis in a random-effects model for AC <i>vs.</i> AA. (C) Meta-analysis in a random-effects model for CC vs. AA. Each point represents an individual study for the indicated association. LogOR, natural logarithm of OR. Perpendicular line denotes the mean effect size.</p

Forest plot describing the association between the A1298C polymorphism and the risk of cervical lesions.

<p>(A) Meta-analysis in a random-effects model for AC+CC <i>vs.</i> AA (dominant model). (B) Meta-analysis in a random-effects model for AC <i>vs.</i> AA. (C) Meta-analysis in a random-effects model for CC <i>vs.</i> AA. Each study is shown by the point estimate of the OR (the size of the square is proportional to the weight of each study) and 95% CI for the OR (extending lines).</p

Methylenetetrahydrofolate Reductase (MTHFR) Polymorphisms and Susceptibility for Cervical Lesions: A Meta-Analysis

<div><h3>Background</h3><p>The association between the methylenetetrahydrofolate reductase (MTHFR) C677T/A1298C polymorphisms and the susceptibility to cervical lesions was unclear. This study was designed to investigate their precise association using a large-scale meta-analysis.</p> <h3>Methods</h3><p>The previous 16 studies were identified by searching PubMed, Embase and CBM databases. The crude odds ratios and their corresponding 95% confidence intervals (CIs) were used to estimate the association between the MTHFR C677T/A1298C polymorphisms and the susceptibility to the cervical lesions. The subgroup analyses were made on the following: pathological history, geographic region, ethnicity, source of controls and source of DNA for genotyping.</p> <h3>Results</h3><p>Neither of the polymorphisms had a significant association with the susceptibility to the cervical lesions in all genetic models. Similar results were found in the subgroup analyses. No association was found between the MTHFR C677T polymorphism and the cervical lesions in the Asia or the America populations though a significant inverse association was found in the Europe population (additive model: <em>P</em> = 0.006, OR = 0.83, 95% CI = 0.72–0.95; CT <em>vs</em>. CC: <em>P</em> = 0.05, OR = 0.83, 95% CI = 0.69–1.00; TT <em>vs</em>. CC: <em>P</em> = 0.05, OR = 0.73, 95% CI = 0.53–1.00). Interestingly, women with the MTHFR A1298C polymorphisms had a marginally increased susceptibility to invasive cancer (ICC) when compared with no carriers but no statistically significant difference in the dominant model (<em>P</em> = 0.06, OR = 1.21, 95% CI = 0.99–1.49) and AC <em>vs</em>. AA (<em>P</em> = 0.09, OR = 1.21, 95% CI = 0.97–1.51).</p> <h3>Conclusions</h3><p>The MTHFR C677T and A1298C polymorphisms may not increase the susceptibility to cervical lesions. However, the meta-analysis reveals a negative association between the MTHFR C677T polymorphisms and the cervical lesions, especially in the European populations. The marginal association between the MTHFR A1298C polymorphisms and the susceptibility to cervical cancer requires a further study.</p> </div

Flow diagram of the study selection process.

<p>Flow diagram of the study selection process.</p

Funnel plot analysis on the detection of the publication bias for the C677T polymorphism.

<p>(A) Meta-analysis in a random-effects model for CT+TT <i>vs.</i> CC (dominant model). (B) Meta-analysis in a random-effects model for CT <i>vs.</i> CC. (C) Meta-analysis in a random-effects model for TT <i>vs</i>. CC. Each point represents an individual study for the indicated association. LogOR, natural logarithm of OR. Perpendicular line denotes the mean effect size.</p

Characteristics of the included case-control studies on the MTHFR C677T polymorphism in cervical lesions.

<p>Abbreviations: HWE, Hardy-Weinberg Equilibrium; NA, not available; NS, not significant; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion; ICC, invasive cervical cancer; SIL, squamous intra-epithelial lesion.</p

Forest plot describing the association between the C677T polymorphism and the risk of cervical lesions.

<p>(A) Meta-analysis in a random-effects model for CT+TT <i>vs.</i> CC (dominant model). (B) Meta-analysis in a random-effects model for CT <i>vs.</i> CC. (C) Meta-analysis in a random-effects model for TT <i>vs</i>. CC. Each study is shown by the point estimate of the OR (the size of the square is proportional to the weight of each study) and 95% CI for the OR (extending lines).</p

Pooled Analysis on Association between the MTHFR A1298C polymorphism and the cervical lesion risk.

<p>Dominant model: CC+AC <i>vs.</i> AA; Recessive model: CC <i>vs.</i> AC+AA; Additive model: C <i>vs.</i> A; R, Random-effects model; F, fixed-effects model; ICC, invasive cervical cancer; ICC: invasive cervical cancer; SIL, squamous intra-epithelial lesion.</p