Desarrollo de un programa para optimizar el grado de servicio de las centrales digitales de la Ciudad de Guayaquil para IETEL R-2

Presenta un programa para calcular el grado de servicios y obtener una mejor calidad de servicio de las centrales telefónicas. Hace una breve descripción de las centrales telefónicas analógicas y digitales y de la medición de tráfico y estudio de rutas. Planifica la adaptación de la central digital a un computador personal y por último presenta el programa con sus algoritmos y ejemplos.GuayaquilIngeniero en Electricidad Especialización Electrónic

Additional file 11: of iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

The raw data of firmness, rind colour, SSC, volatile compounds, suger content, ethylene production and expression analysis of genes. (XLSX 19 kb

Severe maternal morbidity^a for Black and White birthing people: Massachusetts, 1998–2018.

aSevere maternal mortality without blood transfusion (SMM20).</p

Global Identification of EVI1 Target Genes in Acute Myeloid Leukemia

<div><p>The ecotropic virus integration site 1 (EVI1) transcription factor is associated with human myeloid malignancy of poor prognosis and is overexpressed in 8–10% of adult AML and strikingly up to 27% of pediatric MLL-rearranged leukemias. For the first time, we report comprehensive genomewide EVI1 binding and whole transcriptome gene deregulation in leukemic cells using a combination of ChIP-Seq and RNA-Seq expression profiling. We found disruption of terminal myeloid differentiation and cell cycle regulation to be prominent in EVI-induced leukemogenesis. Specifically, we identified EVI1 directly binds to and downregulates the master myeloid differentiation gene <i>Cebpe</i> and several of its downstream gene targets critical for terminal myeloid differentiation. We also found EVI1 binds to and downregulates <i>Serpinb2</i> as well as numerous genes involved in the Jak-Stat signaling pathway. Finally, we identified decreased expression of several ATP-dependent P2X purinoreceptors genes involved in apoptosis mechanisms. These findings provide a foundation for future study of potential therapeutic gene targets for EVI1-induced leukemia.</p></div

Distribution of significant EVI1 binding sites.

<p>The distribution of the 16,745 significant EVI1 ChIP-Seq peaks was plotted against all known transcription start sites (TSS) of annotated genes within the mouse genome using the Stanford Bejerano Lab Great Genomic Regions Enrichment Analyses Tool. EVI1 significantly bound within 5kb of 2,430 annotated genes.</p

Significantly enriched KEGG pathways for genes with aberrant expression levels in EVI1 leukemic cells.

<p>DAVID analysis was performed for significantly upregulated and downregulated genes in both the <i>Evi1</i> overexpressing DA-1 and NFS-60 leukemic cell lines.</p

Significantly deregulated genes based on RNA-Seq demonstrated in both murine cell lines (DA-1 and NFS-60).

<p>Significantly deregulated genes based on RNA-Seq demonstrated in both murine cell lines (DA-1 and NFS-60).</p

Quantitative PCR primer sets used to confirm novel EVI1 target genes.

<p>Quantitative PCR primer sets used to confirm novel EVI1 target genes.</p

Significant EVI1 DNA binding peaks.

<p>Analysis using the UCSC Genome Browser showed the ChIP-Seq EVI1 binding sites demonstrated 10.9% alignment with the whole mouse genome (approximately 5 million reads). 16,745 significant peaks, defined as a difference in number of reads between the control rabbit IgG and EVI1 antiserum antibody yielding a p-value <0.001, were identified based on a Poisson distribution. <b>a)</b> Of the 16,745 generated significant peaks (p<0.001), 45.5% were within introns, 35.0% within distal intergenic region, 7.1% were within the proximal (within 1kb) of the TSS. <b>b)</b> A 500bp DNA sequence extracted around each significant peak was matched to de novo consensus sequence discovery programs. The AGGAAG ETS-like motif was identified and refined in 88% of the significant EVI1 ChIP-Seq binding sites.</p

Significant downregulation of <i>Cebpe</i> in human <i>Evi1</i> overexpressed leukemic cells.

<p><b>a)</b> Lanes 1 and 2 are beta-actin positive controls for U937 wildtype and U937+<i>Evi1</i> cells, respectively. Lane 3 sample is U937 wildtype cells (without <i>Evi1</i> overexpression) and Lane 4 sample is U937 with <i>Evi1</i> overexpression. <i>Cebpe</i> is downregulated in EVI1 overexpressed U937 human leukemic cells (Lane 4). <b>b)</b> Quantitative RT-PCR shows significant downregulation of <i>Cebpe</i> in <i>Evi1</i> overexpressed human leukemic cells. The y-axis value denotes the relative levels of RNA expression based on normalized Ct values. U937+<i>Evi1</i> cells had 8 point increase in Ct value (or 256 fold decrease) compared to the U937 wildtype cells (p<0.001).</p