Деградація земель у Калуському районі внаслідок сольового забруднення

Показано, що джерелами деградації ґрунтів внаслідок, їх засолення, є солевідвали Домбровського кар’єру. Основними чинниками, що призводять до деградації є вітрова і водна ерозія. Досліджено, що основну роль в засоленні ґрунтового покриву відіграють процеси дифузії. Встановлено, що площа засолення (деградація) ґрунтів у декілька разів перевищує площу солевідводів.Показано, что источниками деградации почв вследствие их засоления, являются солеотвалы Домбровского карьера. Основными факторами, которые приводят к деградации является ветровая и водная эрозия. Доказано, что основную роль в засолении почвенного покрова играют процессы диффузии. Установлено, что площадь засоления (деградация) почв в несколько раз превышает площадь солеотвалов.In the article is shown that the sources of land degradation occurs because of their salinity and salt piles from Dombrowsky career. The main factors that lead to the degradation are wind and water erosion. It is investigated that the main role in the salinity of soil processes play diffusion. It is established that the area of salinity (degradation) of soil several times salt piles are

Additional file 1: Figure S1. of Preferential Y-Y pairing and synapsis and abnormal meiotic recombination in a 47,XYY man with non obstructive azoospermia

Unsynapsed regions of the sex chromosomes are stained positive for γH2AX while synapsed regions remained unstained when the YY associated with X chromosome in spermatocytes of 47,XYY male. Pachytene spermatocytes immunostained for γ-H2AX (Green), MLH1 (Green), CREST (Blue) and SYCP3 (Red). (A) γ-H2AX signals were not detected in the region where YY were synapsed YY while γ-H2AX signals were detectable in unsynapsed regions of Y and X chromosomes. (B) Enlarged area from (A). (C) A schematic configuration of the sex chromosomes from the cell shown in B. white arrow indicating the region with synapsed YY. (TIF 4775 kb

miR-214-mediated downregulation of RNF8 induces chromosomal instability in ovarian cancer cells

<div><p>Defective DNA damage response (DDR) is frequently associated with carcinogenesis. Abrogation of DDR leads to chromosomal instability, a most common characteristic of tumors. However, the molecular mechanisms underlying regulation of DDR are still elusive. The ubiquitin ligase RNF8 mediates the ubiquitination of γH2AX and recruits 53BP1 and BRCA1 to DNA damage sites which promotes DDR and inhibits chromosomal instability. Though RNF8 is a key player involved in DDR, regulation of its expression is still poorly understood. Here, we show that miR-214 could abrogate DDR by repressing RNF8 expression through direct binding to 3′-untranslated region (3′ UTR) of RNF8 mRNA in human ovarian cancer cells. Antagonizing miR-214 by expressing its inhibitors in A2780 cells significantly increased RNF8 expression and thus promoted DNA damage repair. Consistent with the role of miR-214 in regulating RNF8 expression, the impaired DNA repair induced by miR-214 overexpression can be rescued by overexpressing RNF8 mRNA lacking the 3′ UTR. Together, our results indicate that down-regulation of RNF8 mediated by miR-214 impedes DNA damage response to induce chromosomal instability in ovarian cancers, which may facilitate the understanding of mechanisms underlying chromosomal instability.</p></div

Additional file 4: Table S1. of Anaconda: AN automated pipeline for somatic COpy Number variation Detection and Annotation from tumor exome sequencing data

Evaluation of performance gain of Anaconda. (DOCX 16 kb

Pearson’s correlation and multiple stepwise linear regression analysis of CCL20 associated with classic GD diagnostic parameters.

<p><i>r</i>, correlation coefficient; β, Regression coefficient; SE, standard error.</p

β3 integrin receptor medicated OPN induction of CCL20 expressions.

<p>(A) CCL20 gene expressions in isolated CD4+T cells from 8 hCD and 8 uGD in the presence or absence of 1 µg/ml rOPN for 12 h. (B) Expressions of OPN receptors on CD4+ T cells from uGD patients, eGD patients, nGD patients and hCD. (C) β3 integrin receptor antibody blocked induction of CCL20 mRNA and protein levels by OPN. The freshly isolated PBMCs were cultured in the presence or absence of 1 µg/ml rOPN for 12 h. Blocking Abs to integrin β3, or its isotype control mAb (IgG) were added at 5 µg/ml. Supernatants from cultures and CD4+T cells separated from PBMCs were used to analyze the expression levels of CCL20. Shown are representative results from 3 independent experiments with separate specimens. All data were presented as mean±SEM. *, <i>P</i><0.05; ***, <i>P</i><0.001, versus medium control; #, <i>P</i><0.05 versus Ctrl mAb.</p

A new working model of responsive anti-inflammatory cytokine and housekeeping cytokine.

<p>Homeostatic tissues express “house-keeping” anti-inflammatory cytokines TGF-β1, TGF-β2, TGF-β3 to prevent it from initiation of inflammation. When tissues get inflamed, proinflammatory factors may stimulate tissues to express “responsive” anti-inflammatory cytokines such as IL-35 by specific transcription factors to counteract inflammation response. Furthermore, ARE binding proteins and MicroRNAs are responsible of the quick degradation of IL-35 mRNA afterwards, by which IL-35 achieve non-constitutive expression status in tissues again.</p

Plasma CCL20 and CCL20 mRNA in CD4+T cells from GD patients and normal controls.

<p>(A) Distribution of plasma CCL20 levels in 35 healthy control donors, 50 untreated GD patients, 15 euthyroid GD patients and 12 TRAb-negative GD patients. Median values, interquartile ranges and ranges are denoted by horizontal bars, boxes and vertical bars, respectively. (B) CCL20 mRNA levels in CD4+T cells from healthy controls, uGD, eGD and nGD patients. Data were presented as mean±SEM.*, <i>P</i><0.05; **, <i>P</i><0.01.</p

Effect of OPN on induction of CCL20 expression was mediated by IL-17, NF-κB and MAPK pathways.

<p>(A, B) PBMCs were isolated from normal subjects and treated with rOPN (1 µg/ml) at different time point. The IL-17 mRNA expression in purified CD4+T cells (A) and protein level in culture medium (B) were analyzed. Induction of CCL20 mRNA in CD4+T cells (C) and protein levels in culture medium (D) by OPN was blocked by antibody against IL-17, and inhibitors of IKK and MAPKs. Shown are representative results from 3 independent experiments with separate specimens. All data were presented as mean±SEM. *, <i>P</i><0.05 versus OPN; # <i>P</i><0.05 versus medium control; ns>0.05 versus OPN.</p

IL-35 subunit mRNAs have been shown to be induced by stimulation in various human cell types.

<p>IL-35 subunit mRNAs have been shown to be induced by stimulation in various human cell types.</p