The Proteins from Sika deer antler as potential modulators on cisplatin-induced cytotoxicity in human embryonic kidney 293 cells

<p>Our study aimed to investigate the protective role of SDAPR on cisplatin-induced cytotoxicity and its’ possible mechanism in HEK293 cells. Cell viability was measured by MTT assay. Oxidative stress (SOD, GSH, LDH and MDA), inflammatory factors (TNF-α and IL-6) and apoptosis-related proteins (caspase-3, Bax, Bcl-2) expression were measured. The apoptotic cells were observed by TUNEL staining. Our study results indicated that non-cytotoxic levels of SDAPR significantly increased viability rate (LD₅₀ value of cisplatin is 20 μM), which improved antioxidant defence, attenuated apoptosis by decreasing expression levels of cleaved-caspase-3 and Bax, increasing Bcl-2 expression and inhibiting apoptotic positive cells in HEK 293 cells. In addition, SDAPR treatment markedly inhibited the levels of TNF-α and IL-6. In conclusion, Sika deer antler protein, a potential modulator, could alleviate cisplatin-induced cytotoxicity in HEK 293 cells.</p

Allelochemicals from the Rhizosphere Soil of Cultivated Astragalus hoantchy

Astragalus hoantchy, a widely cultivated medicinal plant species in traditional Chinese and Mongolian medicine, has been often hampered by replant failure during cultivation, like many other herbs of the genus Astragalus. Root aqueous extracts of Astragalus herbs were reported to exhibit allelopathic activity against other plants and autotoxic activity on their own seedlings, but the allelochemicals released by Astragalus plants have not been specified so far. Ten compounds were isolated from the rhizosphere soil extract of cultivated A. hoantchy and elucidated by spectroscopic analysis. Compounds <b>1</b>–<b>6</b> observably showed allelopathic activity against Lactuca sativa seedlings and autotoxic activity against A. hoantchy seedlings. The isolated compounds were further confirmed and quantified by high-performance liquid chromatography (HPLC) in the rhizosphere soil, with a total concentration of 9.78 μg/g (dry weight). These results specify and verify the allelochemicals released by cultivated A. hoantchy into the soil environment, which may provide new insights into the allelopathic mechanisms of this medicinal plant and probably assist in clarifying the replant problems of Astragalus plants

Cycloartane-type triterpenoids from <i>Astragalus hoantchy</i> French.

<p>A new cycloartane-type triterpenoid, 3β-acetoxy-9β,19-cyclolanost-24<i>E</i>-ene-1α,16β-diol-27-<i>O</i>-β-d-glucopyranoside (<b>1</b>), together with three known ones (<b>2</b>–<b>4</b>) were isolated from the aerial parts of <i>Astragalus hoantchy</i>. The structure of compound <b>1</b> was elucidated by detailed spectroscopic analyses including 1D and 2D NMR, HRESIMS, and IR experiments. All of the four compounds were isolated from <i>A. hoantchy</i> for the first time. The antifungal activities of compounds <b>1</b>–<b>4</b> against a phytopathogenic fungus (<i>Alternaria solani</i>) were evaluated with the result that compound <b>3</b> exhibited a moderate activity with an IC₅₀ value of 284 μg/mL.</p

Synthesis of co-crystals of meloxicam with carboxylic acids by grinding

Search strategy. (DOCX 13 kb

Additional file 1 of Model containing sarcopenia and visceral adiposity can better predict the prognosis of hepatocellular carcinoma: a multicenter study

Additional file 1