83 research outputs found
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-7
, induced; 3, recombinant bacterium (containing gene), non-induced; 4, recombinant bacterium (containing gene), induced; 5, purified GST-GBSV1-NSN fusion protein; 6, purified GBSV1-NSN.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p
Amino acid alignments of shrimp Ago1 isoforms and Ago homologs from other species.
<p>The conserved PAZ and PIWI domains were boxed. Amino acid differences between shrimp Ago1 isoforms were highlighted with asterisks. <i>Homo sapiens</i>, Hs Ago1 (GenBank accession no. NP_036331.1); <i>Mus musculus</i>, Mm Ago1 (AAI29916.1); <i>Tribolium castaneum</i>, Tc Ago1 (XP_971295.2); <i>Bombyx mori</i>, Bm Ago1 (NP_001095931.1); <i>Drosophila melanogaster</i>, Dm Ago1 (NP_725341.1); Dm Ago2 (NP_Q9VUQ5); <i>Apis mellifera</i> Am Ago1 (XP_624444.3); <i>Litopenaeus vannamei</i>, Lv Ago1 (NP_ADK25180.1); Lv Ago2 (NP_ADK25181.1); <i>Marsupenaeus japonicus</i>, Mj Ago1.</p
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-0
Da Ba01 [GenBank: ], phage SPP1 [GenBank: ], phage 370.3 [GenBank: ], phage EJ-1 [GenBank: ] and phage phiC2 [GenBank: ]. The DnaD-like domain of GBSV1-NSN held positions 158–227.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p
Contribution of the Argonaute-1 Isoforms to Invertebrate Antiviral Defense
<div><p>Argonaute (Ago) protein, the central component of the RNA interference (RNAi) pathway, plays important roles in host innate antiviral immunity. Most organisms harbor a large number of different Ago proteins and isoforms; however, the roles of Ago isoforms in immune defense against pathogens remain unclear. In the present study, three Argonaute-1 (Ago1) isoforms, termed Ago1A, Ago1B, and Ago1C, were found in <em>Marsupenaeus japonicus</em> shrimp. Quantitative real-time PCR (polymerase chain reaction) revealed that isoforms Ago1A and Ago1B containing an insertion sequence in the PIWI domain, were significantly up-regulated in lymphoid organ and hemolymph, and also upon white spot syndrome virus (WSSV) challenge, indicating the involvement of Ago1A and Ago1B in antiviral immunity. The results showed that silencing of Ago1A with a sequence-specific siRNA led to a significant increase of WSSV loads. It was revealed that knockdown of Ago1B mRNA by 37–70% resulted in higher virus loads in shrimp. However, upon silencing Ago1B by more than 85%, a two-fold increase in Ago1A mRNA was observed but viral load was the same as untreated controls challenged with WSSV, suggesting that the simultaneous up-regulation of Ago1A might compensate for the loss of Ago1B. These data indicated that Ago1A played more important roles in the antiviral immune response than Ago1B. The simultaneous inhibition of Ago1A and Ago1B resulted in a greater increase in viral loads than Ago1A or Ago1B alone, indicating that Ago1A and Ago1B isoforms were involved in shrimp antiviral immunity. It was revealed that Ago1C had no effect on virus infection. Therefore, the current study presented the first report on the contribution of Ago isoforms in the invertebrate defense against virus infection.</p> </div
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-8
Se I (B, C and D) was also included as positive control. The protein solutions were shown on the top and the nucleic acids were indicated on the right. 1 μg of nucleic acids were respectively incubated with 1.5 μg of the purified GBSV1-NSN protein in 20 μl of reaction buffer at 37°C for six hours.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p
Identification of shrimp Ago1 isoforms.
<p>Schematic diagram of three isoforms (Ago1A, Ago1B, and Ago1C) of shrimp Ago1 gene. The numbers show the sites of Ago1-fragment 1 and Ago1-fragment 2 in Ago1.</p
Specificities of siRNAs targeting Ago1 isoforms.
<p>S2 cells were transiently co-transfected with the Flag-tagged Ago1 isoform constructs and the isoform-specific siRNAs. At 48 h after transfection, cell lysates were analyzed using western blot with anti-FLAG antibody. The β-actin was used as a control. Lane headings showed the FLAG-tagged Ago1 isoforms and the isoform-specific siRNAs. The Ago1A/B-siRNA could specifically target both Ago1A and Ago1B. The antibodies used were indicated on the left.</p
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-5
S. The nucleic acid concentrations were varied in the range of 0–100 μg/ml. All nuclease activity assays were conducted at 60°C and pH 7.5. Each point represented the mean of triplicate assays and the error bars represented the standard deviations.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-6
Da Ba01 [GenBank: ], phage SPP1 [GenBank: ], phage 370.3 [GenBank: ], phage EJ-1 [GenBank: ] and phage phiC2 [GenBank: ]. The DnaD-like domain of GBSV1-NSN held positions 158–227.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p
Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-1
, induced; 3, recombinant bacterium (containing gene), non-induced; 4, recombinant bacterium (containing gene), induced; 5, purified GST-GBSV1-NSN fusion protein; 6, purified GBSV1-NSN.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p
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