2,320 research outputs found
Plane-Based Optimization of Geometry and Texture for RGB-D Reconstruction of Indoor Scenes
We present a novel approach to reconstruct RGB-D indoor scene with plane
primitives. Our approach takes as input a RGB-D sequence and a dense coarse
mesh reconstructed by some 3D reconstruction method on the sequence, and
generate a lightweight, low-polygonal mesh with clear face textures and sharp
features without losing geometry details from the original scene. To achieve
this, we firstly partition the input mesh with plane primitives, simplify it
into a lightweight mesh next, then optimize plane parameters, camera poses and
texture colors to maximize the photometric consistency across frames, and
finally optimize mesh geometry to maximize consistency between geometry and
planes. Compared to existing planar reconstruction methods which only cover
large planar regions in the scene, our method builds the entire scene by
adaptive planes without losing geometry details and preserves sharp features in
the final mesh. We demonstrate the effectiveness of our approach by applying it
onto several RGB-D scans and comparing it to other state-of-the-art
reconstruction methods.Comment: in International Conference on 3D Vision 2018; Models and Code: see
https://github.com/chaowang15/plane-opt-rgbd. arXiv admin note: text overlap
with arXiv:1905.0885
Genetic Transformation of Papaya (Carica Papaya, L.) Cultivar Kapoho by Particle Bombardment
Papaya transformation systems were developed by Fitch et al. (1991) at the University of Hawaii, and transgenic 'Sunset' papayas with papaya ringspot virus (PRV) coat protein (cp) gene showed complete resistance to papaya ringspot virus (PRV) in the field tests (Manshardt et al, 1994). In our studies, we transformed 'Kapoho' papaya, the major crop on the Big Island, Hawaii, based on Fitch’s (1991) papaya transformation systems, and obtained transgenic 'Kapoho' papaya plants.
The coat protein (cp) gene of PRV, along with a kanamycin selective marker gene (neomycin phosphotransferase, NPTII) and a Pglucuronidase (GUS) reporter gene, were constructed into the same plasmid vector by our collaborators at Cornell University and transformed into papaya tissue by particle bombardment. Transgenic 'Kapoho' papaya plants were obtained following somatic embryogenesis from hypocotyl callus on kanamycin selective medium and showed GUS positive expression.
Immature zygotic embryos were excised and bombarded with gold particles. Following different treatments of indole-3-butyric acid (IBA), chimeric hypocotyls were harvested on germination medium 20 days after bombardment. Somatic embryogenesis from sections of chimeric transgenic hypocotyls occurred on induction medium and the transgenic embryos were cultured on selective induction medium or maturation medium with different concentrations of kanamycin for eight months. Then, the embryos were regenerated on germination medium without kanamycin.
GUS was assayed in all experimental steps, and different GUS positive results were observed at different developmental stages. ELISA assays of coat protein and NPTII in chimeric transgenic hypocotyls showed positive expression and a high efficiency of transformation
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