4,279 research outputs found
A spectral line survey of IRC +10216 between 13.3 and 18.5 GHz
A spectral line survey of IRC +10216 between 13.3 and 18.5 GHz is carried out
using the Shanghai Tian Ma 65 m Radio Telescope (TMRT-65m) with a sensitivity
of < 7 mK. Thirty-five spectral lines of 12 different molecules and radicals
are detected in total. Except for SiS, the detected molecules are all
carbon-chain molecules, including HC3N, HC5N, HC7N, HC9N, C6H, C6H-, C8H, SiC2,
SiC4, c-C3H2 and l-C5H. The presence of rich carbon-bearing molecules is
consistent with the identity of IRC +10216 as a carbon-rich AGB star. The
excitation temperatures and column densities of the observed species are
derived by assuming a local thermodynamic equilibrium and homogeneous
conditions.Comment: This is the authors' version of the manuscript; 16 pages, 5 figures,
6 tables; Accepted for publication in A&A 8/17/201
A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division
The R2R3-MYB transcription factor FOUR LIPS (FLP) controls the stomatal terminal division through transcriptional repression of the cell cycle genes CYCLIN-DEPENDENT KINASE (CDK) B1s (CDKB1s), CDKA; 1, and CYCLIN A2s (CYCA2s). We mutagenized the weak mutant allele flp-1 seeds with ethylmethane sulfonate and screened out a flp-1 suppressor 1 (fsp1) that suppressed the flp-1 stomatal cluster phenotype. FSP1 encodes RPA2a subunit of Replication Protein A (RPA) complexes that play important roles in DNA replication, recombination, and repair. Here, we show that FSP1/RPA2a functions together with CDKB1s and CYCA2s in restricting stomatal precursor proliferation, ensuring the stomatal terminal division and maintaining a normal guard-cell size and DNA content. Furthermore, we provide direct evidence for the existence of an evolutionarily conserved, but plant-specific, CDK-mediated RPA regulatory pathway. Serine-11 and Serine-21 at the N terminus of RPA2a are CDK phosphorylation target residues. The expression of the phosphorylation-mimic variant RPA2a(S11,21/D) partially complemented the defective cell division and DNA damage hypersensitivity in cdkb1;1 1;2 mutants. Thus, our study provides a mechanistic understanding of the CDK-mediated phosphorylation of RPA in the precise control of cell cycle and DNA repair in plants
Discriminating different scenarios to account for the cosmic excess by synchrotron and inverse Compton radiation
The excesses of the cosmic positron fraction recently measured by PAMELA and
the electron spectra by ATIC, PPB-BETS, Fermi and H.E.S.S. indicate the
existence of primary electron and positron sources. The possible explanations
include dark matter annihilation, decay, and astrophysical origin, like
pulsars. In this work we show that these three scenarios can all explain the
experimental results of the cosmic excess. However, it may be difficult
to discriminate these different scenarios by the local measurements of
electrons and positrons. We propose possible discriminations among these
scenarios through the synchrotron and inverse Compton radiation of the primary
electrons/positrons from the region close to the Galactic center. Taking
typical configurations, we find the three scenarios predict quite different
spectra and skymaps of the synchrotron and inverse Compton radiation, though
there are relatively large uncertainties. The most prominent differences come
from the energy band MHz for synchrotron emission and GeV for inverse Compton emission. It might be able to discriminate at least
the annihilating dark matter scenario from the other two given the high
precision synchrotron and diffuse -ray skymaps in the future.Comment: published in Pr
Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells
<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [<sup>3</sup>H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.</p> <p>Results</p> <p>Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.</p> <p>Conclusions</p> <p>IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines <it>in vitro</it>, and it provides an experimental basis for c-Met-targeted therapy towards <it>in vivo </it>testing.</p
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Changes of functional connectivity in the left frontoparietal network following aphasic stroke
Language is an essential higher cognitive function supported by large-scale brain networks. In this study, we investigated functional connectivity changes in the left frontoparietal network (LFPN), a language-cognition related brain network in aphasic patients. We enrolled 13 aphasic patients who had undergone a stroke in the left hemisphere and age-, gender-, educational level-matched controls and analyzed the data by integrating independent component analysis (ICA) with a network connectivity analysis method. Resting state functional magnetic resonance imaging (fMRI) and clinical evaluation of language function were assessed at two stages: 1 and 2 months after stroke onset. We found reduced functional connectivity between the LFPN and the right middle frontal cortex, medial frontal cortex, and right inferior frontal cortex in aphasic patients as compared to controls. Correlation analysis showed that stronger functional connectivity between the LFPN and the right middle frontal cortex and medial frontal cortex coincided with more preserved language comprehension ability after stroke. Network connectivity analysis showed reduced LFPN connectivity as indicated by the mean network connectivity index of key regions in the LFPN of aphasic patients. The decreased LFPN connectivity in stroke patients was significantly associated with the impairment of language function in their comprehension ability. We also found significant association between recovery of comprehension ability and the mean changes in intrinsic LFPN connectivity. Our findings suggest that brain lesions may influence language comprehension by altering functional connectivity between regions and that the patterns of abnormal functional connectivity may contribute to the recovery of language deficits
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