NLO QCD corrections to Single Top and W associated production at the LHC with forward detector acceptances

In this paper we study the Single Top and W boson associated photoproduction via the main reaction $\rm pp\rightarrow p\gamma p\rightarrow pW^{\pm}t+Y$ at the 14 TeV Large Hadron Collider (LHC) up to next-to-leading order (NLO) QCD level assuming a typical LHC multipurpose forward detector. We use the Five-Flavor-Number Schemes (5FNS) with massless bottom quark assumption in the whole calculation. Our results show that the QCD NLO corrections can reduce the scale uncertainty. The typical K-factors are in the range of 1.15 to 1.2 which lead to the QCD NLO corrections of 15$$ to 20$$ correspond to the leading-order (LO) predictions with our chosen parameters.Comment: 41pages, 12figures. arXiv admin note: text overlap with arXiv:1106.2890 by other author

A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera

<p>Abstract</p> <p>Background</p> <p>The orthologs of eukaryotic initiation factor 5C (eIF5C) are essential to the initiation of protein translation, and their regulation during development is not well known.</p> <p>Results</p> <p>A cDNA encoding a polypeptide of 419 amino acids containing an N-terminal leucine zipper motif and a C-terminal eIF5C domain was cloned from metamorphic larvae of <it>Helicoverpa armigera</it>. It was subsequently named <it>Ha-eIF5C</it>. Quantitative real-time PCR (QRT-PCR) revealed a high expression of the mRNA of <it>Ha-eIF5C </it>in the head-thorax, integument, midgut, and fat body during metamorphosis. Immunohistochemistry suggested that Ha-eIF5C was distributed into both the cytoplasm and the nucleus in the midgut, fat body and integument. <it>Ha-eIF5C </it>expression was upregulated by 20-hydroxyecdysone (20E). Furthermore, the transcription of <it>Ha-eIF5C </it>was down regulated after silencing of <it>ecdysteroid receptor (EcR) </it>or <it>Ultraspiracle protein (USP) </it>by RNAi.</p> <p>Conclusion</p> <p>These results suggested that during metamorphosis of the cotton bollworm, <it>Ha-eIF5C </it>was upregulated by 20E through the EcR and USP transcription factors.</p

To investigate the reasons and nursing strategies of postoperative return to ICU in patients with severe valvular disease

目的  探讨重症瓣膜置换病人术后再次非计划性返回ICU的重返率及原因。根据原因分析建立最佳护理模式及干预策略，通过护理干预手段降低重返率，提高治愈率及患者术后康复质量。方法  回顾性分析2010年6月—2015年6月间150例重症瓣膜置患者术后重返ICU患者的临床资料、分析重返的原因。结果  150例重症瓣膜置换患者术后非计划重返ICU的患者有17例，重返率为11.3%。重返的主要原因是呼吸困难、恶性心律失常、低心排、循环负荷过重、家属照护压力大主动要求重返ICU等。结论  制定合理的转出标准；合理配置人力资源，动态监测，密切观察患者生命体征、出入量平衡、电解质平衡；提供个性化健康指导提高患者及家属的遵医行为是降低ICU重返率的有效措施。Objective: To investigate the return rate and causes of postoperative non - planned return to ICU in patients with severe valve replacement. According to the analysis of the reasons, establish the best nursing model and intervention strategies, through the nursing intervention to reduce the return rate, and improve the cure rate and postoperative rehabilitation quality. Methods: Retrospective analysis of clinical data of 150 cases of severe valvular replacement patients who returned to ICU in June, June 2010, and the reasons for the return of ~2015. Results: 150 cases of patients with severe heart valve replacement patients, among them 150 cases had no plan to return to ICU, the return rate was 11.3%. The main reason to return to ICU is the difficulty of breathing, malignant arrhythmia, low cardiac output, overweight, family care pressure and so on n. Conclusion: To establish a reasonable standard of transfer; the rational allocation of human resources, dynamic monitoring, close observation of vital signs of patients, access to balance, electrolyte balance; providing personalized health guidance to improve the compliance behavior of patients and their families are the effective measures to reduce the ICU readmission rate.

Phenol Adsorption on Nitrogen-Enriched Activated Carbon from Wood Fiberboard Waste

Nitrogen-enriched activated carbons were prepared from wood fiberboard waste using 50% potassium hydroxide solution. Activated carbons were obtained with an impregnation ratio (gram chemical agent/gram wood fiberboard waste) of 3 in 850°C activation temperature carbonized for 60 min. Nitrogen content in activated carbon was 1.33% by analysis. Effects of contact time, pH, adsorbent dosage level, and temperature on phenol adsorption capacity of activated carbons were investigated. Adsorption equilibrium was achieved within 100 min at the given phenol concentration of 250 mg/L. When 0.1 g of the carbon absorbent and 100 mL of phenol solution at 250 mg/L were used, maximum adsorption capacity of phenol on activated carbon can reach 207 mg/g. The kinetics of phenol adsorption followed nicely the pseudo-second-order rate expression. In the adsorption isotherm, the Langmuir model fit better than the Freundlich model in phenol adsorption. This study suggests that nitrogen-enriched activated carbon prepared from wood fiberboard waste can be used effectively for removal of phenol compounds from aqueous solutions

Ardipusilloside I induces apoptosis by regulating Bcl-2 family proteins in human mucoepidermoid carcinoma Mc3 cells

BACKGROUND: Ardisia pusilla A. DC., family Myrsinaceae, is a traditional Chinese medicine named Jiu Jie Long with a variety of pharmacological functions including anti-cancer activities. In this study, we purified a natural triterpenoid saponin, ardipusilloside I, from Ardisia pusilla, and show that it exhibits inhibitory activities in human mucoepidermoid carcinoma Mc3 cells. We also investigated the underlying mechanisms of proliferation inhibition that ardipusilloside I exerts on Mc3 cells. METHODS: MTT test was used to detect cell proliferation. Cell apoptosis was detected by transmission electron microscopy, Hoechst-33342 staining, DNA fragmentation detection, and flow cytometry. We also used western blot analysis to detect the potential mechanisms of apoptosis. RESULTS: Ardipusilloside I affected the viability of Mc3 cells in a dose- and time-dependent manner. The IC50 of ardipusilloside I was approximately 9.98 μg/ml at 48 h of treatment. Characteristic morphological changes of apoptosis, including nuclear condensation, boundary aggregation and splitting, and DNA fragmentation, were seen after treatment with 10 μg/ml ardipusilloside I for 48 h. Western blots demonstrated that ardipusilloside I caused Mc3 cell death through the induction of apoptosis by downregulation of Bcl-2 protein levels and upregulation of Bax and caspase-3 protein levels. CONCLUSIONS: Our results revealed that ardipusilloside I could be a new active substance for mucoepidermoid carcinoma treatment. We demonstrated that the potential mechanism of inhibition might be through the induction of apoptosis by regulation of Bcl-2 family protein levels. This suggests a further rationale for the development of ardipusilloside I as an anti-cancer agent

Immune responses of Helicoverpa armigera to different kinds of pathogens

<p>Abstract</p> <p>Background</p> <p>Insects react against pathogens through innate immunity. The cotton bollworm <it>Helicoverpa armigera </it>(<it>H. armigera</it>) is an important defoliator and an extremely destructive pest insect of many crops. The elucidation of the mechanism of the immune response of <it>H. armigera </it>to various pathogens can provide a theoretical basis for new approaches to biologically control this pest.</p> <p>Results</p> <p>Four kinds of pathogens <it>Bacillus thuringiensis</it>, <it>Klebsiella pneumoniae</it>, <it>Candida albicans</it>, and <it>Autographa californica </it>multiple nucleocapsid nucleopolyhedrovirus harbored green fluorescence protein and polyhedron (<it>Ac</it>MNPV-GFP) were used to challenge the insect. The cellular and humoral immune responses to the pathogens were analyzed in the challenged <it>H. armigera</it>. The results show that in the five kinds of haemocytes, only granulocytes phagocytized the Gram-negative and Gram-positive bacteria and fungi. All haemocytes can be infected by <it>Ac</it>MNPV. Fourteen immune-related genes including pattern recognition receptors (PRRs) such as peptidoglycan recognition proteins (<it>HaPGRP </it>and <it>HaPGRP C</it>) and Gram-Negative Bacteria-Binding Protein (<it>HaGNBP</it>), and antimicrobial peptides (AMPs) such as <it>cecropin-1, 2 </it>and <it>3 </it>(<it>HaCec-1, 2 </it>and <it>3</it>), <it>lysozyme </it>(<it>HaLys</it>), attacin (<it>HaAtt</it>), <it>gallerimycin-like </it>(<it>HaGall</it>), <it>gloverin-like </it>(<it>HaGlo</it>), <it>moricin-like </it>(<it>HaMor</it>), cobatoxin-like (<it>HaCob</it>), <it>galiomicin-like </it>(<it>HaGali</it>), and <it>immune inducible protein </it>(<it>HaIip</it>) appeared in different expression profiles to different pathogen infections. The transcripts of 13 immune related genes (except <it>HaPGRPC</it>) are obviously up-regulated by Gram-positive bacteria. <it>HaCec-1 and 3, HaMor, HaAtt, HaLys</it>, <it>HaIip</it>, <it>HaPGRP </it>and <it>HaGNBP </it>are greatly up-regulated after fungal infection. <it>HaGNBP, HaCec-2, HaGall, HaGlo, HaMor, HaCob, HaGali </it>obviously increased in Gram-negative bacterial infection. Only five genes, <it>HaGNBP, HaCec-1</it>, <it>HaGali</it>, <it>HaGlo</it>, and <it>HaLys</it>, are weakly up-regulated after viral infection. The AMP transcripts had higher expression levels than the PRR transcripts after the microbial challenge.</p> <p>Conclusions</p> <p>These data suggest that the granulocytes are the major phagocytes in <it>H. armigera</it>. All haemocytes can be infected by <it>Ac</it>MNPV. The transcripts of 14 immune related genes have different expression patterns in <it>H. armigera </it>infected by different pathogens, which means that the immune-related genes may have different functions against various kinds of pathogens.</p

Medical personnel to the implementation of patient care integration management pattern recognition

目的  调查医护人员对实施医护一体化共同管理病人模式的认可程度，寻找实施过程中存在的问题，以更好的提高病人的管理质量，得到患者的认可。方法  对本院各抽取2个科所有医、护、技术人员共63名医护人员进行问卷调查。结果  100%的医护人员知晓科室目前开展了医护一体化工作模式，医护一体化小组的成员组成，熟悉医护一体化工作模式的流程，人员的岗位职责及认为实施医护一体化工作模式后医、护、技工作配合更加默契。98.7%医护人员认为实施医护一体化工作模式后病人的医疗护理质量得到明显提高。98%的医护人员认为实施医护一体化管理病人模式后自我效能感得到明显提高。97.8%自我管理病人的能力得到提高且认同实施医护一体化工作模式。结论  医护一体化管理病人模式的实施提高了医护人员的工作积极性，拓展了知识，提高了临床思维能力，得到了广大医护人员的认可。同时还应加大宣传、培训的力度，使更多的医护人员了解医护一体化的益处。Objective: To Investigate the implementation of management mode among the medical personnel, to find the existing problems in the implementation process, to manage better quality accepted by patients. Methods: Set a total of 63 questionnaires for all the medical technical personnel. Results: 100% staffs know that now integration mode is carried out in the department, member of medical integration group, familiar with the medical work pattern of the integration process, the post responsibility, and the implementation of medical integration of medical integration made. Doctors and nurses technicians work with more understanding. 98.7% staff believes that the quality of medical care of the patient was obviously improved after the implementation of medical integration work mode. 98% the medical staff believes that self-efficacy has been improved obviously. 97.8% the medical staff think that self-management ability of patients is improved and the implementation of the medical integration mode identification. Conclusion: The implementation model of integrated management of patient care improves the medical staffs’ activeness, expanding knowledge, improving the clinical thinking ability, the majority of doctors and nurses recognition. At the same time publicity and training should be enhanced so that more medical personnel understand the medical integration efforts

Identification and expression profile of a putative basement membrane protein gene in the midgut of Helicoverpa armigera

<p>Abstract</p> <p>Background</p> <p>The midgut undergoes histolysis and remodeling during the larval to adult transition in holometabolous insects, but the molecular mechanisms underlying this process are not well understood.</p> <p>Results</p> <p>Using Suppression Subtractive Hybridization (SSH), we identified a 531 bp cDNA predicted to encode a 176 amino acid protein, which we call <it>hmg176</it>. Northern and western blot analysis suggested that high levels of <it>hmg176 </it>are expressed in the midgut during molting, but not during metamorphosis. HMG176 protein was detected by immunofluorescence within the membrane of fat bodies and the basement membrane of the midgut of both molting and feeding larvae, but not in metamorphically committed larvae. <it>In situ </it>hybridization revealed that <it>hmg176 </it>transcripts mainly localized to the columnar cells of the midgut. Interestingly, a non-steroidal ecdysone agonist, RH-2485, significantly upregulated expression of <it>hmg176</it>.</p> <p>Conclusion</p> <p>These observations suggest that <it>hmg176 </it>encodes a larval-specific protein that may participate in sustaining larval midgut during larval development, possibly in response to ecdysteroid <it>in vivo</it>. This study will enlighten our understanding of the molecular mechanisms of tissue histolysis during metamorphosis.</p

Analyzing the formation of normal and abnormal O waves in thoracic impedance graph using the impedance change components for aorta, blood vessels in lung and ventricles

Background: Many measurements of thoracic impedance graph show that the small C wave and big O wave appear often for patients with cardiac insufficiency, and the O/C ratio is bigger. And for the normal body, especially a younger one, the bigger O wave may also appear. But since the amplitude of the C wave of a normal body is bigger, the O/C ratio is smaller. The aim of the present paper is to investigate the formation mechanism of the normal and abnormal O waves in thoracic impedance graph. Methods and Results: The thoracic mixed impedance changes are measured with 6 leads consisting of 15 electrodes. The impedance change components for the aorta (AO), blood vessel in left lung (PL), blood vessel in right lung (PR), left ventricle (LV) and right ventricle (RV) are separated from thoracic mixed impedance changes by means of establishing and solving the thoracic impedance equations. The amplitudes of the O and C waves of various impedance change components are measured for 50 normal healthy adults and 34 patients with cardiac insufficiency. The formation mechanism of normal and abnormal O waves in thoracic impedance graph is analyzed using the superposition of the O waves of the above impedance change components. Detection subjects are 50 healthy adults and 34 hospital patients with cardiac insufficiency. (1) Thoracic impedance graph: The O/C ratios of the normal group are significantly smaller than that of the abnormal group, p &lt; 0.001. The O wave of first lead (E1-E1’) is significantly bigger than that of leads 4 and 5 (E4-E4’ and E5-E5’) in the normal group, p &lt; 0.001. (2) The impedance change component: The O waves of the AO, PL, and PR are significantly smaller than that of the LV and RV in the normal group, p &lt; 0.001. The O wave and O/C of the AO, PL and PR of normal group are significantly smaller than that of the abnormal group, p &lt; 0.001. Conclusions: The O wave of the thoracic impedance graph is formed due to the superposition of the O waves of the impedance change components for the aorta, blood vessels in lung and ventricles