A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division

The R2R3-MYB transcription factor FOUR LIPS (FLP) controls the stomatal terminal division through transcriptional repression of the cell cycle genes CYCLIN-DEPENDENT KINASE (CDK) B1s (CDKB1s), CDKA; 1, and CYCLIN A2s (CYCA2s). We mutagenized the weak mutant allele flp-1 seeds with ethylmethane sulfonate and screened out a flp-1 suppressor 1 (fsp1) that suppressed the flp-1 stomatal cluster phenotype. FSP1 encodes RPA2a subunit of Replication Protein A (RPA) complexes that play important roles in DNA replication, recombination, and repair. Here, we show that FSP1/RPA2a functions together with CDKB1s and CYCA2s in restricting stomatal precursor proliferation, ensuring the stomatal terminal division and maintaining a normal guard-cell size and DNA content. Furthermore, we provide direct evidence for the existence of an evolutionarily conserved, but plant-specific, CDK-mediated RPA regulatory pathway. Serine-11 and Serine-21 at the N terminus of RPA2a are CDK phosphorylation target residues. The expression of the phosphorylation-mimic variant RPA2a(S11,21/D) partially complemented the defective cell division and DNA damage hypersensitivity in cdkb1;1 1;2 mutants. Thus, our study provides a mechanistic understanding of the CDK-mediated phosphorylation of RPA in the precise control of cell cycle and DNA repair in plants

The exploration and practice in improving nursing skills training effect under clinical settings

目的  针对护理操作技能培训中临床情境不足、易机械性、习惯性模仿的问题，探索提高培训师临床设置下操作技能培训效果的有效教学方法，使操作技能培训更贴近临床。方法  改变管理角色，变教学督导、检查为“对口辅导”的模式对培训过程、教学方法及考核过程进行现场辅导，并举办培训师经验分享、建立培训交流空间及改革质量监控方法等措施；对辅导型管理前后的考核结果进行自身前后对照。结果  对口辅导前后考核平均成绩具有统计学意义（P＜0.05），辅导型管理前后科室考核与护理部抽考成绩差异性＞±10%的发生例数、临床结合问题不足的例数差异均具有显著性（P＜0.001）；辅导型管理前后不及格例数差异无显著性（P＞0.05）。结论  辅导型管理模式能有效提高培训师临床设置下护理技能培训效果。Objective: In view of the problem of shortage of clinical situation and easy mechanically imitation in nursing skills training , to explore an effective teaching method to improve the trainers’training effect of nursing skills under clinical settings，To make nursing skills more close to clinical. Methods: Change management role from Supervision and inspection to “counterpart counseling”, by performing the teaching process guidance and the examine guidance , establishing the space to share information , reforming the quality control method. to compare the results by setting a self-control between before and after the management of counterpart counseling. Results: Average marks before and after the management of counterpart counseling had a difference (P < 0.05 ),the number of the difference over ±10% between the scores assesmented by trainers in their department and the scores assessed by nursing department had an obvious difference（P < 0.001）, The number of the shortage correlating clinical condition had an obvious difference（P < 0.001）.The number of failing in the exam before and after the teaching guidance had no differences（P > 0.05）. Conclusion: The management coach can effectively improve the effect of training under clinical settings

Light anti-nuclei production in pp collisions at s\sqrt{s}=7 and 14 TeV

A dynamically constrained coalescence model based on the phase space quantization and classical limit method was proposed to investigate the production of light nuclei (anti-nuclei) in non-single diffractive (NSD) pp collisions at $\sqrt{s}$=7 and 14 TeV. This calculation was based on the final hadronic state in the PYTHIA and PACIAE model simulations, the event sample consisted of 1.2$\times 10^8$ events in both simulations. The PACIAE model calculated $\bar D$ yield of 6.247$\times 10^{-5}$ in NSD pp collisions at $\sqrt{s}$=7 TeV is well comparing with the ALICE rough datum of 5.456$\times 10^{-5}$. It indicated the reliability of proposed method in some extent. The yield, transverse momentum distribution, and rapidity distribution of the $\bar D$, $^3{\bar{He}}$, and $_{\bar\Lambda} ^3{\bar H}$ in NSD pp collisions at $\sqrt{s}$=7 and 14 TeV were predicted by PACIAE and PYTHIA model simulations. The yield resulted from PACIAE model simulations is larger than the one from PYTHIA model. This might reflect the role played by the parton and hadron rescatterings.Comment: 5 pages, 2 figure

trans-Bis[acetone (2-hydroxy­benzo­yl)hydrazonato-κ2 N′,O]bis­(pyridine-κN)zinc(II)

In the title compound, [Zn(C10H11N2O2)2(C5H5N)2], the ZnII atom lies on an inversion centre, and is coordinated in a distorted octa­hedral geometry by two carbonyl O atoms and two imino N atoms from two anionic bidentate acetone (2-hydroxy­benzo­yl)hydrazone ligands and by two N atoms from two pyridine mol­ecules. The hydroxyl group acts as a donor, forming an intra­molecular O—H⋯N hydrogen bond

Acupuncture Deqi Intensity and Propagated Sensation along Channels May, Respectively, Differ due to Different Body Positions of Subjects

Acupuncture as an essential component of complementary and alternative medicine is gradually recognized and accepted by the mainstream of contemporary medicine. For obtaining preferable clinical effectiveness, Deqi is commonly regarded as efficacy predictor and parameter which is necessary to be achieved. Influential factors for acupuncture efficacy, like Deqi sensation as well as propagated sensation along channels (PSCs), enjoyed a long history in acupuncture basic research. Concerning this study, taking into account different positions on acupuncture Deqi sensation and PSCs, we would like to attest whether different body positions for subjects during needling procedure yield differed acupuncture Deqi sensation, particularly in terms of intensity, and PSCs. Methods. We used self-controlled method and selected 30 healthy subjects to perform needle insertion at Futu point (ST32) bilaterally. Then they were instructed to record the value of intensity of acupuncture sensation and the length and width of PSCs after removing the needle. Results. In regard to intensity of Deqi, kneeling seat position is stronger than supine position, accounting for 90% of the total number of subjects. In length of PSCs, kneeling seat position is greater than supine position, accounting for 56.7%. In width of PSCs, kneeling seat position is greater than supine position, accounting for 66.7%. Conclusion. Our findings show that needle inserting at Futu point (ST32) in kneeling seat position achieve better needle sensation and provide reference for clinical

Transcriptomic Response of Wolf Spider, \u3cem\u3ePardosa pseudoannulata\u3c/em\u3e, to Transgenic Rice Expressing \u3cem\u3eBacillus thuringiensis\u3c/em\u3e Cry1Ab Protein

Background: Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthetic insecticides. Non-target organisms can be exposed to Bt toxins through direct feeding or trophic interactions in the field. The wolf spider Pardosa pseudoannulata, one of the dominant predators in South China, plays a crucial role in the rice agroecosystem. In this study, we investigated transcriptome responses of the 5th instar spiders fed on preys maintained on Bt- and non-Bt rice. Results: Comparative transcriptome analysis resulted in 136 differentially expressed genes (DEGs) between spiderlings preying upon N. lugens fed on Bt- and non-Bt rice (Bt- and non-Bt spiderlings). Functional analysis indicated a potential impact of Bttoxin on the formation of new cuticles during molting. GO and KEGG enrichment analyses suggested that GO terms associated with chitin or cuticle, including “chitin binding”, “chitin metabolic process”, “chitin synthase activity”, “cuticle chitin biosynthetic process”, “cuticle hydrocarbon biosynthetic process”, and “structural constituent of cuticle”, and an array of amino acid metabolic pathways, including “alanine, asparatate and glutamate metabolism”, “glycine, serine and theronine metabolism”, “cysteine and methionine metabolism”, “tyrosine metabolism”, “phenylalanine metabolism and phenylalanine”, and “tyrosine and tryptophan biosynthesis” were significantly influenced in response to Cry1Ab. Conclusions: The Cry1Ab may have a negative impact on the formation of new cuticles during molting, which is contributed to the delayed development of spiderlings. To validate these transcriptomic responses, further examination at the translational level will be warranted

Enhanced pair production in multi-pulse trains electric fields with oscillation

For different alternating-sign multi-pulse trains electric fields with oscillation, the effects of the electric field pulse number and the relative phase of the combined electric field on pair production are investigated by solving quantum Vlasov equation. It is found that the number density of created particles in the combined electric fields is increased by more than one order of magnitude compared to the results without oscillating structure for both zero transverse momentum and full momentum space. In the case of zero transverse momentum, the created particles longitudinal momentum spectrum are monochromatic for large pulse numbers and some suitable relative phases. The number density depends nonlinearly on the relative phase that enables the optimal relative phase parameters for the number density. Moreover, for the full momentum space, the created particles number density and momentum spectrum under different multi-pulse trains electric fields are given and discussed. We also find that the number density as a function of pulse number satisfies the power law with index 5.342 for the strong but slowly varying electric field with large pulse numbers.Comment: 24 pages, 14 figure

Effects of florfenicol exposure during early life on toxicity, gut microbiota, and fecal metabolome in SD rats

Florfenicol (FLO) is a third-generation veterinary antibiotic with a high residue detection rate in food, which cause the toxicity of FLO even at low doses, receiving notable attention. The impact of FLO exposure during early life on health and gut microbiota is still unclear. Here, the effects of FLO exposure on toxicity, gut microbiota, drug resistance genes, and the fecal metabolome during early life were investigated in suckling Sprague-Dawley (SD) rats. The results showed that FLO exposure during early life significantly increased the body weight, and WBC and LY levels in the blood, induced inflammation in the liver and intestines. FLO had a dose-dependent effect on the alpha and beta diversity of the gut microbiota, increasing the ratio of Firmicutes to Bacteroides and the abundance of some pathogenic bacteria, and changing the abundance of bacteria related to energy metabolism and inflammation, also promoted the enrichment of drug resistance genes. The fecal metabolome also demonstrated the effect of FLO exposure on metabolic pathways related to energy metabolism and inflammation. In conclusion, this research shows that FLO exposure during early life can lead to excessive weight gain, an inflammatory response, gut microbiota imbalance, the enrichment of drug resistance genes, and effects on related metabolic pathways

Transcriptomic Response of Wolf Spider, \u3cem\u3ePardosa pseudoannulata\u3c/em\u3e, to Transgenic Rice Expressing \u3cem\u3eBacillus thuringiensis\u3c/em\u3e Cry1Ab Protein

Background: Bacillum thuringiensis (Bt) toxin produced in Cry1-expressing genetically modified rice (Bt rice) is highly effective to control lepidopteran pests, which reduces the needs for synthetic insecticides. Non-target organisms can be exposed to Bt toxins through direct feeding or trophic interactions in the field. The wolf spider Pardosa pseudoannulata, one of the dominant predators in South China, plays a crucial role in the rice agroecosystem. In this study, we investigated transcriptome responses of the 5th instar spiders fed on preys maintained on Bt- and non-Bt rice. Results: Comparative transcriptome analysis resulted in 136 differentially expressed genes (DEGs) between spiderlings preying upon N. lugens fed on Bt- and non-Bt rice (Bt- and non-Bt spiderlings). Functional analysis indicated a potential impact of Bttoxin on the formation of new cuticles during molting. GO and KEGG enrichment analyses suggested that GO terms associated with chitin or cuticle, including “chitin binding”, “chitin metabolic process”, “chitin synthase activity”, “cuticle chitin biosynthetic process”, “cuticle hydrocarbon biosynthetic process”, and “structural constituent of cuticle”, and an array of amino acid metabolic pathways, including “alanine, asparatate and glutamate metabolism”, “glycine, serine and theronine metabolism”, “cysteine and methionine metabolism”, “tyrosine metabolism”, “phenylalanine metabolism and phenylalanine”, and “tyrosine and tryptophan biosynthesis” were significantly influenced in response to Cry1Ab. Conclusions: The Cry1Ab may have a negative impact on the formation of new cuticles during molting, which is contributed to the delayed development of spiderlings. To validate these transcriptomic responses, further examination at the translational level will be warranted