The intersection among results in our study.

<p>According to the TNM staging and origin of tissues, CRC patients were divided into stages II, III, or IV colon or rectal cancer. There were 17 dysregulated miRNAs with a similar expression pattern in all 7 groups (II, III, IV, G1, G2, C, and R); 28 dysregulated miRNAs were found only in stage II and 51 only in stage III, but stage IV had 12 dysregulated miRNAs.</p

Identification of miRNAs Differentially Expressed in Clinical Stages of Human Colorectal Carcinoma—An Investigation in Guangzhou, China

<div><p>Aberrant expression of microRNAs (miRNAs) has been implicated in human cancer, including colorectal cancer (CRC). Such dysregulated miRNAs may have potential as diagnostic markers or therapeutic targets. However, the nature of an association between these miRNAs and clinical stages of CRC is still not clear. To this end, we performed a miRNA profiling of 1547 distinct human miRNAs using 31 samples of tumor and paired normal mucosa obtained from 31 CRC patients. Based on statistical analyses of profiling data, we identified 569 miRNAs that were significantly dysregulated in CRC relative to normal tissues (<i>P</i><0.05). Among the 569 dysregulated miRNAs, downregulation of 17 was associated with stages II, III, and IV colon and rectal cancers (separate or combined), according to our criteria. We also assessed the potential of these dysregulated miRNAs as diagnostic biomarkers for CRC patients who were without metastasis, and the value of the dysregulated miRNAs for predicting metastasis, lymph node and distant. Their distinct expression patterns in colon and rectal cancers were also examined. Although our findings cannot be immediately applied toward clinical diagnosis, our new study model for determining and assessing the biomarker potential of dysregulated miRNAs should be useful in further research in detection of human CRC.</p></div

Evaluation of the potential of miR-374a, miR-4634, miR-516a-5p as diagnostic biomarkers in clinical application.

<p>Among them, miR-374a was dysregulated in stage II, miR-4634 was related to lymph node metastasis in stage III, and miR-516a-5p might be associated with distant metastasis.</p

Expression levels of dysregulated miRNAs.

<p>Box plots of expression levels of six miRNAs in both tumors and paired normal tissues. The relative level of the six miRNAs was normalized to internal control gene and was showed as normalized CT. The line represents the median value—the higher the value, the lower the expression level. Paired t-tests were performed to examine the differences in miRNAs between tumors and the paired normal tissues.</p

ROC curve analysis of 17 dysregulated miRNAs.

<p>Analysis of data implied that most of the dysregulated miRNAs have potential as diagnostic biomarkers for CRC detection, with high sensitivity and specificity.</p

Clinical and histopathological characteristics of patients.

<p>* Information not available.</p

Expression levels of miR-145*, -30e*, -378*, -125a-5p, -3195, and -4770 in an independent sample test.

<p>Expression levels of miR-145*, -30e*, -378*, -125a-5p, -3195, and -4770 in an independent sample test.</p

Additional file 1 of Hemodynamics combined with inflammatory indicators exploring relationships between ischemic stroke and symptomatic middle cerebral artery atherosclerotic stenosis

Additional file 1: Supplementary Materials

Population-based study of chlamydial and gonococcal infections among women in Shenzhen, China: Implications for programme planning

<div><p>This study was aimed to estimate the prevalences of chlamydia (CT) and gonococcal (NG) infections and explore risk factors associated with the CT infection among women in Shenzhen, China. We collected socio-demographic and clinical data from women (aged 20–60) and determined positivity of CT or NG by nucleic acid amplification test (NAAT) with self-collected urine specimens. We estimated prevalence of CT and NG and determined risk factors associated with CT infection. Among 9,207 participants, 4.12% (95% confidence interval [CI], 3.71%-4.53%) tested positive for CT and 0.17% (95% CIs, 0.09%-0.25%) for NG. Factors significantly associated with CT infection included being an ethnic minority (ethnicity other than Han China) (Adjusted odds ratio [AOR], 1.9; 95% CI, 1.2–3.0), using methods other than condom for contraception (AOR, 1.5; 95% CI, 1.2–1.8), having a history of adverse pregnancy outcomes (AOR, 1.4; 95% CI, 1.1–1.8), and experiencing reproductive tract symptoms in the past three months (AOR, 1.3; 95% CI, 1.0–1.7). we found that CT infection is prevalent among women in Shenzhen, China and associated with both demographic and behavioral factors. A comprehensive CT screening, surveillance and treatment programme targeting this population is warranted.</p></div

Factors associated with positivity of <i>C</i>. <i>trachomatis</i>: univariate and multivariate logistic regression analyses.

<p>Factors associated with positivity of <i>C</i>. <i>trachomatis</i>: univariate and multivariate logistic regression analyses.</p