Wireless sensor integration for bridge model health monitoring

An integrated hardware and software system for a scalable wireless sensor network (WSN) is designed and developed for structural health monitoring. An extension sensor board is designed, developed, and calibrated to meet the requirements for structural vibration monitoring and modal identification. The extension sensor board has 3 axes of accelerometers in three directions and a temperature sensor. Software components have been implemented within the TinyOS operating system to provide a flexible software platform and scalable performance for structural health monitoring applications. The prototype WSN is deployed on a reduced-scale bridge model with 3 nodes in a single-hop network for performing dynamic monitoring civil engineering structures. Two output-only time-domain system identification methods are employed namely, the Frequency Domain Decomposition (FDD) method and the Natural Excitation Technique (NExT) combined with the Eigensystem Realization Algorithm (ERA). Testing results show that the WSN provides accurate vibration data for identifying vibration modes of a bridge

Three-Dimensional Modeling of Tea-Shoots Using Images and Models

In this paper, a method for three-dimensional modeling of tea-shoots with images and calculation models is introduced. The process is as follows: the tea shoots are photographed with a camera, color space conversion is conducted, using an improved algorithm that is based on color and regional growth to divide the tea shoots in the images, and the edges of the tea shoots extracted with the help of edge detection; after that, using the divided tea-shoot images, the three-dimensional coordinates of the tea shoots are worked out and the feature parameters extracted, matching and calculation conducted according to the model database, and finally the three-dimensional modeling of tea-shoots is completed. According to the experimental results, this method can avoid a lot of calculations and has better visual effects and, moreover, performs better in recovering the three-dimensional information of the tea shoots, thereby providing a new method for monitoring the growth of and non-destructive testing of tea shoots

Expression and Functional Analysis of the BCL2-Associated Agonist of Cell Death (BAD) Gene in the Sheep Ovary During the Reproductive Cycle

Most ewes in China are seasonally polyestrous with normal ovulatory cycles, which is controlled by photoperiod (length of the daily light phase). These ewes are estrous in the short-day season and anestrus in the long-day season and cannot mate during anestrus. Thus seasonal breeding limits both diversification and intensification of production. If sheep can estrus all round year, it can be mated twice per year, which can greatly improve the economic benefits. To change seasonal estrus at the genetic level and cultivating new sheep breeds, it is important to understand the molecular mechanisms of seasonal breeding trait in sheep. The BCL2-associated agonist of cell death (BAD) gene being a regulator of cellular apoptosis was identified by our previous RNA-Seq, which is associated with follicular development in mammalian ovaries. However, the mechanism how BAD can regulate estrus in sheep was poorly understood. In this study, we characterized ovine BAD, including full-length mRNA cloning and protein sequence prediction, as well as BAD expression profile in Small-tailed Han (STH) sheep. The highest expression levels of BAD were observed in sheep hypothalamus, lung, and pituitary, while the lowest expression was in liver. Functional analysis of BAD was performed in primary granulosa cells of sheep. The concentration of P4 was significantly increased after RNAi interference of BAD, while P4 level was shown to be opposite after BAD overexpression in vitro. It has been found that BAD can reduce progesterone levels by promoting ovarian GC apoptosis, which might be involved in regulating the estrus cycle in sheep

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Stereo Educational Game with Vision Based Interaction in Virtual Environment

International audienceInteraction and immersion are crucial to educational game quality. Thus, a vision based marker location interaction and stereo rendering method for the game are proposed. This approach could support the players to have more intuitive interaction and more immersion feeling. First, a general architecture of the educational game based on computer vision marker localization interaction was presented. Then, a new method to vision based marker localization and identification interaction was studied. The basic idea of this interaction is using a static camera to track the position and pose information of the marker in the handheld device. So the game system can judge the operation purposes of players such as pick up or put down a virtual objects based on the position and pose information of the marker easily. The experiment result shows this interactive method has a high tracking accuracy. Furthermore, rendering module of the game is designed by using a stereo rendering method, so it can produce stereo vision for players. Last, a feeding animal game for children is realized to verify the presented approaches. Children can carry different food to different animals by a handheld device with marker so that they can learn what food the animals like. The running result illustrates that the presented approaches are effective, and they can provide a natural interaction for game in virtual environmen

Impact of the pre-chamber nozzle orifice configurations on combustion and performance of a Natural Gas Engine

In this study, a pre-chamber was designed to form near stoichiometric mixture and provide multiple turbulent flame jets to ignite the lean mixture and accelerate the combustion in the main combustion chamber for a natural gas engine. A CFD simulation was employed to investigate the impact of the pre-chamber nozzle configurations on flow and combustion processes inside the engine, as well as on the performance of the engine. Various configurations were investigated, including orifice number of 4 to 8 and orifice diameter ranging from 1.6 m to 2.9 mm. A non-dimensional parameter, β, was used to characterize the relative flow area of these configurations. The numerical results indicate that, for a given nozzle flow area, among the design of different orifice numbers, the 6-orifice design can obtain the optimal combustion and engine performance. Otherwise, a design of more orifices leads to slower flame penetrating speed in the main-chamber, and the design of less orifices leads to slower circumferential flames propagations in the main-chamber. Moreover, for a 6-orifice pre-chamber, the optimal orifice diameter was found to be 2.0 mm, corresponding to a β value of 0.3. A design of larger diameters leads to slower penetrating for the flame jets and insufficient radial flame propagations in the main-chamber, while a design of relatively smaller orifice diameters leads to insufficient circumferential flames propagations in the main-chamber. Additionally, for the engine performance, all the pre-chamber designs improve the indicated efficiency and reduce the NOx emission. Especially, the design of 6-orifice with diameter of 2.0 mm achieves a 35.0% increase of indicated thermal efficiency and a 78.0% reduction of NOx emission compared to the prototype engine