The effects of rs199618935 on PTPN11 transcriptional activity as determined by luciferase reporter assay.

<p>In three tested cell lines, significant difference was observed for the relative luciferase activity among allele 12, 14 and 15 constructs. * indicates <i>P</i><0.05, ** indicates <i>P</i><0.01 compared with construct containing allele 12 within the same group.</p

Example sequencing and genotyping output for rs199618935.

<p>The upper panel showed an example of the genotyping assay results. DNA was run in 7% non-denaturing polyacrylamide gel electrophoresis (PAGE) and visualized by silver staining (the genotypes from lane 1 to 11 was 15/16, 14/16, 14/15, 12/13, 12/14, 12/12, 14/14, 15/15, 12/15, 11/15 and 11/12, respectively). The lower panel displayed the sequences of the most three common alleles (allele 12, 14 and 15). The underlined base-pairs indicated the “TCA” repeat polymorphism.</p

Expression of PTPN11 in HCC tissues and cell lines with different genotypes.

<p>(A) PTPN11 mRNA expression (mean ±SEM.) in HCC tissue and adjacent non-HCC tissues samples, by rs199618935 genotype. N = 39 for genotype 12–12, N = 5 for genotype 14–14, N = 4 for genotype 14–15. ** indicates <i>P</i><0.01 compared with 12–12 genotype within the same group. (B) PTPN11 mRNA expression (mean ±SD) in cell lines with different rs199618935 genotypes. * indicates <i>P</i><0.05, ** indicates <i>P</i><0.01 compared with Hep-G2 carrying one 12 allele.</p

Logistic regression analyses for the association between rs199618935 and risk of HCC in HBV positive and negative groups.

a<p>Adjusted for sex, age, smoking status, drinking status and HBV infection.</p><p>Logistic regression analyses for the association between rs199618935 and risk of HCC in HBV positive and negative groups.</p

Influence of rs199618935 on PTPN11 mRNA local folding structures.

<p>The local structure changes were illustrated by RNAfold using default parameters.</p

Relative PTPN11 expression in HCC tumor tissues <i>vs.</i> adjacent non-tumor tissues.

<p>** indicates <i>P</i><0.01 compared with paired HCC tumor tissue. N = 48.</p

Demographic characteristics among HCC cases and controls.

<p>Cases indicate patients with HCC and controls are non-cancerous patients.</p>a<p>Two-sided two-sample <i>t</i>-test between cases and controls.</p>b<p>χ² test for differences between cases and controls.</p><p>Demographic characteristics among HCC cases and controls.</p