NMR Characterization of PAMAM_G5.NH₂ Entrapped Atomic and Molecular Assemblies

High resolution NMR spectroscopy, NMR diffusiometry, and NMR cryoporometry have been used to investigate aqueous solution (D₂O) of PAMAM_G5.NH₂-(Au)_25–100 and PAMAM_G5.NH₂-(H₂O)₁₀₀₀–(H₂O)₄₀₀₀ systems. In the case of dendrimer entrapped gold nanoparticles, the detailed analysis of high resolution NMR spectra has shown that no precursor complex formation happens under the circumstances applied for reduction. Further PGSE results verify that gold nanoparticles of 1.9–2.6 nm size are entrapped in the outermost part of the dendrimers and probably more than one dendrimer molecule takes part in the stabilization process. This system looks like a transition state between dendrimer encapsulated nanoparticles (DENs) and dendrimer stabilized nanoparticles (DSNs), and we deal with it in details for what this means. NMR cryoporometry experiments were performed to detect the encapsulation of water molecules. The results show that, in the swelling PAMAM_G5.NH₂ dendrimers, by adding water step by step, there are specific cavities for water with diameters of 3.6 and 5.2 nm. These cavities have a penetrable wall for water molecules and probably exist very close to the terminal groups. The permeability of the cavities is increasing with the increase of the water content. In dilute solution, the formation of nanoparticles is determined by the ratio of the rate of nucleation and aggregation and the latter is affected by the PAMAM_G5.NH₂

Partitioned Image Data for Machine Learning Analysis of Molecular Biology Figures

<p><strong> Corpus Composition</strong></p> <p>This data collection provides four types of hand-curated images from open access research articles images. The types are:</p> <ol> <li>chart (n=811): data displays such as bar charts, scatterplots, line graphs, etc.</li> <li>diagram (n=816): any general conceptual diagram</li> <li>gel (n=811): the output of electrophoresis experiments in Northern, Western, or Southern Blot experiments. </li> <li>histology (n=816): microscope images of tissue  wih histological staining</li> </ol> <p>The images are simply organized in subdirectories as individual files. File names are based on PubMed Id and Figure number. </p

Dendrimer-Modified MoS₂ Nanoflakes as a Platform for Combinational Gene Silencing and Photothermal Therapy of Tumors

Exploitation of novel hybrid nanomaterials for combinational tumor therapy is challenging. In this work, we synthesized dendrimer-modified MoS₂ nanoflakes for combinational gene silencing and photothermal therapy (PTT) of cancer cells. Hydrothermally synthesized MoS₂ nanoflakes were modified with generation 5 (G5) poly­(amidoamine) dendrimers partially functionalized with lipoic acid via disulfide bond. The formed G5-MoS₂ nanoflakes display good colloidal stability and superior photothermal conversion efficiency and photothermal stability. With the dendrimer surface amines on their surface, the G5-MoS₂ nanoflakes are capable of delivering Bcl-2 (B-cell lymphoma-2) siRNA to cancer cells (4T1 cells, a mouse breast cancer cells) with excellent transfection efficiency, inducing 47.3% of Bcl-2 protein expression inhibition. <i>In vitro</i> cell viability assay data show that cells treated with the G5-MoS₂/Bcl-2 siRNA polyplexes under laser irradiation have a viability of 21.0%, which is much lower than other groups of single mode PTT treatment (45.8%) or single mode of gene therapy (68.7%). Moreover, the super efficacy of combinational therapy was further demonstrated by treating a xenografted 4T1 tumor model <i>in vivo</i>. These results suggest that the synthesized G5-MoS₂ nanoflakes may be employed as a potential nanoplatform for combinational gene silencing and PTT of tumors

Partitioned Image Data for Machine Learning Analysis of Molecular Biology Figures

<p><strong> Corpus Composition</strong></p> <p>This data collection provides four types of hand-curated images from open access research articles images. The types are:</p> <ol> <li>chart (n=811): data displays such as bar charts, scatterplots, line graphs, etc.</li> <li>diagram (n=816): any general conceptual diagram</li> <li>gel (n=1182): the output of electrophoresis experiments in Northern, Western, or Southern Blot experiments. </li> <li>histology (n=3458): microscope images of tissue with histological staining</li> </ol> <p>The images are simply organized in subdirectories as individual files. File names are based on PubMed Id and Figure number. </p

Multifunctional Lactobionic Acid-Modified Dendrimers for Targeted Drug Delivery to Liver Cancer Cells: Investigating the Role Played by PEG Spacer

We report the development of a lactobionic acid (LA)-modified multifunctional dendrimer-based carrier system for targeted therapy of liver cancer cells overexpressing asialoglycoprotein receptors. In this study, generation 5 (G5) poly­(amidoamine) (PAMAM) dendrimers were sequentially modified with fluorescein isothiocyanate (FI) and LA (or polyethylene glycol (PEG)-linked LA, PEG-LA), followed by acetylation of the remaining dendrimer terminal amines. The synthesized G5.NHAc-FI-LA or G5.NHAc-FI-PEG-LA conjugates (NHAc denotes acetamide groups) were used to encapsulate a model anticancer drug doxorubicin (DOX). We show that both conjugates are able to encapsulate approximately 5.0 DOX molecules within each dendrimer and the formed dendrimer/DOX complexes are stable under different pH conditions and different aqueous media. The G5.NHAc-FI-PEG-LA conjugate appears to have a better cytocompatibility, enables a slightly faster DOX release rate, and displays better liver cancer cell targeting ability than the G5.NHAc-FI-LA conjugate without PEG under similar experimental conditions. Importantly, the developed G5.NHAc-FI-PEG-LA/DOX complexes are able to specifically inhibit the growth of the target cells with a better efficiency than the G5.NHAc-FI-LA/DOX complexes at a relatively high DOX concentration. Our results suggest a key role played by the PEG spacer that affords the dendrimer platform with enhanced targeting and therapeutic efficacy of cancer cells. The developed LA-modified multifunctional dendrimer conjugate with a PEG spacer may be used as a delivery system for targeted liver cancer therapy and offers new opportunities in the design of multifunctional drug carriers for targeted cancer therapy applications

Acetylated Polyethylenimine-Entrapped Gold Nanoparticles Enable Negative Computed Tomography Imaging of Orthotopic Hepatic Carcinoma

Developing an effective computed tomography (CT) contrast agent is still a challenging task for precise diagnosis of hepatic carcinoma (HCC). Here, we present the use of acetylated polyethylenimine (PEI)-entrapped gold nanoparticles (Ac-PE-AuNPs) without antifouling modification for negative CT imaging of HCC. PEI was first linked to fluorescein isothiocyanate (FI) and then utilized as a vehicle for the entrapment of AuNPs. The particles were then acetylated to reduce its positive surface potential. The designed Ac-PE-AuNPs were characterized by various techniques. We find that the Ac-PE-AuNPs with a uniform size distribution (mean diameter = 2.3 nm) are colloidally stable and possess low toxicity in the studied range of concentration. Owing to the fact that the particles without additional antifouling modification were mainly gathered in liver, the Ac-PE-AuNPs could greatly improve the CT contrast enhancement of normal liver, whereas poor CT contrast enhancement appeared in liver necrosis region caused by HCC. As a result, HCC could be easily and precisely diagnosed. The designed Ac-PE-AuNPs were demonstrated to have biocompatibility through in vivo biodistribution and histological studies, hence holding an enormous potential to be adopted as an effective negative CT contrast agent for diagnosis of hepatoma carcinoma

A Microfluidic Chip Integrated with Hyaluronic Acid-Functionalized Electrospun Chitosan Nanofibers for Specific Capture and Nondestructive Release of CD44-Overexpressing Circulating Tumor Cells

Detection of circulating tumor cells (CTCs) in peripheral blood is of paramount significance for early-stage cancer diagnosis, estimation of cancer development, and individualized cancer therapy. Herein, we report the development of hyaluronic acid (HA)-functionalized electrospun chitosan nanofiber (CNF)-integrated microfludic platform for highly specific capture and nondestructive release of CTCs. First, electrospun CNFs were formed and modified with zwitterion of carboxyl betaine acrylamide (CBAA) via Michael addition reaction and then targeting ligand HA through a disulfide bond. We show that the formed nanofibers still maintain the smooth fibrous morphology after sequential surface modifications, have a good hemocompatibility, and exhibit an excellent antifouling property due to the CBAA modification. After being embedded within a microfluidic chip, the fibrous mat can capture cancer cells (A549, a human lung cancer cell line) with an efficiency of 91% at a flow rate of 1.0 mL/h. Additionally, intact release of cancer cells is able to be achieved after treatment with glutathione for 40 min to have a release efficiency of 90%. Clinical applications show that 9 of 10 nonsmall-cell lung cancer patients and 5 of 5 breast cancer patients are diagnosed to have CTCs (1 to 18 CTCs per mL of blood). Our results suggest that the developed microfluidic system integrated with functionalized CNF mats may be employed for effective CTCs capture for clinical diagnosis of cancer

Construction of Hybrid Alginate Nanogels Loaded with Manganese Oxide Nanoparticles for Enhanced Tumor Magnetic Resonance Imaging

Development of sensitive contrast agents for positive magnetic resonance (MR) imaging of biosystems still remains a great challenge. Herein, we report a facile process to construct hybrid alginate (AG) nanogels (NGs) loaded with manganese oxide (Mn₃O₄) nanoparticles (NPs) for enhanced tumor MR imaging. The obtained AG/PEI-Mn₃O₄ NGs with a mean size of 141.6 nm display excellent colloidal stability in aqueous solution and good cytocompatibility in the studied concentration range. Moreover, the hybrid NGs have a high <i>r</i>₁ relaxivity of 26.12 mM^–1 s^–1, which is about 19.5 times higher than that of PEI-Mn₃O₄ NPs with PEI surface amine acetylated (PEI.Ac–Mn₃O₄ NPs). Furthermore, the AG/PEI-Mn₃O₄ NGs presented longer blood circulation time and better tumor MR imaging performances <i>in vivo</i> than PEI.Ac–Mn₃O₄ NPs. With the good biosafety confirmed by histological examinations, the developed AG/PEI-Mn₃O₄ NGs may be potentially used as an efficient contrast agent for enhanced MR imaging of different biosystems

T₂ signal intensity value of the wall of ectopic endometriotic lesions at different time points.

<p>T₂ signal intensity value of the wall of ectopic endometriotic lesions at different time points.</p

The surgically induced ectopic endometriotic lesions at four weeks post operation.

<p>The tubal cystic structure with a size of 23.8×4.7×3.9 mm was noticed at the fixed site (long arrow). The ectopic lesions were full of liquids and the small dendritic vessels on the surface of the wall were also clearly observed. Note that a strip of the adhesion tissues (arrowhead) was also observed between the ectopic lesions and abdominal wall.</p