1 research outputs found
Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens
Enzymatic
synthesis of all 15 naturally occurring human ABH antigens
was achieved using a diversity-oriented enzymatic modular assembly
(EMA) strategy. Three enzyme modules were developed, each one-pot
multienzyme module comprises a glycosyltransferase and one or two
corresponding sugar nucleotide generating enzyme(s). These multienzyme
cascade processes provide an efficient and convenient platform for
collective synthesis of all 15 ABH antigens in three operationally
simple steps from five readily available disaccharide acceptors and
three simple free sugars as donor precursors