1 research outputs found
In Vivo Photoacoustic Detection and Imaging of Peroxynitrite
Photoacoustic
detection is an emerging noninvasive and nonionizing
detection technique with the merits of rich contrast, high resolution,
and deep tissue penetration, especially for in vivo detection and
imaging. Herein, we developed a photoacoustic (PA) molecular imaging
probe (denoted as nanonaps) composed of a naphthalocyanine dye and
a heptamethine dye as the internal standard with unchanged signals
at 860 nm and the sensing component with peroxynitrite (ONOO<sup>โ</sup>) target-decreased signals at 775 nm, respectively. The as-prepared
nanonaps displayed high sensitivity and specificity of ONOO<sup>โ</sup> both in vitro and in vivo. The PA<sub>860</sub>/PA<sub>775</sub> ratio was increased as a function of the concentration of ONOO<sup>โ</sup> (0โ250 nM). More interestingly, our ratiometric
nanonaps could be used for in vivo detection and imaging of ONOO<sup>โ</sup>