Raw sequencing data of Anaplamsa phagocytophilum loci (ankA, msp4, groEL) obtained from 454 and parameter files to clean these data using MOTHUR

<p>A compressed archive including: i) raw sequences in ssf file; ii) Mothur oligo files to sort out sequences among loci and individual samples.</p

Impact of a range of <i>p</i>CO₂ on: A. number of eggs produced per female; B. number of eggs per cocoon; C. number of cocoon produced per female (experiment 4).

<p>The number of eggs produced per female was 3 times higher in high compared to low <i>p</i>CO₂ (27 k vs 0.4 k µatm) and a significant logarithmic relationship was observed between the two parameters (F = 8.36, p<0.016; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029568#pone-0029568-g003" target="_blank">Figure 3A</a>). This was the consequence of an increased number of cocoon produced per female (significant logarithmic relationship, F = 7.79, p<0.02; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029568#pone-0029568-g003" target="_blank">Figure 3B</a>) with no effect on the number of eggs per cocoon (non significant logarithmic relationship, F = 0.43, p<0.53; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029568#pone-0029568-g003" target="_blank">Figure 3C</a>).</p

Summary of the impact of low <i>p</i>CO₂ (27063 µatm) on <i>S. roscoffensis</i> life cycle.

<p>Summary of the impact of low <i>p</i>CO₂ (27063 µatm) on <i>S. roscoffensis</i> life cycle.</p

<i>Symsagittifera roscoffensis</i> after a 24 h exposure to seawater with different pH (experiment 5).

<p>Animals in the lower pH started to bleach.</p

Impact of a range of <i>p</i>CO₂ on the growth rate of newly hatched juveniles in presence of their photosymbiotic algae (9 days exposure; Experiment 3).

<p>A significant logarithmic relationships was observed between <i>p</i>CO₂ and juvenile growth rate (F = 7.84, p<0.019), the growth rate being 19% faster in the highest test <i>p</i>CO₂ (27 k µatm) compared to the lowest (0.4 k µatm).</p

Visual representation of patterns of genotype group associations found in this study.

<p>The graph includes genotypes of both target loci and from all three host species. Each distinct community of co-occurring genotype groups is highlighted in a different color, and the thickness of lines is proportional to the frequency of co-occurrence. Vertices were placed empirically; genotypes located towards the top of the figure correspond to those mainly found in bank voles, while those towards the bottom were associated with chipmunks. The cluster of genotypes at the bottom left corresponds to strains of <i>B. burgdorferi</i> s.s. and that on the top and bottom right to <i>B. afzelii</i>.</p

Results of the AMOVA performed on <i>rplB</i> sequence data.

a<p>degrees of freedom; ^bsums of squares; ^cmean squares; ^dcomponents of covariance; ^epercentage contribution to the total covariance; ^fPhi statistic.</p

Results of the AMOVA performed on <i>ospC</i> sequence data.

<p>Results of the AMOVA performed on <i>ospC</i> sequence data.</p

Phylogenetic network of <i>rplB</i> sequences.

<p>The network includes consensus sequences of the genotypes recovered in this study (blue) as well as reference sequences for species in the <i>Borrelia burgdorferi</i> complex. The genotypes identified in this study formed empirically delineated genotype groups that are indicated with blue dotted lines. The pie charts connected to each main genotype group show the proportion of genotypes in the group that were isolated from each host species: Siberian chipmunks (purple), bank voles (green), or wood mice (orange).</p

Percentage (%) of host individuals of each host species in which co-infection by at least two genotypes of the <i>Borrelia burgdorferi</i> species complex was detected.

<p>Results are shown for both the <i>rplB</i> and <i>ospC</i> datasets.</p