Fire Resistance of Ultra-High Strength Concrete Filled Steel Tubular Columns

Ph.DDOCTOR OF PHILOSOPH

Buckling Resistance of Axially Restrained Chord Members of Grid Structure at Elevated Temperatures

This paper investigates the behavior of large span grid structure exposed to a localized fire. The localized fire may generate hot smoke and thus induce non-uniform temperature distribution in the grid structure. The thermal expansion of the heated members tend to be axially restrained by the adjacent cold members thus inducing additional forces on the critical members of the grid structure. The buckling resistance of axially restrained member at elevated temperature may be obtained based on second order analysis of member with initial lateral imperfection by considering force equilibrium at deformed geometry and cross section resistance being reached. The critical temperature of the member is reached when the axial force reaches its buckling resistance. It is found that the critical temperature of members with initial lateral imperfection was higher than that without such imperfection for chord members with large slenderness ratio and high axial restraint

Prediction of fire resistance of concrete encased steel composite columns using artificial neural network

https://doi.org/10.1016/j.engstruct.2021.112877Engineering structures24

Fire performance of composite columns made of high strength steel and concrete

10.1016/j.jcsr.2021.106640Journal of Constructional Steel Researc

Structural behaviour of double skin composite system using ultra-lightweight cement composite

10.1016/j.conbuildmat.2015.03.092Construction and Building Materials8651-6

S100A6 inhibits MDM2 to suppress breast cancer growth and enhance sensitivity to chemotherapy

Abstract Background S100A6 and murine double minute 2 (MDM2) are important cancer-related molecules. A previous study identified an interaction between S100A6 and MDM2 by size exclusion chromatography and surface plasmon resonance experiments. The present study investigated whether S100A6 could bind to MDM2 in vivo and further explored its functional implication. Methods Co-immunoprecipitation, glutathione-S-transferase pull-down assay, and immunofluorescence were performed to determine the in vivo interaction between S100A6 and MDM2. Cycloheximide pulse-chase assay and ubiquitination assay were performed to clarify the mechanism by which S100A6 downregulated MDM2. In addition, clonogenic assay, WST-1 assay, and flow cytometry of apoptosis and the cell cycle were performed and a xenograft model was established to evaluate the effects of the S100A6/MDM2 interaction on growth and paclitaxel-induced chemosensitivity of breast cancer. The expressions of S100A6 and MDM2 in patients with invasive breast cancer were analyzed by immunohistochemistry. In addition, the correlation between the expression of S100A6 and the response to neoadjuvant chemotherapy was statistically analyzed. Results S100A6 promoted the MDM2 translocation from nucleus to cytoplasm, in which the S100A6 bound to the binding site of the herpesvirus-associated ubiquitin-specific protease (HAUSP) in MDM2, disrupted the MDM2–HAUSP–DAXX interactions, and induced the MDM2 self-ubiquitination and degradation. Furthermore, the S100A6-mediated MDM2 degradation suppressed the growth of breast cancer and enhanced its sensitivity to paclitaxel both in vitro and in vivo. For patients with invasive breast cancer who received epirubicin and cyclophosphamide followed by docetaxel (EC-T), expressions of S100A6 and MDM2 were negatively correlated, and high expression of S100A6 suggested a higher rate of pathologic complete response (pCR). Univariate and multivariate analyses showed that the high expression of S100A6 was an independent predictor of pCR. Conclusion These results reveal a novel function for S100A6 in downregulating MDM2, which directly enhances sensitivity to chemotherapy

WAFER-LEVEL MEMS PACKAGE BY GOLD-TIN BONDING METHOD

ABSTRACT This paper reports some results for an on-going program in wafer-level MEMS package Institute of Microsystems at Huazhong University of Science and Technology. The final goal was to come up with a method usable for various types of MEMS devices in wafer level so that the low cost and high reliability can be achieved at the same time. In this particular paper, three closed-loop microheaters of 5µm, 7µm and 9µm width were designed. By reactive ion sputtering technique, two classes of samples are presented. The first one was first cosputtered with Ni/Cr and then sputtered with Au metal as heating material, the second one was sputtered with Cr, Sn and Au respectively as heating material. The bonding of the former sample based on the Ni/Cr and Au heating material failed. The eutectic bonding experiment of the later sample based on the Cr, Sn and Au heating material by global heating method was completed in annealing oven at temperature of about 400°C for 20 minutes and by local heating method was applyed current of 0.8 A into the microheater. The SEM testing result showed the eutectic bonding of Au-Sn by global heating was successful. More results will be reported during the conference presentation

Chronic fatigue syndrome patients have alterations in their oral microbiome composition and function.

Host-microbe interactions have been implicated in the pathogenesis of chronic fatigue syndrome (CFS), but whether the oral microbiome is altered in CFS patients is unknown. We explored alterations of the oral microbiome in Chinese Han CFS patients using 16S rRNA gene sequencing and alterations in the functional potential of the oral microbiome using PICRUSt. We found that Shannon and Simpson diversity indices were not different in CFS patients compared to healthy controls, but the overall oral microbiome composition was different (MANOVA, p < 0.01). CFS patients had a higher relative abundance of Fusobacteria compared with healthy controls. Further, the genera Leptotrichia, Prevotella, and Fusobacterium were enriched and Haemophilus, Veillonella, and Porphyromonas were depleted in CFS patients compared to healthy controls. Functional analysis from inferred metagenomes showed that bacterial genera altered in CFS patients were primarily associated with amino acid and energy metabolism. Our findings demonstrate that the oral microbiome in CFS patients is different from healthy controls, and these differences lead to shifts in functional pathways with implications for CFS pathogenesis. These findings increase our understanding of the relationship between the oral microbiota and CFS, which will advance our understanding of CFS pathogenesis and may contribute to future improvements in treatment and diagnosis