Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: wet lab procedure

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.Molecular basis of virus replication, viral pathogenesis and antiviral strategie

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for i

Herbage and milk productivity and quality when grazing different legumes and herbs

In order to evaluate how different legumes and herbs affects the quality of organic milk, a grazing study was performed at the organic research station Rugballegaard with 48 dairy cows. Swards with Lucerne (Medicargo sativa), red clover (Trifolium pratense), white clover (Trifolium repens) and white clover together with chicory (Cichorium intybus), respectively, were established in 2005 together with perennial ryegrass (Lolium perenne) in two replicates. The study was carried out in 2006 over three two-weeks periods during the season. Generally, the proportion of legumes and chicory was high in the four species mixtures. Sward composition highly affected grazing behaviour. Sward height measurements indicate that the white clover mixture was eaten without much selection, the red clover was partly avoided in some areas, and in contrast lucerne seemed to be eaten selectively. In chicory swards selection occurred in a patchy way