Induction of donor-specific transplantation tolerance to skin and cardiac allografts using mixed chimerism in (A + B → A) in rats

Mixed allogeneic chimerism (A + B → A) was induced in rats by reconstitution of lethally irradiated LEW recipients with a mixture of T-cell depleted (TCD) syngeneic and TCD allogeneic ACI bone marrow. Thirty-seven percent of animals repopulated as stable mixed lymphopoietic chimeras, while the remainder had no detectable allogeneic chimerism. When evaluated for evidence of donor-specific transplantation tolerance, only those recipients with detectable allogeneic lymphoid chimerism exhibited acceptance of donor-specific skin and cardiac allografts. Despite transplantation over a major histocompatibility complex (MHC)- and minor-disparate barrier, animals accepted donor-specific ACI skin and primarily vascularized cardiac allografts permanently, while rejecting third party Brown Norway (BN) grafts. The tolerance induced was also donor-specific in vitro as evidenced by specific hyporeactivity to the allogeneic donor lymphoid elements, yet normal reactivity to MHC-disparate third party rat lymphoid cells. This model for mixed chimerism in the rat will be advantageous to investigate specific transplantation tolerance to primarily vascularized solid organ grafts that can be performed with relative ease in the rat, but not in the mouse, and may provide a method to study the potential existence of organ- or tissue-specific alloantigens in primarily vascularized solid organ allografts. © 1993

Shortcuts through Colocation Facilities

Network overlays, running on top of the existing Internet substrate, are of perennial value to Internet end-users in the context of, e.g., real-time applications. Such overlays can employ traffic relays to yield path latencies lower than the direct paths, a phenomenon known as Triangle Inequality Violation (TIV). Past studies identify the opportunities of reducing latency using TIVs. However, they do not investigate the gains of strategically selecting relays in Colocation Facilities (Colos). In this work, we answer the following questions: (i) how Colo-hosted relays compare with other relays as well as with the direct Internet, in terms of latency (RTT) reductions; (ii) what are the best locations for placing the relays to yield these reductions. To this end, we conduct a large-scale one-month measurement of inter-domain paths between RIPE Atlas (RA) nodes as endpoints, located at eyeball networks. We employ as relays Planetlab nodes, other RA nodes, and machines in Colos. We examine the RTTs of the overlay paths obtained via the selected relays, as well as the direct paths. We find that Colo-based relays perform the best and can achieve latency reductions against direct paths, ranging from a few to 100s of milliseconds, in 76% of the total cases; 75% (58% of total cases) of these reductions require only 10 relays in 6 large Colos.Comment: In Proceedings of the ACM Internet Measurement Conference (IMC '17), London, GB, 201