22 research outputs found
Extension of human lncRNA transcripts by RACE coupled with long-read high-throughput sequencing (RACE-Seq)
Long non-coding RNAs (lncRNAs) constitute a large, yet mostly uncharacterized fraction of the mammalian transcriptome. Such characterization requires a comprehensive, high-quality annotation of their gene structure and boundaries, which is currently lacking. Here we describe RACE-Seq, an experimental workflow designed to address this based on RACE (rapid amplification of cDNA ends) and long-read RNA sequencing. We apply RACE-Seq to 398 human lncRNA genes in seven tissues, leading to the discovery of 2,556 on-target, novel transcripts. About 60% of the targeted loci are extended in either 5′ or 3′, often reaching genomic hallmarks of gene boundaries. Analysis of the novel transcripts suggests that lncRNAs are as long, have as many exons and undergo as much alternative splicing as protein-coding genes, contrary to current assumptions. Overall, we show that RACE-Seq is an effective tool to annotate an organism’s deep transcriptome, and compares favourably to other targeted sequencing techniques
Training of potential program implementers for the Tin Ka Ping P.A.T.H.S. Project in China : subjective outcome evaluation findings
202207 bcchVersion of RecordPublishe
Tin Ka Ping P.A.T.H.S. Project in Mainland China : preliminary evaluation findings
202207 bcchVersion of RecordPublishe
Subjective outcome evaluation of the community-based P.A.T.H.S Project : views of program implementers
202207 bcchVersion of RecordOthersThe Hong Kong Jockey Club Charities TrustPublishe
Evaluation of the project pATHS in mainland China : Findings based on student diaries
202012 bcrcVersion of RecordPublishe
Perceptions of adolescents, teachers and parents of life skills education and life skills in high school students in Hong Kong
202008 bcrcVersion of RecordEarly releas