12 research outputs found
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Time-Resolved Investigations of Electronic Transport Dynamics in Quantum Cascade Lasers Based on Diagonal Lasing Transition
In this study, the nature of electronic transport in
quantum cascade lasers (QCLs) has been extensively investigated
using an ultrafast time-resolved, degenerate, pump-probe optical
technique. Our investigations enable a comprehensive understanding
of the gain recovery dynamics in terms of a coupling
of the electronic transport to the oscillating intracavity laser
intensity. In QCLs that have a lasing transition diagonal in real
space, studies of the near-threshold reveal that the transport of
electrons changes bias region from phonon-limited relaxation
(tens of picoseconds) below threshold to photon-driven transport
via stimulated emission (a few picoseconds) above threshold. The
gain recovery dynamics in the photon-driven regime is compared
with conventional four-level lasers such as atomic, molecular, and
semiconductor interband lasers. The depopulation dynamics out
of the lower lasing state is explained using a tight-binding tunneling
model and phonon-limited relaxation. For the superlattice
relaxation, it is possible to explain the characteristic picosecond
transport via dielectric relaxation; Monte Carlo simulations with
a simple resistor model are developed, and the Esaki–Tsu model
is applied. Subpicosecond dynamics due to carrier heating in
the upper subband are isolated and appear to be at most about
10% of the gain compression compared with the contribution of
stimulated emission. Finally, the polarization anisotropy in the
active waveguide is experimentally shown to be negligible on our
pump-probe data, supporting our interpretation of data in terms
of gain recovery and transport.Engineering and Applied Science
Ultrafast dynamics and interlayer thermal coupling of hot carriers in epitaxial graphene
We report the first application of nondegenerate ultrafast pump-probe spectroscopy to investigate the dynamics of hot Dirac Fermions in epitaxial graphene. The DT spectra can be understood in terms of the effect of hot thermal carrier distributions on interband transitions with no electron-hole interaction. We also investigate the thermal coupling between carriers of doped and undoped layers. The coupling time is found to be below 500fs. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/61906/1/470_ftp.pd
Ultrafast electronic dynamics in unipolar n-doped indium gallium arsenide/gallium arsenide self-assembled quantum dots.
Photodetectors based on intraband infrared absorption in the quantum dots have demonstrated improved performance over its quantum well counterpart by lower dark current, relative temperature insensitivity, and its ability for normal incidence operation. Various scattering processes, including phonon emission/absorption and carrier-carrier scattering, are critical in understanding device operation on the fundamental level. In previous studies, our group has investigated carrier dynamics in both low- and high-density regime. Ultrafast electron-hole scattering and the predicted phonon bottleneck effect in intrinsic quantum dots have been observed. Further examination on electron dynamics in unipolar structures is presented in this thesis. We used n-doped quantum dot in mid-infrared photodetector device structure to study the electron dynamics in unipolar structure. Differential transmission spectroscopy with mid-infrared intraband pump and optical interband probe was implemented to measure the electron dynamics directly without creating extra electron-hole pair, Electron relaxation after excitation was measured under various density and temperature conditions. Rapid capture into quantum dot within ∼ 10 ps was observed due to Auger-type electron-electron scattering. Intradot relaxation from the quantum dot excited state to the ground state was also observed on the time scale of 100 ps. With highly doped electron density in the structure, the inter-sublevel relaxation is dominated by Auger-type electron-electron scattering and the phonon bottleneck effect is circumvented. Nanosecond-scale recovery in larger-sized quantum dots was observed, not intrinsic to electron dynamics but due to band-bending and built-in voltage drift. An ensemble Monte Carlo simulation was also established to model the dynamics in quantum dots and in goad agreement with the experimental results. We presented a comprehensive picture of electron dynamics in the unipolar quantum dot structure. Although the phonon bottleneck is circumvented with high doped electron density, relaxation processes in unipolar quantum dots have been measured with time scales longer than that of bipolar systems. The results explain the operation principles of the quantum dot infrared photodetector on a microscopic level and provide basic understanding for future applications and designs.Ph.D.Applied SciencesCondensed matter physicsElectrical engineeringOpticsPure SciencesUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/126328/2/3238118.pd
dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations.
To maintain a particular cell fate, a unique set of genes should be expressed while another set is repressed. One way to repress gene expression is through Polycomb group (PcG) proteins that compact chromatin into a silent configuration. In addition to cell fate maintenance, PcG proteins also maintain normal cell physiology, for example cell cycle. In the absence of PcG, ectopic activation of the PcG-repressed genes leads to developmental defects and malignant tumors. Little is known about the molecular nature of ectopic gene expression; especially what differentiates expression of a given gene in the orthotopic tissue (orthotopic expression) and the ectopic expression of the same gene due to PcG mutations. Here we present that ectopic gene expression in PcG mutant cells specifically requires dBRWD3, a negative regulator of HIRA/Yemanuclein (YEM)-mediated histone variant H3.3 deposition. dBRWD3 mutations suppress both the ectopic gene expression and aberrant tissue overgrowth in PcG mutants through a YEM-dependent mechanism. Our findings identified dBRWD3 as a critical regulator that is uniquely required for ectopic gene expression and aberrant tissue overgrowth caused by PcG mutations
The genetic interaction between <i>Scm</i> and <i>dBRWD3</i>.
<p>The genetic interaction between <i>Scm</i> and <i>dBRWD3</i>.</p
Ectopic expression of <i>Antp</i> is more sensitive to partial knockdown of <i>Taf5</i>, <i>Taf7</i>, <i>Cdk7</i>, and <i>CycH</i>.
<p>(A) Suppression of <i>Pc</i> depletion-induced ectopic expression of <i>Ubx</i> by depletion of <i>Taf5</i>, <i>Taf7</i>, <i>Cdk7</i>, and <i>CycH</i>. Quantification of Ubx positive neuron number in the doubly depleted brains as indicated. (B) The orthotopic expression of <i>Ubx</i> in the doubly depleted ventral nerve cords as indicated. (C) The orthotopic expression of <i>Ubx</i> in control, <i>Taf5</i>, <i>Taf7</i>, <i>Cdk7</i>, and <i>CycH</i> depleted ventral nerve cords. *** indicates p<0.0001 by Student's t-test. ns. indicates not significant. (D) The Trx-induced Abd-B expression (arrows) in the <i>CycH</i> knockdown wings. (E and F) The orthotopic expression of <i>Antp</i> in the <i>CycH</i> knockdown (E), and the control (F) wings. (G and H) The Abd-B expression in wings over-expressing both <i>trx</i> and <i>CycH</i> (G) or <i>CycH</i> alone (H) under the control of <i>ms1096-GAL4</i>. (I) The orthotopic expression of <i>Antp</i> in the <i>CycH</i> over-expression wings. Scale bars indicate 20μm.</p
The genetic interaction between <i>Psc</i> and <i>dBRWD3</i>.
<p>The genetic interaction between <i>Psc</i> and <i>dBRWD3</i>.</p
dBRWD3 suppresses ectopic expression of <i>upd</i> and activation of the JAK-STAT pathway.
<p>(A-C) The <i>upd1</i> (A), <i>upd2</i> (B), and <i>upd3</i> (C) mRNA levels of mosaic eye brain complexes isolated from <i>wild type</i>, <i>Scm</i><sup><i>D1</i></sup>, and <i>Scm</i><sup><i>D1</i></sup>, <i>dBRWD3</i><sup><i>s5349</i></sup> as indicated. Data are shown as means ± S.D. *, **, *** indicate P<0.01, 0.001, 0.0001 respectively by Student's t-test, n = 4. (D) A schematic illustration of a 3<sup>rd</sup> instar eye imaginal disc. MF stands for morphogenic furrow. The red square indicates the region examined in the following experiments. (E-I) Upd3 levels (arrows) in <i>Scm</i><sup><i>D1</i></sup> mutant clones (E), <i>Scm</i><sup><i>D1</i></sup>, <i>dBRWD3</i><sup><i>s5349</i></sup> double-mutant clones (F), <i>wild-type</i> clones (G), <i>Sce</i><sup><i>1</i></sup> mutant clones (H), and <i>dBRWD3</i><sup><i>s5349</i></sup>, <i>Sce</i><sup><i>1</i></sup> double-mutant clones (I) generated in 3<sup>rd</sup> instar eye imaginal discs by <i>ey-flp</i> and marked by the absence of GFP. Scale bars indicate 50μm. (J) Orthotopic Upd3 levels (arrows) in <i>dBRWD3</i><sup><i>s5349</i></sup> mutant clones generated in 2<sup>nd</sup> instar larval imaginal eye discs marked by the absence of GFP. Scale bars indicate 50μm. (K) Quantification analyses of orthotopic Upd3 levels (arrows) in <i>dBRWD3</i><sup><i>s5349</i></sup> mutant clones. ns. indicates not significant. (L) A schematic illustration of a 3<sup>rd</sup> instar antennal imaginal disc. The red square indicates the region examined in the following experiments. (M) GFP levels (arrows) of the 10XSTAT-nls-GFP reporter in a <i>wild-type</i> disc. (N-P) GFP levels (arrows) of the 10XSTAT-nls-GFP reporter in <i>Scm</i><sup><i>D1</i></sup> mutant clones (N), <i>Scm</i><sup><i>D1</i></sup>, <i>dBRWD3</i><sup><i>s5349</i></sup> double-mutant clones (O), and <i>dBRWD3</i><sup><i>s5349</i></sup> mutant clones (P) generated in 3<sup>rd</sup> instar antennal imaginal discs by <i>ey-flp</i> and marked by the absence of the <i>ubi</i> promoter driven mof-RFP. Scale bars indicate 50μm.</p
<i>dBRWD3</i><sup><i>s5349</i></sup> mutation suppresses ectopic expression of <i>Antp</i> in <i>Scm</i> <sup><i>D1</i></sup> or <i>Sce</i><sup><i>1</i></sup> mutant eye clones.
<p>(A) A schematic illustration of a 3<sup>rd</sup> instar eye imaginal disc. The red square indicates the region examined in the following experiments. (B-F) Antennapedia (Antp) levels (arrows) in <i>wild-type</i> clones (B), <i>Scm</i><sup><i>D1</i></sup> mutant clones (C), <i>Scm</i><sup><i>D1</i></sup>, <i>dBRWD3</i><sup><i>s5349</i></sup> double-mutant clones (D), <i>Sce</i><sup><i>1</i></sup> mutant clones (E), and <i>dBRWD3</i><sup><i>s5349</i></sup> <i>Sce</i><sup><i>1</i></sup> double-mutant clones (F) generated in the 3<sup>rd</sup> instar eye imaginal discs by <i>ey-flp</i> and marked by the absence of GFP. Scale bars indicate 50μm. (G) A schematic illustration of a 3<sup>rd</sup> instar wing imaginal disc. V and D stand for ventral and dorsal (marked by grey) compartments, respectively. (H) Antp levels (arrows) in a <i>wild-type</i> wing. (I) Antp levels (arrowheads) in <i>dBRWD3</i><sup><i>s5349</i></sup> mutant wing disc clones generated by <i>hs-flp</i> and marked by the absence of GFP. Scale bars indicate 50μm.</p