Genome-wide DNA methylation and transcriptomic profiles in the lifestyle strategies and asexual development of the forest fungal pathogen Heterobasidion parviporum

Heterobasidion parviporum is the most devastating fungal pathogen of conifer forests in Northern Europe. The fungus has dual life strategies, necrotrophy on living trees and saprotrophy on dead woods. DNA cytosine methylation is an important epigenetic modification in eukaryotic organisms. Our presumption is that the lifestyle transition and asexual development in H. parviporum could be driven by epigenetic effects. Involvements of DNA methylation in the regulation of aforementioned processes have never been studied thus far. RNA-seq identified lists of highly induced genes enriched in carbohydrate-active enzymes during necrotrophic interaction with host trees and saprotrophic sawdust growth. It also highlighted signaling- and transcription factor-related genes potentially associated with the transition of saprotrophic to necrotrophic lifestyle and groups of primary cellular activities throughout asexual development. Whole-genome bisulfite sequencing revealed that DNA methylation displayed pronounced preference in CpG dinucleotide context across the genome and mostly targeted transposable element (TE)-rich regions. TE methylation level demonstrated a strong negative correlation with TE expression, reinforcing the protective function of DNA methylation in fungal genome stability. Small groups of genes putatively subject to methylation transcriptional regulation in response to saprotrophic and necrotrophic growth in comparison with free-living mycelia were also explored. Our study reported on the first methylome map of a forest pathogen. Analysis of transcriptome and methylome variations associated with asexual development and different lifestyle strategies provided further understanding of basic biological processes in H. parviporum. More importantly, our work raised additional potential roles of DNA methylation in fungi apart from controlling the proliferation of TEs.Peer reviewe

Comparison between emerging adults and adults in terms of contamination fear, post-COVID-19 PTSD and psychiatric comorbidity

The present study compared Chinese emerging adults and adults regarding the association between contamination fear, posttraumatic stress disorder post-COVID-19 and psychiatric comorbidity after controlling for demographic and trauma exposure variables. 1089 Chinese civilians (M = 382; F = 707) with a mean age of 26 years (M = 26.36, SD = 8.58) were recruited from different provinces in China via an online survey posted on mainstream Chinese social networking platforms. They completed a demographic page with questions on trauma exposure, the Vancouver Obsessional Compulsive Inventory, the Posttraumatic Stress Disorder Checklist for DSM-5 and the General Health Questionnaire-28. Results showed that 12.7%, 68.7% and 18.6% met criteria for full, partial and no PTSD, respectively. Emerging adults reported significantly lower levels of symptoms of re-experiencing, avoidance, somatic problems, anxiety and fear of contamination than adults. In both emerging adults and adults, contamination fear was correlated with PTSD and psychiatric comorbidity. High educational attainment was significantly correlated with psychiatric comorbidity in emerging adults, but with PTSD in adults. Length of quarantine was correlated with psychiatric comorbidity only in adults. In conclusion, both emerging adults and adults developed varying levels of contamination fear, posttraumatic stress and general psychological symptoms following the outbreak of COVID-19. Emerging adults were more resilient than adults in coping with distress

Seismic Performance Assessment of Water Distribution Systems Based on Multi-Indexed Nodal Importance

open access articleSeismic performance assessment of water distribution systems (WDSs) based on hydraulic simulation is essential for resilience evaluation of WDSs under earthquake disasters. The assessment is mainly to determine how the water supply will be affected due to pipe breaks caused by the earthquake, with the water supply loss estimated based on the loss of supply to nodes. Existing research works usually use the average or overall performance metric of all user nodes as the system performance indicator without considering user nodes' individual performance and criticality. This paper proposes a framework to evaluate the importance of user nodes considering post-earthquake rescue service and the seismic performance of individual user nodes in the WDS, which supports the pipeline renovation plan to improve the performance of critical user nodes. The importance of user nodes is evaluated by a multi-index model, including the indices for daily service, post-earthquake rescue service, and network topology influence of user nodes. These indices evaluate the importance of user nodes in terms of their roles for daily water service, emergent rescue service, and water transmission to other nodes, respectively. Fragility model of pipelines evaluates the earthquake-induced damages of the WDS, and the seismic performance assessment of the WDS system is performed by the hydraulic model of the WDS with pipeline damages. The proposed framework is implemented in an actual WDS; the results show that the importance classification to user nodes by multi-index approach can identify the critical user nodes for post-earthquake rescue service, which traditional methods may ignore. The importance classification and seismic performance of individual user nodes make it feasible to check the seismic performance of critical user nodes and formulate a targeted pipeline renovation plan to focus limited resources on critical user nodes

Effect of Thermal Bridge in Light-Frame Wood Wall

The presence of thermal bridges in a wall increases local heat conduction of the building envelope, resulting in a decrease in the wall’s average thermal resistance. Simultaneously, the internal surface temperature of thermal bridge is lower than that of the surrounding areas and shows a tendency of condensation. Therefore, it is necessary to employ thermal bridges in the stage of construction design. In the research, a two-dimensional steady-state numerical simulation was performed targeting thermal bridges with light-frame wood wall. Meanwhile, the heat bridge effect was simulated under different circumstances by changing the types of insulation and cladding materials, the number of the studs, and the framing factor. The results showed that the linear heat transfer coefficient increased linearly as the studs and framing factor rose. After the test was validated, the relative error rate between the simulated correction coefficients and the experimentally derived correction coefficients was 11.4%, indicating that the correction coefficients can be simulated

Spatiotemporal distribution and particle-water partitioning of polycyclic aromatic hydrocarbons in Bohai Sea, China

The Bohai Sea is one of the most polluted marine areas in China with polycyclic aromatic hydrocarbons (PAHs) due to its unique hydrological and geographical environment. To investigate differences in PAH concentrations, composition, and particle-water partitioning before and after the rainy reason, water samples were collected during two surveying voyages covering most of the area of the Bohai Sea. Total and dissolved PAH concentrations were higher during the June voyage (total PAHs: 32.29 & PLUSMN; 15.18 ng/L, dissolved PAHs: 31.25 & PLUSMN; 15.26 ng/L) than the August voyage (total PAHs: 15.98 & PLUSMN; 6.39 ng/L, dissolved PAHs: 11.21 & PLUSMN; 5.59 ng/L). The opposite trend was observed for particulate PAHs (June: 1.04 & PLUSMN; 1.01 ng/L, August: 4.78 & PLUSMN; 2.96 ng/L). Among particulate PAHs, an unusually high proportion (65.07%) of low-molecular-weight (LMW) PAHs was observed during the August voyage, which was significantly higher than the proportion during the June voyage (21.86%). This high proportion was inconsistent with the general distribution of PAHs in the aquatic environment according to their physicochemical properties. The excess LMW PAHs adsorbed on suspended particulate matter arose mainly from soil affected by the petrochemical industry of the Bohai Economic Rim, and were carried with particles on runoff into the Bohai Sea during the rainy season. An estimated 5.49 t of LMW PAHs transitioned from the particulate phase to the dissolved phase during the rainy season. This transfer of LMW PAHs from coastal soil to the water column may be an important source of PAHs in the Bohai Sea

Deposition flux and mass inventory of polychlorinated biphenyls in sediments of the Yangtze River Estuary and inner shelf, East China Sea: Implications for contributions of large-river input and e-waste dismantling

Surface sediment samples were collected from the Yangtze River Estuary (YRE) to the inner-shelf mud area of the East China Sea (ECS) for a comprehensive study of the sources and fates of polychlorinated biphenyls (PCBs) based on their spatial distribution, deposition flux, and mass inventory. The total concentrations of 32 PCBs (Sigma(32)PCBs) varied from 0.3 to 11.9 ng/g dry weight. Under strong hydrodynamic conditions, the weak correlations between TOC or MD and Sigma 32PCB concentrations were observed in the YRE. In contrast, there were relatively well relationships of PCBs with TOC content and sediment grain size in the inner shelf of the ECS due to the influence of hydrological sorting from the YRE to the inner shelf of the ECS. This suggests that the Yangtze River input plays a considerable role in controlling the distribution of PCBs in the coastal ECS. Compared with the annual discharge of Sigma(32)PCBs from the Yangtze River to the sea (3.21 t/yr), the deposition flux was estimated to be similar to 2.63 t/yr. Furthermore, a total mass inventory of 50 tons in the sediments suggests that the YRE and inner shelf mud of the ECS represents an important global sink of PCBs. Estimated 21-39% of sedimentary PCBs were derived from local emissions (mainly dismantling of electronic waste) aside from Yangtze River input. Higher proportions of penta-CBs were also observed near the central Zhejiang Coast, providing further evidence that the local emission from e-waste dismantling near the coast is a significant contributor to sedimentary PCBs in the coastal ECS. (C) 2018 Elsevier B.V. All rights reserved

Atmospheric occurrence, transport and gas-particle partitioning of polychlorinated biphenyls over the northwestern Pacific Ocean

Ship-board air samples were collected during March to May 2015 from the East China Sea (ECS) to the northwestern Pacific Ocean (NWP) to explore the atmospheric occurrence and gas particle partitioning of polychlorinated biphenyls (PCBs) when the westerly East Asian Monsoon prevailed. Total PCB concentrations in the atmosphere ranged from 56.8 to 261 pg m(-3). Higher PCB levels were observed off the coast and minor temperature-induced changes showed that continuous emissions from East Asia remain as an important source to the regional atmosphere. A significant relationship between K-oa (octanol air partition coefficient) and K-p (gas particle partition coefficient) for PCBs was observed under continental air masses, suggesting that land-derived Organic aerosols affected the PCB gas particle partitioning after long-range transport, while an absence of this correlation was identified in marine air masses. The PCB partitioning cannot be fully explained by the absorptive mechanism as the predicted Kp were found to be 2-3 orders of magnitude lower than the measured K-p, while the prediction was closely matched when soot adsorption was considered. The results suggested the importance of soot carbon as a transport medium for PCBs during their long-range transport and considerable impacts of continental outflows on PCBs across the downwind area. The estimated transport mass of particulate PCBs into the ECS and NWP totals 2333 kg during the spring, constituting ca. 17% of annual emission inventories of unintentionally produced PCB in China. (C) 2017 Elsevier Ltd. All rights reserved

Deposition records of persistent organic pollutants and black carbon in dated sediment cores from China marginal seas: Implications for terrestrial sources and transport processes

Black carbon (BC) and persistent organic pollutants (POPs) were analyzed from three sediment cores collected offshore in the East China Marginal Seas. The results showed steadily increasing or stable BC concentrations and fluxes. By contrast, time trends of POPs fluxes were consistent with historical records of commercial production and use in China. Although the POP inventories decreased significantly with increase in offshore distance, the relatively consistent trends for individual POPs in different sea areas confirmed that the main sources are derived from mainland China and that atmospheric input was an important contribution. POPs inventories decreased by 59–91 % during transport from the Yellow Sea to the remote East China Sea and deposition to the sediment. This suggests that the source signal for POPs may be preserved under stable depositional environments, even though only a fraction of those pollutants are buried in open sea sediments

The FDA-approved anti-amyloid-β monoclonal antibodies for the treatment of Alzheimer’s disease: a systematic review and meta-analysis of randomized controlled trials

Abstract Background Alzheimer’s disease (AD) is a worldwide public health problem and is difficult to cure. Drugs aimed at slowing the progression of the disease have been developed, with the Food and Drug Administration (FDA) granting accelerated approval for aducanumab on June 21, 2021 and a new accelerated approval for lecanemab on January 22, 2023. We performed this systematic review and meta-analysis to assess the efficacy and safety of FDA-approved anti-amyloid-β (anti-Aβ) monoclonal antibodies (mabs) for the treatment of AD. Method PubMed, Embase, and Cochrane Library were systematically searched to identify relevant studies published before May 2023. Efficacy outcomes included Aβ, neuroimaging, and biomarker outcomes. Safety outcomes included amyloid-related imaging abnormalities with edema or effusions (ARIA-E) and ARIA with cerebral microhemorrhages, cerebral macrohemorrhages, or superficial siderosis (ARIA-H). Review Manager 5.4 software was used to assess the data. The standard mean differences (SMDs) or odds ratio (OR) with 95% confidence interval (95% CI) were analyzed and calculated with a random effect model or a fixed effect model. Result Overall, 4471 patients from 6 randomized controlled trials (RCTs), with 2190 patients in the treatment group and 2281 patients in the placebo group meeting the inclusion criteria. FDA-approved anti-Aβ mabs showed statistically significant improvements in clinical outcomes, including CDR-SB (P = 0.01), ADCS-ADL-MCI (P = 0.00003), ADCOMS (P < 0.00001), ADAS-Cog (P < 0.00001). Moreover, FDA-approved anti-Aβ mabs increased cerebrospinal fluid (CSF) Aβ1-42 (P = 0.002) and plasma Aβ42/40 ratios (P = 0.0008). They also decreased CSF P-Tau (P < 0.00001), CSF T-Tau (P < 0.00001), and plasma p-tau181 (P < 0.00001). FDA-approved anti-Aβ mabs perform neuroimaging changes in amyloid Positron Emission Tomography Standardized Uptake Value ratio (PET SUVr) (P < 0.00001). However, compared with placebo, FDA-approved anti-Aβ mabs had higher risk of ARIA-E (P < 0.00001) and ARIA-H (P < 0001). Conclusion FDA-approved anti-Aβ mabs have a role in slowing disease progression in patients with AD, at the cost of an increased probability of side effects