Determining the Phosphorus Release Curve for Sunphase HT Phytase from 250 to 2,000 FTU/kg in Nursery Pig Diets

A total of 280 pigs (DNA 241 × 600; initially 22.9 ± 0.52 lb BW) were used in a 21-d growth study to determine the available P (aP) release curve for Sunphase HT phytase (Wuhan Sunhy Biology Co., Ltd.; Wuhan, P.R. China). At approximately 19 d of age, pigs were weaned, randomly allotted to pens, and fed common starter diets. Pigs were blocked by average pen body weight (BW) and randomly allotted to 1 of 7 dietary treatments on d 21 post-weaning, considered d 0 of the study. Dietary treatments were derived from a single basal diet, and ingredients including phytase, monocalcium P, limestone, and sand were added to create the treatment diets. Treatments included 3 diets containing increasing (0.11, 0.19, and 0.27% aP) inorganic P from monocalcium P, or 4 diets with increasing phytase (250, 500, 1,000, or 2,000 FTU/kg) added to the diet containing 0.11% aP. All diets were corn-soybean meal-canola meal-based and were formulated to contain 1.24% SID Lys and an analyzed Ca:P ratio of 1.10:1. Prior to the beginning of the study, all pigs were fed a diet containing 0.11% aP for a 3-d period (d 18 to 21 post-weaning). At the conclusion of the study, 1 pig, closest to the mean weight of each pen, was euthanized and the right fibula, rib, and metacarpal were collected to determine bone ash and density. For the overall experimental period, pigs fed increasing levels of aP from inorganic P had improved (linear, P ≤ 0.014) ADG, F/G, and final BW. Similarly, pigs fed increasing phytase had increased (linear, P ≤ 0.011) ADG and final BW as well as improved (quadratic, P = 0.017) F/G. For fibula bone ash weight and percentage bone ash, rib bone ash weight and bone density, and all metacarpal bone properties, pigs fed increasing levels of aP from inorganic P exhibited a linear improvement (P ≤ 0.019), with a quadratic response (P ≤ 0.030) for fibula bone density and rib percentage bone ash. Additionally, pigs fed increasing phytase had increased (P \u3c 0.05) bone ash weight, percentage bone ash, and bone density in either a linear or quadratic fashion depending on the bone analyzed. The available P release curve generated for Sunphase HT for percentage bone ash combining values from right fibula, rib, and metacarpal is: aP = (0.360 × FTU) ÷ (2,330.250 + FTU)

Identification of the Characteristic Scale of Fine Ground Objects: A Case Study of the Core Observation Area in the Middle Reaches of the Heihe River Basin

Capturing the spatial heterogeneity and characteristic scale is the key to determining the spatial patterns of land surfaces. In this research, the core observation area of the middle reaches of the Heihe River Basin was selected as the study area, and the scale identification of several typical objects was carried out by implementing experiments on moderate- and high-resolution remotely sensed ASTER and CASI NDVI images. The aim was to evaluate the potential of the local variance and semivariance analysis to characterize the spatial heterogeneity of objects, track their changes with scale, and obtain their scales. Our results show that natural objects have multiscale structures. For a single object with a recognizable size, the results of the two methods are relatively consistent. For continuously distributed samples of indistinctive size, the scale obtained by the local variance is smaller than that obtained by the semivariance. As the image resolution becomes coarser and the research scopes expand, the scales of objects are also increasing. This article also indicates that with a small research area of uniform objects, the local variance and semivariance are easy to facilitate researchers to quickly select the appropriate spatial resolution of remote sensing data according to the research area

Discovering the Mechanisms of Oleodaphnone as a Potential HIV Latency-Reversing Agent by Transcriptome Profiling

Latent HIV is a key factor that makes AIDS difficult to cure. Highly effective and specific latent HIV activators can effectively activate latent HIV, and then combined with antiretroviral therapy to achieve a functional cure of AIDS. Here, four sesquiterpenes (1–4) including a new one (1), five flavonoids (5–9) including three biflavonoid structures, and two lignans (10 and 11) were obtained from the roots of Wikstroemia chamaedaphne. Their structures were elucidated through comprehensive spectroscopic analyses. The absolute configuration of 1 was determined by experimental electronic circular dichroism. NH2 cell model was used to test the activity of these 11 compounds in activating latent HIV. Oleodaphnone (2) showed the latent HIV activation effect as well as the positive drug prostratin, and the activation effect was time- and concentration-dependent. Based on transcriptome analysis, the underlying mechanism was that oleodaphnone regulated the TNF, C-type lectin receptor, NF-κB, IL-17, MAPK, NOD-like receptor, JAK-Stat, FoxO, and Toll-like receptor signaling pathways. This study provides the basis for the potential development of oleodaphnone as an effective HIV latency-reversing agent

Identification and Validation of Autophagy-Related Genes in Vitiligo

Vitiligo is a common depigmented disease with unclear pathogenesis. Autophagy is crucial for maintaining cellular homeostasis and has been linked to a variety of autoimmune disorders; however, there have been no reports exploring the involvement of autophagy-related genes (ARGs) in vitiligo using bioinformatics methodologies. In this study, RNA-sequencing technology was used to identify the differentially expressed genes (DEGs) and the Human Autophagy Database (HADb) was overlapped to identify differentially expressed autophagy-related genes (DEARGs) in stable non-segmental vitiligo (NSV). Bioinformatics analyses were conducted with R packages and Ingenuity Pathways Analysis (IPA). DEARGs were further confirmed with qRT-PCR. Critical autophagy markers were detected with Western blotting analysis. We identified a total of 39 DEARGs in vitiligo lesions. DEARGs-enriched canonical pathways, diseases and bio functions, upstream regulators, and networks were discovered. qRT-PCR confirmed the significant increases in FOS and RGS19 in vitiligo lesions. Lower microtubule-associated protein 1 light chain (LC3) II/LC3I ratio and higher sequestosome 1 (SQSTM1, p62) expression were found in vitiligo lesions. In conclusion, this study provided a new insight that autophagy dysregulation appeared in stable vitiligo lesions and might be involved in the etiology of vitiligo by taking part in multiple pathways and bio functions

Identification and Validation of Autophagy-Related Genes in Vitiligo

Vitiligo is a common depigmented disease with unclear pathogenesis. Autophagy is crucial for maintaining cellular homeostasis and has been linked to a variety of autoimmune disorders; however, there have been no reports exploring the involvement of autophagy-related genes (ARGs) in vitiligo using bioinformatics methodologies. In this study, RNA-sequencing technology was used to identify the differentially expressed genes (DEGs) and the Human Autophagy Database (HADb) was overlapped to identify differentially expressed autophagy-related genes (DEARGs) in stable non-segmental vitiligo (NSV). Bioinformatics analyses were conducted with R packages and Ingenuity Pathways Analysis (IPA). DEARGs were further confirmed with qRT-PCR. Critical autophagy markers were detected with Western blotting analysis. We identified a total of 39 DEARGs in vitiligo lesions. DEARGs-enriched canonical pathways, diseases and bio functions, upstream regulators, and networks were discovered. qRT-PCR confirmed the significant increases in FOS and RGS19 in vitiligo lesions. Lower microtubule-associated protein 1 light chain (LC3) II/LC3I ratio and higher sequestosome 1 (SQSTM1, p62) expression were found in vitiligo lesions. In conclusion, this study provided a new insight that autophagy dysregulation appeared in stable vitiligo lesions and might be involved in the etiology of vitiligo by taking part in multiple pathways and bio functions

Interpretation of and lessons from the guidelines on infection prevention and control of old-age institutions in the context of COVID-19 pandemic

This paper interprets the content and recommendations of the guidelines on infection prevention and control in long-term care facilities put forward by the World Health Organization （WHO） during the 2019 coronavirus disease （COVID-19） pandemic， and actively explores the key points of nursing and infection prevention and control measures for the long-term care facilities under the background of repeated outbreaks， with the aim of providing care measures and infection prevention and control measures that suit our national conditions to improve the living standards of the elderly and protect them from viral infection amid the recurring pandemic