Swimming Exercise-Induced Improvements in Cardiorespiratory Fitness (CRF) are Caused by Nitric Oxide Functional Adaptations in the Oxygen Transport System

Cardiorespiratory fitness (CRF) is associated with referring to enhance oxygen transport capacity to respiratory systems and increasing oxygen transport circulatory to skeletal muscle to produce energy. The aim of this report on the health-related CRF in the oxygen transport system-mediated physiological nitric oxide (NO) functional adaptations. Therefore, we want to know that swimming exercise-induced improvements in CRF resulted in increased oxygen transport capacity during physical activity of the respiratory systems. Therefore, the oxygen circulatory transport system is related to NO signaling and has been associated with various pathophysiologic functions and neuronal activity. Besides mediating normal functions, NO is implicated in inflammation and hypertension disease states. Swimming exercise is a good way to increase the rate of metabolism. Swimming exercise improves heart rate and oxygen circulatory, and increases the rate of metabolism and burning of heat. In this context, this review summarizes the roles of NO in improvements in cardiorespiratory fitness

Combined Ketogenic Diet and Walking Exercise Interventions in Community Older Frailty and Skeletal Muscle Sarcopenia

The ketogenic diet and walking exercise training interventions are two key public health lifestyle factors. The potential of combined lifestyle factors interventions focused on getting to compliance in diet and exercise. A balanced ketogenic diet and regular exercise interventions is key modifiable factor to the prevention and management of community older frailty and skeletal muscle sarcopenia. Influence health across the lifespan and reduction of the risk of premature death through several biochemistry mechanisms. Community older group’s lifestyle factors interventions contribute identity in their natural living environment. While the older health benefits of walking exercise training interventions strategies are commonly to study, combining ketogenic diet and walking exercise interventions can induce greater benefits in community older groups

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis

We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flanking primers and one mutagenic primer with different melting temperatures (T(m)) are used together in a single PCR tube continuously without any intervention. A single-stranded mutagenic DNA (smDNA) is synthesized utilizing the high T(m) mutagenic primer at a high annealing temperature, which prevents the priming of the low T(m) primers (i.e. the two flanking primers). A megaprimer is then produced using this smDNA as the template at a denaturing temperature that prevents wild-type template DNA activity. The desired mutant DNA is then obtained by cycling again through these first two steps, resulting in a mutagenic efficiency of 100% in all tested cases. This highly automated method not only eliminates the necessity of any intermediate manipulation and accomplishes the mutagenesis process in a single round of PCR but, most notably, enables complete success of mutagenesis. This novel method is also both cost and time efficient and fully automated

Enhanced Monochromatic Photon Emission from Millicharged Co-Interacting Dark Matter

We study a millicharged co-interacting dark matter scenario, where the primary dark matter constituent is the dark photon $A'$ and the secondary component is the fermion $\chi$. In this model, $\chi$ interacts with $A'$ via a $U(1)'$ interaction while being millicharged with respect to normal photons. Our investigation focuses on the oscillation of $A'$ dark matter into photons within the background of $\chi$ particles, revealing that the $A'-\chi$ scattering rate benefits from a Bose enhancement of the $A'$ final state. As the oscillation production rate is directly linked to the scattering rate, the conversion of $A'$ dark matter into monochromatic photons experiences significant amplification owing to this Bose enhancement, especially when the scattering rate $\Gamma_{\rm sca}$ approaches the dark photon mass $m_{A'}$. These converted monochromatic photons are detectable through radio telescopes and can induce distortions in the Cosmic Microwave Background (CMB) spectrum. We find that the sensitivity of radio telescopes and the constraints imposed by CMB distortion on the kinetic mixing parameter are notably heightened compared to scenarios without the subdominant millicharged dark matter.Comment: 9 pages, 1 figur

Amplifying Non-Resonant Production of Dark Sector Particles in Scattering Dominance Regime

We investigate the enhancement of dark sector particle production within the scattering dominant regime. These particles typically exhibit a slight mixing with Standard Model particles through various portals, allowing for their generation through in-medium oscillation from Standard Model particle sources. Our analysis reveals that in the scattering dominance regime, with a significantly smaller scattering mean free path $\lambda_{\rm sca}$ compared to the absorption mean free path $\lambda_{\rm abs}$, the non-resonant production of sterile states can experience an enhancement by a factor of $\lambda_{\rm abs}/\lambda_{\rm sca}$. This phenomenon is demonstrated within the context of kinetic mixing dark photon production at a reactor, precisely satisfying this condition. By incorporating this collisional enhancement, we find that the current sensitivity to the mixing parameter $\epsilon$ for dark photons in the TEXONO experiment can be significantly improved across a range spanning from tens of eV to MeV. This advancement establishes the most stringent laboratory constraint within this mass spectrum for the dark photon. Sterile neutrino production, however, does not exhibit such enhancement, either due to the failure to meet the scattering dominance criterion or the neutrino damping in resonant production.Comment: 8 pages, 4 figure

Ossification patterns of the carpus and tarsus in salamanders and impacts of preaxial dominance on the fin-to-limb transition

Early limb skeletogenesis in salamanders is characterized by preaxial elements, digits I and II forming earlier than their postaxial counterparts (digits III to V), a phenomenon known as preaxial dominance, whereas in amniotes and anurans, these developmental sequences are reversed. This pattern characterizes the late skeletogenesis of digits and zeugopodium of anamniote tetrapods but remains unknown in carpals/tarsals. To correct this gap in knowledge, we investigate the ossification patterns of the carpals/tarsals in six salamander families/clades based on micro–computed tomography scans. We found that preaxial dominance is seen in the distal carpals/tarsals of several salamander clades and diverse early tetrapods, such as temnospondyls and amniotes. This distribution suggests that preaxial dominance is a primitive developmental pattern in tetrapods. Our results demonstrate that the distal carpals/tarsals are developmentally and evolutionarily independent in the autopodium, and preaxial dominance facilitates stabilization of the number of distal carpals/tarsals during fin-to-limb transition and digit reduction in early tetrapods

Effects of matrine on collagen proliferation and TNF-α, TGF-β1 and CTGF in atrial tissues of dogs with persistent atrial fibrillation

目的 探讨苦参碱对犬心房颤动（房颤）心房肌组织中胶原合成以及肿瘤坏死因子（tumor necrosis factor alpha,TNF-α）、转化生长因子（transforming growth factor-β1，TGF-β1）和结缔组织生长因子（connective Tissue Growth Factor，CTGF）表达变化的影响。方法 健康比格犬10只采用快速右心室起搏造房颤模型，随机分成房颤组和房颤+苦参碱组各5只。采用天狼星红染色，计算胶原容积分数（collagen volume fraction，CVF）以测定纤维化程度；采用免疫组织化学法检测右心房TNF-α、TGF-β1和CTGF的蛋白表达情况；用逆转录-聚合酶链反应（RT-PCR）技术检测TNF-α、TGF-β1和CTGF的mRNA水平表达情况。结果 与房颤组相比，房颤+苦参碱组纤维化程度降低，CVF明显下降（P＜0.05），TNF-α、TGF-β1和CTGF蛋白表达水平下降，且TNF-α和TGF-β1的mRNA表达水平显著下降（P＜0.05，P＜0.01）。结论 苦参碱可能通过抑制TNF-α、TGF-β1和CTGF的表达，抑制房颤心房肌胶原合成，改善心房组织纤维化程度。Objective:To study the effects of matrine (mat) on collagen synthesis and expression of tumor necrosis factoralpha (TNF-α), and transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in atrial tissues of dogs with persistent atrial fibrillation (AF). Methods : Ten healthy beagle dogs were randomly divided into two groups: AF group (n=5) and AF/Mat group (n=5), using right ventricular pacing to establish AF model. The collagen volume fraction (CVF) in atrial tissue were detected by sirius red staining to determine the level of fabrication. The level of TNF-α, TGF-β1 and CTGF were detected by immunohisto-chemistry. The mRNA expression level of TNF-α, TGF-β1 and CTGF were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results:  Compared with the AF group, the fabriation level of AF/Mat was decreased obviously (P<0.05), the expression levels of TNF-α, TGF-β1 and CTGF were decreased, and the mRNA expression level were decreased significantly in atrial tissues (P<0.05 and P<0.01). Conclusion: Matrine may inhibits fabrosis in atrial tissues through inhibition collagen proliferation and expression of TNF-α, TGF-β1 and CTGF