目的 探讨苦参碱对犬心房颤动（房颤）心房肌组织中胶原合成以及肿瘤坏死因子（tumor necrosis factor alpha,TNF-α）、转化生长因子（transforming growth factor-β1，TGF-β1）和结缔组织生长因子（connective Tissue Growth Factor，CTGF）表达变化的影响。方法 健康比格犬10只采用快速右心室起搏造房颤模型，随机分成房颤组和房颤+苦参碱组各5只。采用天狼星红染色，计算胶原容积分数（collagen volume fraction，CVF）以测定纤维化程度；采用免疫组织化学法检测右心房TNF-α、TGF-β1和CTGF的蛋白表达情况；用逆转录-聚合酶链反应（RT-PCR）技术检测TNF-α、TGF-β1和CTGF的mRNA水平表达情况。结果 与房颤组相比，房颤+苦参碱组纤维化程度降低，CVF明显下降（P＜0.05），TNF-α、TGF-β1和CTGF蛋白表达水平下降，且TNF-α和TGF-β1的mRNA表达水平显著下降（P＜0.05，P＜0.01）。结论 苦参碱可能通过抑制TNF-α、TGF-β1和CTGF的表达，抑制房颤心房肌胶原合成，改善心房组织纤维化程度。Objective:To study the effects of matrine (mat) on collagen synthesis and expression of tumor necrosis factoralpha (TNF-α), and transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in atrial tissues of dogs with persistent atrial fibrillation (AF). Methods : Ten healthy beagle dogs were randomly divided into two groups: AF group (n=5) and AF/Mat group (n=5), using right ventricular pacing to establish AF model. The collagen volume fraction (CVF) in atrial tissue were detected by sirius red staining to determine the level of fabrication. The level of TNF-α, TGF-β1 and CTGF were detected by immunohisto-chemistry. The mRNA expression level of TNF-α, TGF-β1 and CTGF were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: Compared with the AF group, the fabriation level of AF/Mat was decreased obviously (P<0.05), the expression levels of TNF-α, TGF-β1 and CTGF were decreased, and the mRNA expression level were decreased significantly in atrial tissues (P<0.05 and P<0.01). Conclusion: Matrine may inhibits fabrosis in atrial tissues through inhibition collagen proliferation and expression of TNF-α, TGF-β1 and CTGF
