JNK Signalling Regulates Self-Renewal of Proliferative Urine-Derived Renal Progenitor Cells via Inhibition of Ferroptosis

With a global increase in chronic kidney disease patients, alternatives to dialysis and organ transplantation are needed. Stem cell-based therapies could be one possibility to treat chronic kidney disease. Here, we used multipotent urine-derived renal progenitor cells (UdRPCs) to study nephrogenesis. UdRPCs treated with the JNK inhibitor—AEG3482 displayed decreased proliferation and downregulated transcription of cell cycle-associated genes as well as the kidney progenitor markers—SIX2, SALL1 and VCAM1. In addition, levels of activated SMAD2/3, which is associated with the maintenance of self-renewal in UdRPCs, were decreased. JNK inhibition resulted in less efficient oxidative phosphorylation and more lipid peroxidation via ferroptosis, an iron-dependent non-apoptotic cell death pathway linked to various forms of kidney disease. Our study is the first to describe the importance of JNK signalling as a link between maintenance of self-renewal and protection against ferroptosis in SIX2-positive renal progenitor cells

Espacialização de doenças da soja (Glycine max (L.) Merrill) nas safras 2014/2015, 2015/2016 e 2016/2017 no Estado de Mato Grosso.

O Estado do Mato Grosso, sendo o maior produtor de soja brasileiro, registra ocorrências de diversas doenças nesta cultura, com um custo total de manejo por ha de R$ 242,42 na safra de 2016/2017 (IMEA, 2017). Para se disponha de um prognóstico cada vez mais preciso das safras, desde a safra 2008/209, Aprosoja (Associação dos Produtores de Soja e Milho do Estado do Mato Grosso) coordena uma expedição técnica, composta por equipes que visitam os produtores rurais, identificando problemas e oportunidades da safra que se inicia, denominado de ?Raio-X da Safra?. Dentre as principais doenças registradas no Estado do Mato Grosso estão: antracnose, ferrugem-asiática, mancha-alvo e mofo-branco, dentre outras. Em função do impacto destas doenças na produtividade da soja, é importante utilizar ferramentas computacionais, tais como sistemas de informação geográfica (GIS) que auxiliem no mapeamento e monitoramento de eventos de doenças visando minimizar ou mitigar os efeitos das doenças e garantir a segurança agrícola. Este trabalho teve como objetivo mapear e caracterizar ocorrências de antracnose, ferrugem-asiática, mancha-alvo e mofo-branco nas safras 2014/2015, 2015/2016 e 2017/2017, no Estado de Mato Grosso, utilizando sistema de informação geográfica, adicionalmente verificar se houve aumento quantitativo das ocorrências e se estas ocorrem de maneira agrupada.Na publicação: MIGUEL-WRUCK, D.S

Espacialização de ferrugem asiática da soja (Phakopsora pachyrhizi) nas safras 2014-2015, 2015-2016 e 2016-2017 no estado de Mato Grosso.

Este trabalho teve como objetivo mapear e caracterizar ocorrências de ferrugem asiática, nas safras de 2014/2015, 2015/2016 e 2017/2017, no Estado de Mato Grosso, utilizando sistema de informação geográfica e adicionalmente verificar se houve aumento quantitativo das ocorrências e se estas ocorrem de maneira agrupada.Na publicação: MIGUEL-WRUCK, D. S

Biomass and leaf area in Eucalyptus clones in crop-livestock-forestry systems: implications for pruning.

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Human Amniocytes Are Receptive to Chemically Induced Reprogramming to Pluripotency

Restoring pluripotency using chemical compounds alone would be a major step forward in developing clinical-grade pluripotent stem cells, but this has not yet been reported in human cells. We previously demonstrated that VPA_ AFS cells, human amniocytes cultivated with valproic acid (VPA) acquired functional pluripotency while remaining distinct from human embryonic stem cells (hESCs), questioning the relationship between the modulation of cell fate and molecular regulation of the pluripotency network. Here, we used single-cell analysis and functional assays to reveal that VPA treatment resulted in a homogeneous population of self-renewing non-transformed cells that fulfill the hallmarks of pluripotency, i.e., a short G1 phase, a dependence on glycolytic metabolism, expression of epigenetic modifications on histones 3 and 4, and reactivation of endogenous OCT4 and downstream targets at a lower level than that observed in hESCs. Mechanistic insights into the process of VPA-induced reprogramming revealed that it was dependent on OCT4 promoter activation, which was achieved independently of the PI3K (phosphatidylinositol 3-kinase)/ AKT/ mTOR (mammalian target of rapamycin) pathway or GSK3 beta inhibition but was concomitant with the presence of acetylated histones H3K9 and H3K56, which promote pluripotency. Our data identify, for the first time, the pluripotent transcriptional and molecular signature and metabolic status of human chemically induced pluripotent stem cells

Spatial and temporal variations of soil water in an integrated crop-livestock-forestry system.

This study aimed to monitor the spatial and temporal variations of water tension in the soil in an iCLF in the Cerrado

Obstructive Sleep Apnea and 15-Year Cognitive Decline: The Atherosclerosis Risk in Communities (ARIC) Study

Prospective data evaluating abnormal sleep quality and quantity with cognitive decline are limited because most studies used subjective data and/or had short follow-up. We hypothesized that, over 15 y of follow-up, participants with objectively measured obstructive sleep apnea (OSA) and other indices of poor sleep quantity and quality would experience greater decline in cognitive functioning than participants with normal sleep patterns

Integrated crop-livestock-forestry systems as potential carbon sinks.

Mixed farming systems have great potential to combine food production with environmental services, including climate change mitigation and biodiversity preservation. This study contributed to the evaluation of integrated crop-livestock (ICL) and crop-livestock-forestry (ICLF) production systems from the point of view of their potential for carbon (C) sequestration in the soil and in the production system as a whole

Práticas e manejo de sistemas de integração lavoura-pecuária na safra e safrinha para as Regiões Centro-Oeste e Sudeste.

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