Effect of temperature on fatty acid metabolism in skeletal muscle mitochondria of untrained and endurance-trained rats - Fig 2

<p><b>Determination of protein levels in skeletal muscle homogenates (A) and mitochondria (B) from control (</b>c<b>) and trained (t) rats. A</b>, Representative Western blots and analyses of the protein expression of PGC1α, mitochondrial marker (Mito marker), Nrf2, CD36, β-actin and GADPH. <b>B</b>, Representative Western blots and analyses of the protein expression of ACADS, CPT1A, CD36, COXII, (Mito marker). Expression levels were normalized for β actin or GADPH (<b>A</b>, <i>right panel</i>) and mito marker or COXII (<b>B</b>, <i>right panel</i>). The data (± SD, <i>n</i> = 6) is from six independent homogenate or mitochondrial preparations from six different animals from each group. ***<i>P</i> < 0.001, **<i>P</i> < 0.01, *<i>P</i> < 0.05 vs. value obtained for control rats.</p

Effect of temperature on fatty acid metabolism in skeletal muscle mitochondria of untrained and endurance-trained rats - Fig 1

<p><b>The influence of endurance training on mitochondrial oxidation of palmitoylcarnitine (A) and glycerol-3-phosphate (B) at 25</b>°<b>C, 35</b>°<b>C and 42</b>°<b>C.</b> Respiratory rates were measured in mitochondria isolated from control (c) and trained (<b>t</b>) rats in the presence of 150 μM ADP (state 3, phosphorylating respiration) with 0.5 mM palmitoyl-DL-carnitine (<b>A</b>) or 5 mM glycerol-3-phosphate (<b>B</b>). Mean values (± SD) for six mitochondria preparations from six different animals from each group (<i>n</i> = 6) are shown. ^###<i>P</i> < 0.001, ^##<i>P</i> < 0.01, ^#<i>P</i> < 0.05 vs value obtained at 35°C for a given group of animals, i.e., within control rats or trained rats. ***<i>P</i> < 0.001, **<i>P</i> < 0.01, *<i>P</i> < 0.05 vs. value obtained for control rats for a given assay temperature.</p

Respiratory rates and coupling parameters in control and trained rat skeletal muscle mitochondria at 25°C, 35°C and 42°C.

<p>Respiratory rates and coupling parameters in control and trained rat skeletal muscle mitochondria at 25°C, 35°C and 42°C.</p