26 research outputs found
Using Feathers for Molecular Sexing of Straw-headed Bulbul (Pycnonotus zeylanicus) Offsprings
Sex determination of straw-headed bulbul offspring was carried out from 27 offspring’s plucked feather samples in a captive breeding program. Using direct PCR, this study provided more evidences that feather samples are reliable as a source of DNA for non-invasive and effective molecular sexing. The study also revealed that the offspring sex ratio of straw-headed bulbul was slightly inclined towards males, but there was no significant difference from the value of 0.5.
Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18
The prevalence of
intermediately virulent Rhodococcus
equi isolates from pig submaxillary
lymph nodes from four slaughterhouses in
Nakhonpathom province, Thailand, was
investigated. The isolates were tested for the
presence of virulence plasmids and the 20-kDa
virulence-associated protein antigen (VapB) gene
by PCR. Of the 734 submaxillary lymph nodes
tested, 19 (2.6%) produced positive cultures
of R. equi. All 19 isolates
were positive for the VapB gene and contained
virulence plasmids that were identified as type
1 (six isolates), type 6 (two isolates), type 7
(one isolate), type 16 (two isolates), and a new
variant (eight isolates). Based on the
restriction digestion patterns of the plasmid
DNAs, we tentatively designated the variant as
type 18. Investigation of the prevalence and
plasmid profiles of VapB-positive R.
equi in pigs should be extended
throughout Thailand to evaluate potential
sources of zoonotic infections
Short Communication: A new primer set in CHD1 gene for bird sex identification
Determine sex is difficult for many bird species that are sexually monomorphic or only dimorphic in the adult stage. Many molecular markers have been developed for DNA sexing, which were mostly based on identifying Z and W chromosomes of avians. The sex determination of birds was mostly applied universal primer set such as P2/P8 or 2550F/2718R. However, those universal primers that were designed sometimes could not good result consistency in non-ratite birds or ratite birds. Therefore, we improved a specific primer design with deletion site in W chromosome to amplify the female-specific segments on Chromodomain-helicase DNA binding protein-1 (CHD1) gene from alignment sequences CHD1-Z and CHD1-W of Macrocephalon maleo S. Müller, 1846. This study aims to design a new pair of primer targeting a section of the CHD1 gene that can be amplified in non-ratite birds, the name of a new primer is In-Sex F/In-Sex R. A new primer set amplified DNA fragments in around 650 or 550 base pairs of CHD1-Z and also, 350 base pairs of CHD1-W. The result has successfully amplified the sex of multiple species on orders Galliformes, Passeriformes, Accipitriformes, and Strigiformes. This analysis can be helpful to the effort of in-situ or ex-situ management and conservation programs e.g the mating system in birds. And also, the analysis can be helpful for sexing data in the case of captive birds before releasing in natural habitat or reintroduction programs. © 2021, Society for Indonesian Biodiversity. All rights reserved
PCR-based Restriction Fragment Length Polymorphism for Subtyping of Salmonella from Chicken Isolates
ABSTRACT Genotypic diversity in two flagellin genes, fliC and fljB, encoding phase-1 and phase-2 flagellin of Salmonella enterica, offers a potential biomarker for Salmonella subtyping. Forty-seven Salmonella isolates of 20 different serovars derived from chicken samples in Thailand were studied using the fliC/ fljB PCR-based RFLP assay. With two restriction endonucleases, MboI and HhaI, the fliC showed 11 and 9 patterns, while the fljB showed 6 and 7 patterns respectively. Though the PCR-based RFLP test cannot replace serotyping, the assay is based on the flagellin genes encoding proteins on the bacterial surface that are related to serotyping scheme. Overall, the assay was reproducible and successfully applied to simply screen Salmonella serovars as an alternative subtyping test for rapid traceability of Salmonella contamination in chicken production
Annual ovarian activity monitored by the noninvasive measurement of fecal concentrations of progesterone and 17β-estradiol metabolites in rusa deer (<i>Rusa timorensis</i>)
Mycobacterium tuberculosis Infection of Domesticated Asian Elephants, Thailand
Four Asian elephants were confirmed to be infected with Mycobacterium tuberculosis by bacterial culture, other diagnostic procedures, and sequencing of 16S–23S rDNA internal transcribed spacer region, 16S rRNA, and gyrase B gene sequences. Genotyping showed that the infectious agents originated from 4 sources in Thailand. To identify infections, a combination of diagnostic assays is essential