12 research outputs found
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application
Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
Exosomes,
naturally secreted nanoparticles, have been introduced
as vehicles for horizontal transfer of genetic material. We induced
autologous exosomes containing a cocktail of reprogramming factors
(“reprosomes”) to convert fibroblasts into neural progenitor
cells (NPCs). The fibroblasts were treated with ultrasound and subsequently
cultured in neural stem cell medium for 1 day to induce the release
of reprosomes composed of reprogramming factors associated with chromatin
remodeling and neural lineage-specific factors. After being treated
with reprosomes, fibroblasts were converted into NPCs (rNPCs) with
great efficiency <i>via</i> activation of chromatin remodeling,
so quickly that only 5 days were required for the formation of 1500
spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal
and proliferative properties for several weeks and successfully differentiated
into neurons, astrocytes, and oligodendrocytes <i>in vitro</i> and <i>in vivo</i>. Reprosome-mediated cellular reprogramming
is simple, safe, and efficient to produce autologous stem cells for
clinical application