NIMBUS-5 sounder data processing system. Part 2: Results

The Nimbus-5 spacecraft carries infrared and microwave radiometers for sensing the temperature distribution of the atmosphere. Methods developed for obtaining temperature profiles from the combined set of infrared and microwave radiation measurements are described. Algorithms used to determine (a) vertical temperature and water vapor profiles, (b) cloud height, fractional coverage, and liquid water content, (c) surface temperature, and (d) total outgoing longwave radiation flux are described. Various meteorological results obtained from the application of the Nimbus-5 sounding data processing system during 1973 and 1974 are presented

Variability in the extreme helium star LSS 5121

We report a photometric and spectroscopic study of the hot extreme helium star LSS 5121. We found photometric variability, but no period was evident in its periodogram. This is consistent with the previous proposal, based on spectral line variations, that LSS 5121 is a non-radial pulsator similar to other hot extreme helium stars.Comment: 5 pages, 6 figure

The Hot R Coronae Borealis Star DY Centauri is a Binary

The remarkable hot R Coronae Borealis (RCB) star DY Cen is revealed to be the first and only binary system to be found among the RCB stars and their likely relatives, including the extreme helium stars and the hydrogen-deficient carbon stars. Radial velocity determinations from 1982 to 2010 have shown that DY Cen is a single-lined spectroscopic binary in an eccentric orbit with a period of 39.67 days. It is also one of the hottest and most H-rich member of the class of RCB stars. The system may have evolved from a common envelope to its current form.Spanish Ministry of Economy and Competitiveness AYA-2011-27754McDonald Observator

Targeting tumor multicellular aggregation through IGPR-1 inhibits colon cancer growth and improves chemotherapy

Adhesion to extracellular matrix (ECM) is crucially important for survival of normal epithelial cells as detachment from ECM triggers specific apoptosis known as anoikis. As tumor cells lose the requirement for anchorage to ECM, they rely on cell-cell adhesion 'multicellular aggregation' for survival. Multicellular aggregation of tumor cells also significantly determines the sensitivity of tumor cells to the cytotoxic effects of chemotherapeutics. In this report, we demonstrate that expression of immunoglobulin containing and proline-rich receptor-1 (IGPR-1) is upregulated in human primary colon cancer. Our study demonstrates that IGPR-1 promotes tumor multicellular aggregation, and interfering with its adhesive function inhibits multicellular aggregation and, increases cell death. IGPR-1 supports colon carcinoma tumor xenograft growth in mouse, and inhibiting its activity by shRNA or blocking antibody inhibits tumor growth. More importantly, IGPR-1 regulates sensitivity of tumor cells to the chemotherapeutic agent, doxorubicin/adriamycin by a mechanism that involves doxorubicin-induced AKT activation and phosphorylation of IGPR-1 at Ser220. Our findings offer novel insight into IGPR-1's role in colorectal tumor growth, tumor chemosensitivity, and as a possible novel anti-cancer target.Grant support from: R01 CA175382/CA/NCI NIH HHS/United States, R21 CA191970/CA/NCI NIH HHS/United States, and R21 CA193958/CA/NCI NIH HHS/United State

poolMC: Smart pooling of mRNA samples in microarray experiments

Background: Typically, pooling of mRNA samples in microarray experiments implies mixing mRNA from several biological-replicate samples before hybridization onto a microarray chip. Here we describe an alternative smart pooling strategy in which different samples, not necessarily biological replicates, are pooled in an information theoretic efficient way. Further, each sample is tested on multiple chips, but always in pools made up of different samples. The end goal is to exploit the compressibility of microarray data to reduce the number of chips used and increase the robustness to noise in measurements. Results: A theoretical framework to perform smart pooling of mRNA samples in microarray experiments was established and the software implementation of the pooling and decoding algorithms was developed in MATLAB. A proof-of-concept smart pooled experiment was performed using validated biological samples on commercially available gene chips. Conclusions: The theoretical developments and experimental demonstration in this paper provide a useful starting point to investigate smart pooling of mRNA samples in microarray experiments. Important conditions for its successful implementation include linearity of measurements, sparsity in data, and large experiment size.

Kinematics of subluminous O and B stars by surface helium abundance

The majority of hot subdwarf stars are low-mass core-helium-burning stars. Their atmospheres are generally helium deficient; however, a minority have extremely helium-rich surfaces. An additional fraction have an intermediate surface-helium abundance, occasionally accompanied by peculiar abundances of other elements. We have identified a sample of 88 hot subdwarfs including 38 helium-deficient, 27 intermediate-helium and 23 extreme-helium stars for which radial-velocity and proper-motion measurements, together with distances, allow a calculation of galactic space velocities. We have investigated the kinematics of these three groups to determine whether they belong to similar or different Galactic populations. The majority of helium-deficient subdwarfs in our sample show a kinematic distribution similar to that of thick disc stars. Helium-rich sdBs show a more diverse kinematic distribution. Although the majority are probably disc stars, a minority show a much higher velocity dispersion consistent with membership of a Galactic halo population. Several of the halo subdwarfs are members of the class of ‘heavy-metal’ subdwarfs discovered by Naslim et al

Subaru and salt spectroscopy of chemically peculiar hot subdwarfs

The majority of hot subdwarfs lie on or close to the helium main-sequence. Many have hydrogen-rich surfaces, but a substantial fraction of the hotter subdwarfs have hydrogen-depleted or hydrogen-deficient surfaces. Amongst the former, three were known to show extraordinary overabundances of heavy elements including zirconium and lead. Using Subaru/HDS,we commenced a high-resolution survey of hydrogen-depleted subdwarfs to discover new members of the class. UVO 0825+15, was found to exhibit strong lead lines, to be an intrinsic variable in K2 field 5, and to have a relatively high space motion. Two other lead-rich subdwarfs have been found in the Subaru sample. A much wider survey is in progress using SALT/HRS. Discoveries so far include one extreme helium star similar to V652 Her, and an intermediate helium star with possible comparison to HD144941. Analyses of the hotter and more compact members of the sample are continuing. Open Access

Subaru and SALT spectroscopy of chemically peculiar hot subdwarfs

The majority of hot subdwarfs lie on or close to the helium main-sequence. Many have hydrogen-rich surfaces, but a substantial fraction of the hotter subdwarfs have hydrogen-depleted or hydrogen-deficient surfaces. Amongst the former, three were known to show extraordinary overabundances of heavy elements including zirconium and lead. Using Subaru/HDS,we commenced a high-resolution survey of hydrogen-depleted subdwarfs to discover new members of the class. UVO 0825+15, was found to exhibit strong lead lines, to be an intrinsic variable in K2 field 5, and to have a relatively high space motion. Two other lead-rich subdwarfs have been found in the Subaru sample. A much wider survey is in progress using SALT/HRS. Discoveries so far include one extreme helium star similar to V652 Her, and an intermediate helium star with possible comparison to HD144941. Analyses of the hotter and more compact members of the sample are continuing