5 research outputs found
DataSheet_1_Alleviating psoriatic skin inflammation through augmentation of Treg cells via CTLA-4 signaling peptide.pdf
Psoriasis is a chronic inflammatory skin disease characterized by hyperplasia of keratinocytes and immune cell infiltration. The IL-17-producing T cells play a key role in psoriasis pathogenesis, while regulatory T (Treg) cells are diminished during psoriatic inflammation. Current psoriasis treatments largely focus on IL-17 and IL-23, however, few studies have explored therapeutic drugs targeting an increase of Treg cells to control immune homeostasis. In this study, we investigated the effects of a cytotoxic T lymphocyte antigen-4 (CTLA-4) signaling peptide (dNP2-ctCTLA-4) in Th17, Tc17, γδ T cells, Treg cells in vitro and a mouse model of psoriasis. Treatment with dNP2-ctCTLA-4 peptide showed a significant reduction of psoriatic skin inflammation with increased Treg cell proportion and reduced IL-17 production by T cells, indicating a potential role in modulating psoriatic skin disease. We compared dNP2-ctCTLA-4 with CTLA-4-Ig and found that only dNP2-ctCTLA-4 ameliorated the psoriasis progression, with increased Treg cells and inhibited IL-17 production from γδ T cells. In vitro experiments using a T cell-antigen presenting cell co-culture system demonstrated the distinct mechanisms of dNP2-ctCTLA-4 compared to CTLA-4-Ig in the induction of Treg cells. These findings highlight the therapeutic potential of dNP2-ctCTLA-4 peptide in psoriasis by augmenting Treg/Teff ratio, offering a new approach to modulating the disease.</p
Additional file 1: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer
Raw data of blood analysis from transgenic cattle. (XLSX 15 kb
Additional file 3: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer
Figure S2. Detection of the expression of YFP in milk from SNU-SB-1 by confocal microscopy. Images of milk from wild type cattle (left) and SNU-SB-1 (right) taken using a high-throughput confocal microscope. YFP: YFP field. (PNG 1685 kb
Additional file 4: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer
Figure S3. 5Ⲡjunction sequence analysis of all integration sites in SNU-F1â1. Sequences showing the genome-to-transposon junctions in the genome of SNU-F1â1 and the integration of transgenes at TTAA sites. (PNG 465 kb
Additional file 7: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer
Figure S5. Overview of genomic variation in SNU-F1â2. Reference chromosomes from bt1 to btX are denoted by colored boxes at the outer edge. Plots denoting copy number variation (CNV; black dot plots in the green area), coverage (green line plot in the green area) and SNP density (orange histogram in orange area) for the SNU-F1â2 genome are shown for each 10Â kb window. (PNG 1244 kb