Genomic analysis of a novel antarctic bacterium, cryobacterium sp. SO2 provides insights into its genomic potential for production of antimicrobial compounds

A novel strain of Cryobacterium designated as SO2, was isolated from the Antarctic. Hence, this study was undertaken to gain further insight into the antimicrobial compounds and secondary metabolites produced by Cryobacterium sp. SO2. It was found that strain SO2 is a Gram-positive that exhibits an irregular rod shape, which formed yellow to orange pigmented colonies on semi-solid media. Strain SO2 grows at temperatures ranging from 4 to 25 ºC. It has a complete genomic size of 4.097 Mb. SO2 has a DNA G+C content of 68.43%, and genomic annotation showed that the genome contained 3,862 CDS, 10 rRNA, 55 tRNA and 1 tm-RNA. Phylogenetic and OrthoANI analysis suggested Cryobacterium sp. strains SO1, N22, TMB1-8, LW097, TMN39-1, C. zongtaii TMN-42, C. arcticum PAMC27867 and C. soli GCJ02 as its closest phylogenetic neighbour. Genome annotation shows that strain SO2 confers β-lactamase class A, cephalosporin-C deacetylases, and 27 drug-resistance or efflux coding genes, and allows resistance to ceftazidime. Functional annotation identifies 28.74% of predicted genes are of unknown functions. Genome mining indicates that there are six putative secondary metabolite gene clusters in strain SO2. They are made up of RRE-containing, terpene, beta-lactone, T3PKS, NAPAA, and 2dos. This finding shows strain SO2 harbours genes that may be involved in the production of compounds with antibacterial and antioxidant activities

Draft genome sequence of antarctic psychrotroph Streptomyces fildesensis strain INACH3013, isolated from King George Island soil

The draft genome sequence of Streptomyces fildesensis strain INACH3013, a psychrotrophic bacterium isolated from Northwest Antarctic soil, was reported. The genome sequence totaling 9,306,785 bp resulted from 122 contigs characterized by a GC content of 70.55%

Isolation and characterization of Antarctic psychrotroph Streptomyces sp. strain INACH3013

An actinobacterial strain with antimicrobial activity, INACH3013, was isolated from soil collected from Antarctica. The taxonomic status of the isolate was established using a polyphasic approach. The strain was identified as belonging to the genus Streptomyces based on the scanning electron microscopic observation and partial 16S rRNA gene sequence analysis. The sequence analysis revealed that strain INACH3013 is closely related to Streptomyces fildesensis (99.8%), S. beijiangensis (98.1%) and S. purpureus (97.2%). A phylogenetic tree constructed using the partial 16S rRNA gene sequences of strain INACH3013 and closely related strains revealed that INACH3013 fell into the same subclade as S. fildesensis and S. purpureus. Strain INACH3013 was observed to be psychrotolerant, slightly halotolerant (up to 5% NaCl) and capable of inhibiting the growth of seven Gram-negative and eight Gram-positive foodborne pathogens. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain is KJ624755