Monthly percent of <i>A</i>. <i>agrarius</i> captured at Nightmare Range¹, by weight, from January 2008-December 2009.

<p>¹ One each of <i>Micromys minutus</i> and <i>Myodes regulus</i> were seropositive for hantaviruses during January 2009 when the hantavirus seropositive rate for <i>Apodemus agrarius</i> was 5.1%. One <i>Crocidura lasiura</i> was seropositive for Imjin virus during June.</p

Proportion of male and female <i>A</i>. <i>agrarius</i>, by weight category, that were seropositive for hantaviruses, Nightmare Range, January 2008-December 2009.

<p>Proportion of male and female <i>A</i>. <i>agrarius</i>, by weight category, that were seropositive for hantaviruses, Nightmare Range, January 2008-December 2009.</p

Number of <i>Apodemus agrarius</i> females captured monthly and percent gravid at Nightmare Range from January 2008-December 2009.

<p>Number of <i>Apodemus agrarius</i> females captured monthly and percent gravid at Nightmare Range from January 2008-December 2009.</p

Maximum likelihood method based on a 320-nt region of the G_C glycoprotein-encoding M segment of the HTNV amplified from 12 <i>A</i>. <i>agrarius</i> captured at Nightmare Range (GenBank accession numbers; KM279662-KM279673).

<p>PC stands for Pocheon, and YC stands for Yeoncheon. HTNV 76–118, Lee, Hojo, and Bao 14 are included for comparison. GenBank accession numbers for HTN 76–118, HTN Lee, HTN HoJo, and HTN Bao14 are M14627, D00377, D00376, and AB127995, respectively. Branch lengths are proportional to the number of nucleotide substitutions, while vertical distances are for clarity only. The numbers at each node are bootstrap probabilities (expressed as percentages), as determined for 1000 iterations by MEGA6.0.</p

Monthly percent of male, female, and mean (line) A.

<p><b>agrarius seropositive for hantaviruses from January 2008-December 2009.</b> A total of three, one each <i>Micromys minutus</i>, <i>Myodes regulus</i>, and <i>Crocidura lasiura</i>, were seropositive for hantaviruses (not included).</p

Nightmare Range, Gyeonggi Province, where rodent-borne disease surveillance was conducted monthly from January 2008-December 2009.

<p>Nightmare Range, Gyeonggi Province, where rodent-borne disease surveillance was conducted monthly from January 2008-December 2009.</p

Hantavirus surveillance and genetic diversity targeting small mammals at Camp Humphreys, a US military installation and new expansion site, Republic of Korea

<div><p>Small mammal surveillance was conducted (2008–2010, 2012) at Camp (Cp) Humphreys, a US Army installation and new expansion site, Republic of Korea (ROK), to identify hemorrhagic fever with renal syndrome health threats to US military/civilian populations during its ongoing expansion phase. Small mammals were collected using Sherman live capture traps and transported to Korea University where they were euthanized, tissues removed, and assayed to determine hantavirus IgG antibody-positive and hantavirus-positive rates by RT-PCR. A total of 2,364 small mammals were captured over 11,300 trap nights (capture rate = 20.92%). <i>Apodemus agrarius</i> was the most commonly collected (76.65%), with capture rates of 9.62% and 21.70% for Cp Humphreys and the expansion site, respectively. Overall, Hantaan virus (HTNV) IgG antibody-positive (Ab+) rate for <i>A</i>. <i>agrarius</i> was 2.15% (39/1,812). A total of 5.43% (10/184) <i>Crocidura lasiura</i>, 0.79% (2/254) <i>Microtus fortis</i> and 2.44% (1/41) <i>Micromys minutus</i> were serologically IgG Ab+ for hantaviruses. HTNV-specific RT-PCR demonstrated that 28.2% (11/39) HTNV Ab+ <i>A</i>. <i>agrarius</i> harbored the 328-nt sequence of the G_C glycoprotein-encoding M segment of HTNV. Among them, the whole genome sequences of 3 HTNV strains were obtained by conventional RT-PCR and Rapid Amplification cDNA Ends PCR. Phylogenetic analyses of the HTNV strains from Cp Humphreys and the expansion site, Pyeongtaek, show a greater diversity of rodent-borne hantaviruses compared to HTNV previously identified in Gyeonggi province of the ROK. Thus, this study provides significant insights for raising HFRS threat awareness, analysis, and risk reduction strategies in southern Gyeonggi province.</p></div

Overall monthly percentage of male and female <i>Apodemus agrarius</i> captured at Camp Humphreys and the new expansion site that were antibody-positive for Hantaan virus, 2008–2010 and 2012.

<p>Overall monthly percentage of male and female <i>Apodemus agrarius</i> captured at Camp Humphreys and the new expansion site that were antibody-positive for Hantaan virus, 2008–2010 and 2012.</p

Number, capture rate, and percent of small mammals captured, by species, at both Camp Humphreys and the expansion site (under development), monthly 2008–2009, quarterly 2010, and biannually 2012.

<p>Number, capture rate, and percent of small mammals captured, by species, at both Camp Humphreys and the expansion site (under development), monthly 2008–2009, quarterly 2010, and biannually 2012.</p

The total number and percentage of male and female <i>A</i>. <i>agrarius</i> captured, by weight category, mean weights for each category, and differences of mean male weights—Mean female weights for each weight category during 2008–2010 and 2012.

<p>The total number and percentage of male and female <i>A</i>. <i>agrarius</i> captured, by weight category, mean weights for each category, and differences of mean male weights—Mean female weights for each weight category during 2008–2010 and 2012.</p