Progression from Chronic Atrophic Gastritis to Gastric Cancer; Tangle, Toggle, Tackle with Korea Red Ginseng

Key molecular players that link inflammation to carcinogenesis are prostaglandins, cytokines, nuclear factor-κB (NF-κB), chemokines, angiogenic growth factors, and free radicals, all of which lead to increased mutations and altered functions of important enzymes and proteins, for example, activation of oncogenic products and/or inhibition of tumor suppressor proteins, in inflamed tissues, thus contributing to multi-stage carcinogenesis process. Interpreted reversely, the identification of the molecular mechanisms by which chronic inflammation increases cancer risk or optimal intervention of targeted drugs or agents during the inflammation-associated carcinogenic process could be a necessary basis for developing new strategy of cancer prevention at many sites. In this review, we discuss the possibilities for cancer prevention by controlling inflammation process in Helicobacter pylori (H. pylori)-associated inflamed stomach with Korea red ginseng. Korea red ginseng is a good example of a natural herb that has ubiquitous properties that are conductive to stop inflammatory carcinogenesis that is un wanted outcome of H. pylori infection, rendering rejuvenation of chronic atrophic gastritis

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

Live cell imaging of mammalian RNA polymerase II (Pol II) has previously relied on random insertions of exogenous, mutant Pol II coupled with the degradation of endogenous Pol II using a toxin, α-amanitin. Therefore, it has been unclear whether over-expression of labeled Pol II under an exogenous promoter may have played a role in reported Pol II dynamics in vivo. Here we label the endogenous Pol II in mouse embryonic fibroblast (MEF) cells using the CRISPR/Cas9 gene editing system. Using single-molecule based super-resolution imaging in the living cells, we captured endogenous Pol II clusters. Consistent with previous studies, we observed that Pol II clusters were short-lived (cluster lifetime ~8 s) in living cells. Moreover, dynamic responses to serum-stimulation, and drug-mediated transcription inhibition were all in agreement with previous observations in the exogenous Pol II MEF cell line. Our findings suggest that previous exogenously tagged Pol II faithfully recapitulated the endogenous polymerase clustering dynamics in living cells, and our approach may in principle be used to directly label transcription factors for live cell imaging.National Cancer Institute (U.S.) (Award DP2CA195769)Massachusetts Institute of Technology. Department of Physic

Laparoscopy-assisted distal gastrectomy for early gastric cancer and laparoscopic cholecystectomy for gallstone with situs inversus totalis: a case report

We report our case of laparoscopy-assisted distal gastrectomy with D1 + β lymph node dissection for a patient with early gastric cancer and laparoscopic cholecystectomy for gallstone with situs inversus totalis. A superficial elevated lesion was found on the lesser curvature of the antrum. The preoperative diagnosis was cStage IA (cT1, cN0, cH0, cP0, cM0). A 1 cm-sized gallstone was found in the fundus through upper abdominal ultrasound. A laparoscopy-assisted distal gastrectomy with standard D2 lymph node dissection for early gastric cancer and laparoscopic cholecystectomy was successfully performed by not shifting the monitor to the left and right and not changing operator's position without additional blood loss and time. The number of retrieved lymph nodes was 36. We have not found any abnormal course of blood vessels except for the right/left inversion. Billroth I reconstruction was performed through end-to-side anastomosis. Based on a histopathological examination, a 1.5 × 1.5 cm, submucosal (sm3), moderately differentiated adenocarcinoma (pT1, pN0, sH0, sP0, sM0, stage IA) was diagnosed. The postoperative course was favorable and the patient was discharged on postoperative day 7

Solitary Necrotic Nodules of the Liver Mimicking Hepatic Metastasis: Report of Two Cases

We present two cases of solitary necrotic nodules of the liver which on radiologic images mimicked hepatic metastasis. Solitary necrotic nodule of the liver is a rare but benign entity which histopathologically consists of an outer fibrotic capsule with inflammatory cells and a central core of amorphous necrotic material. The lesion was seen on contrast-enhanced CT as an ovoid-shaped hypoattenuating nodule; on CT during hepatic arteriography as enhancing nodule; on intraoperative US as a target-appearing hypoechoic nodule; on T2WI as a hyperintensity nodule, and on dynamic MR as a subtle peripheral enhancing nodule. Although the radiologic features are not specific, solitary necrotic nodule of the liver should be included in the differential diagnosis of hepatic metastasis

Corrugated Silicon Platelet Feed Horn Array for CMB Polarimetry at 150 GHz

Next generation cosmic microwave background (CMB) polarization anisotropy measurements will feature focal plane arrays with more than 600 millimeter-wave detectors. We make use of high-resolution photolithography and wafer-scale etch tools to build planar arrays of corrugated platelet feeds in silicon with highly symmetric beams, low cross-polarization and low side lobes. A compact Au-plated corrugated Si feed designed for 150 GHz operation exhibited performance equivalent to that of electroformed feeds: ~-0.2 dB insertion loss, <-20 dB return loss from 120 GHz to 170 GHz, <-25 dB side lobes and <-23 dB cross-polarization. We are currently fabricating a 50 mm diameter array with 84 horns consisting of 33 Si platelets as a prototype for the SPTpol and ACTpol telescopes. Our fabrication facilities permit arrays up to 150 mm in diameter.Comment: 12 pages; SPIE proceedings for Millimeter, Submillimeter, and Far-Infrared Detectors and Instrumentation for Astronomy V (Conference 7741, June 2010, San Diego, CA, USA

Hearing Abilities at Ultra-High Frequency in Patients with Tinnitus

ObjectivesTo compare tinnitus patients who have normal hearing between 250 Hz and 8 kHz with normal controls with regard to the ability of each group to hear extended high-frequency pure tone thresholds.MethodsWe enrolled 18 tinnitus patients, each of whom had a threshold of HL <25 dB and threshold differences of <10 dB between ears at frequencies of 250 and 500 Hz and 1, 2, 4, and 8 kHz. We also enrolled age- and gender-matched normal volunteers (10 ears), for each patient. Extended high frequency pure tone audiometry was performed, and the mean hearing thresholds at 10, 12, 14, and 16 kHz of each tinnitus ear were compared with those of the 10 age- and sex-matched normal ears.ResultsOf the 18 patients with tinnitus, 12 had significantly increased hearing thresholds at more than one of the four high frequencies, compared with the normal group. When we assessed results according to frequency, we found that 8 patients had decreased hearing ability at 10 kHz, 10 at 12 kHz, 8 at 14 kHz, and 4 at 16 kHz.ConclusionSome patients with tinnitus who have normal hearing below 8 kHz have decreased hearing ability at extended high-frequencies. Thus, the proportion of patients with tinnitus who have normal hearing over the entire audible range is smaller than in previous reports

Confirmation of the performance of exfoliated graphite nanoplatelets for pollutant reduction rate on wood panel

Abstract Exfoliated graphite nanoplatelets have been used as an additive for the improvement of a variety of properties. Nowadays, it is used as an adsorbent for pollutants, especially volatile organic compounds and formaldehyde. Additionally, exfoliated graphite nanoplatelets have been used as an additive in wood composites, because wood composites have a higher thermal conductivity than timber or typical wood-based panels. Therefore, exfoliated graphite nanoplatelets have been applied to high-density fiberboard which is a core material of wood flooring for the emission rate reduction of volatile organic compounds and formaldehyde and for increasing the thermal conductivity of wood flooring in radiant floor heating systems. Volatile organic compound emissions have been reduced significantly in wood composite boards according to the addition of exfoliated graphite nanoplatelets. The properties of formaldehyde emission and thermal conductivity decreased when 2% exfoliated graphite nanoplatelets are added to high-density fiberboard. However, these properties increased with the increase in the addition of exfoliated graphite nanoplatelets from 2% to 4%

Microlensing Optical Depth Revisited with Recent Star Counts

More reliable constraints on the microlensing optical depth comes from a better understanding of the Galactic model. Based on well-constrained Galactic bulge and disk models constructed from survey observations, such as, HST, 2MASS, and SDSS, we calculate the microlensing optical depths toward the Galactic bulge fields, and compare them with recent results of microlensing surveys. We test chi^2 statistics of microlensing optical depths expected from those models, as well as previously proposed models, using two types of data: optical depth map in (l, b) and averaged optical depth over the Galactic longitude l as a function of the latitude b. From this analysis, we find that the Galactic bulge models of 2MASS, Han & Gould (2003), and G2 of Stanek et al. (1997) show a good agreement with the microlensing optical depth profiles for all the microlensing observations, compared with E2 of Stanek et al. (1997). We find, on the other hand, that models involving an SDSS disk model produce relatively higher chi^2 values. It should be noted that modeled microlensing optical depths diverge in the low Galactic latitude, |b| < 2 arcdeg. Therefore, we suggest the microlensing observation toward much closer to central regions of the Galaxy to further test the proposed Galactic models, if it is more technically feasible than waiting for large data set of microlensing events.Comment: 15 pages, 3 figures and 3 tables, accepted for publication in Ap