Continuous detection of viable micro-organisms by chemiluminescence

System monitors quality of reclaimed water continuously and automatically. Incubated samples are compared with unincubated ones by measuring their respective chemiluminescence

Automated monitoring of recovered water quality

Laboratory prototype water quality monitoring system provides automatic system for online monitoring of chemical, physical, and bacteriological properties of recovered water and for signaling malfunction in water recovery system. Monitor incorporates whenever possible commercially available sensors suitably modified

Entrainment of sediment particles by very-large-scale motions

Acknowledgements The study has been supported by two EPSRC/UK grants, ‘High-resolution numerical and experimental studies of turbulence-induced sediment erosion and near-bed transport’ (EP/G056404/1) and ‘Bed friction in rough-bed free-surface flows: a theoretical framework, roughness regimes, and quantification’ (EP/K041088/1). The authors are grateful to three anonymous reviewers and the Editor for constructive criticisms and helpful suggestions that improved the presentation of the material in the paper.Peer reviewedPostprin

A novel experimental technique and its application to study the effects of particle density and flow submergence on bed particle saltation

This research was sponsored by EPSRC grant EP/G056404/1 which is greatly appreciated.Peer reviewedPublisher PD

The social geography of childcare: 'making up' the middle class child

Childcare is a condensate of disparate social forces and social processes. It is gendered and classed. It is subject to an excess of policy and political discourse. It is increasingly a focus for commercial exploitation. This is a paper reporting on work in progress in an ESRC funded research project (R000239232) on the choice and provision of pre-school childcare by middle class (service class) families in two contrasting London locations. Drawing on recent work in class analysis the paper examines the relationships between childcare choice, middle class fractions and locality. It suggests that on the evidence of the findings to date, there is some evidence of systematic differences between fractions in terms of values, perspectives and preferences for childcare, but a more powerful case for intra-class similarities, particularly when it comes to putting preferences into practice in the 'making up of a middle class child' through care and education

Camp Lwandle: Rehabilitating a migrant labour hostel at the seaside

In southern African narratives of migrant labour, hostels and compounds are represented as typical examples of colonial and apartheid planning. Visual and spatial comparisons are consistently made between the regulatory power of hostels and those of concentration camps. Several of these sites of violence and repression are today being reconfigured as sites of conscience, their artefactual presence on the landscape being constructed as places of remembrance. In this trajectory, a space of seeming anonymity in Lwandle, some 40 km outside of Cape Town, was identified by the newly established museum, at the beginning of the twenty-first century, as a structure of significance. The migrant labour compound in Lwandle, of which Hostel 33 is the last remnant, was designed by planners and engineers and laid out as part of a labour camp for male migrant workers in the 1950s. This article explores the ambitious project initiated in 2008, by the Lwandle Migrant Labour Museum (and funded largely by the US Ambassadors Cultural Restoration Fund), to restore Hostel 33. Although Hostel 33 was not a very old structure, having been built in 1958/9, nor was it easily considered to have conventional architectural significance, its material presence in present-day Lwandle represents a reminder of the conditions of life in the labour camp. The article traces the work entailed in the restoration process through paying attention to both the built fabric and its materiality, and by giving an account of the explorations into finding ways to restore the hostel to the museum through making it into a site of significance. In place of the centrality of the building as the object of restoration, the work shifted to considering how the hostel could function most effectively as a stage and destination for the Museum’s narrations of the past. Retaining and maintaining Hostel 33 was less concerned with the fabric as an empirical fact of the past, than with its projection into an envisaged future for museum purposes.Department of HE and Training approved lis

The association of the long prostate cancer expressed PDE4D transcripts to poor patient outcome depends on the tumor’s TMPRSS2-ERG fusion status

Objectives: To investigate the added value of assessing transcripts for the long cAMP phosphodiesterase-4D (PDE4D) isoforms, PDE4D5 and PDE4D9, regarding the prognostic power of the ‘CAPRA & PDE4D7’ combination risk model to predict longitudinal postsurgical biological outcomes in prostate cancer. Patients and Methods: RNA was extracted from both biopsy punches of resected tumours (606 patients; RP cohort) and diagnostic needle biopsies (168 patients; DB cohort). RT-qPCR was performed in order to determine PDE4D5, PDE4D7, and PDE4D9 transcript scores in both study cohorts. By RNA sequencing, we determined the TMPRSS2-ERG fusion status of each tumour sample in the RP cohort. Kaplan-Meier survival analyses were then applied to correlate the PDE4D5, PDE4D7 and PDE4D9 scores with postsurgical patient outcomes. Logistic regression was then used to combine the clinical CAPRA score with PDE4D5, PDE4D7, and PDE4D9 scores in order to build a ‘CAPRA & PDE4D5/7/9’ regression model. ROC and decision curve analysis was used to estimate the net benefit of the ‘CAPRA & PDE4D5/7/9’ risk model. Results: Kaplan-Meier survival analysis, on the RP cohort, revealed a significant association of the PDE4D7 score with postsurgical biochemical recurrence (BCR) in the presence of the TMPRSS2-ERG gene rearrangement (logrank p<0.0001), compared to the absence of this gene fusion event (logrank p=0.08). In contrast, the PDE4D5 score was only significantly associated with BCR in TMPRSS2-ERG fusion negative tumours (logrank p<0.0001 vs. logrank p=0.4 for TMPRSS2-ERG+ tumours). This was similar for the PDE4D9 score although less pronounced compared to that of the PDE4D5 score (TMPRSS2ERG- logrank p<0.0001 vs. TMPRSS2ERG+ logrank p<0.005). In order to predict BCR after primary treatment, we undertook ROC analysis of the logistic regression combination model of the CAPRA score with the PDE4D5, PDE4D7, and PDE4D9 scores. For the DB cohort, this demonstrated significant differences in the AUC between the CAPRA and the PDE4D5/7/9 regression model vs. the CAPRA and PDE4D7 risk model (AUC 0.87 vs. 0.82; p=0.049) vs. the CAPRA score alone (AUC 0.87 vs. 0.77; p=0.005). The CAPRA and PDE4D5/7/9 risk model stratified 19.2% patients of the DB cohort to either ‘no risk of biochemical relapse’ (NPV 100%) or the ‘start of any secondary treatment (NPV 100%)’, over a follow-up period of up to 15 years. Decision curve analysis presented a clear, net benefit for the use of the novel CAPRA & PDE4D5/7/9 risk model compared to the clinical CAPRA score alone or the CAPRA and PDE4D7 model across all decision thresholds. Conclusion: Association of the long PDE4D5, PDE4D7, and PDE4D9 transcript scores to prostate cancer patient outcome, after primary intervention, varies in opposite directions depending on the TMPRSS2-ERG genomic fusion background of the tumour. Adding transcript scores for the long PDE4D isoforms, PDE4D5 and PDE4D9, to our previously presented combination risk model of the combined ‘CAPRA & PDE4D7’ score, in order to generate the CAPRA and PDE4D5/7/9 score, significantly improves the prognostic power of the model in predicting postsurgical biological outcomes in prostate cancer patients