Tolerância ao frio e caracterização de híbridos entre leucaena leucocephala e L. diversifolia Cold tolerance and characterization of hybrids between leucaena leucocephala and L. diversifolia

O objetivo deste experimento foi identificar material promissor para ser utilizado como uma forrageira alternativa nas condições do sul do Brasil. Uma população de híbridos, em gerações avançadas, entre Leucaena leucocephala e L. diversifolia (ambas espécies tetraplóides, com 2n=104 cromossomos) foi avaliada quanto à taxa de crescimento em altura, época de florescimento e frutificação, número de flores e de legumes por inflorescência e de sementes por legume, tolerância ao frio (expressa em retenção de folhas durante o inverno) e capacidade de rebrote após o inverno. Foi detectada grande variabilidade intrapopulacional para todas as características avaliadas. Foram identificados indivíduos, reunindo tolerância a frio com características desejáveis, que podem ser utilizados na continuidade dos trabalho de seleção e melhoramento e comprovam a potencialidade de Leucaena como espécie forrageira para o sul do Brasil.<br>The objective of this experiment was to identify material to be used as an alternative forage in the conditions of Southern Brazil. A population of hybrids between L. leucocephala and L. diversifolia (both tetraploid species, with 2n=104 chromosomes) in advanced generations was evaluated. The rate of growth in height, duration of flowering and frutification, number of flowers and legumes per inflorescence, seeds per legume, cold tolerance (expressed as foliage retention during winter) and regrowth ability in early spring were evaluated. A great intrapopulational variability was detected for all evaluated characteristics. Some individuals allying cold tolerance and desirable characteristics were identified to be used for further selection and breeding, which confirms the potentiality of Leucaena as a forage for Southern Brazil

Fetofetal transfusion syndrome: do the neonatal criteria apply in utero?

Thirteen fetuses (five twin, one triplet) were compromised by fetofetal transfusion syndrome in six pregnancies, five in the mid trimester, and one in the third trimester. This diagnosis, which was suspected because of ultrasound findings of discordant growth, discordant amniotic fluid volumes, concordant external genitalia, and monochorial placentation, was confirmed postnatally in each. Nine fetuses underwent blood sampling to aid diagnosis and assessment of fetal wellbeing. In contrast to fetofetal transfusion syndrome investigated postnatally, a difference in haemoglobin concentration of 50 g/l or more in utero was found in only one pregnancy, which was near term, although all had fetal erythroblastaemia and a difference in weight of 20% or more. In vivo confirmation of shared circulation was achieved in two pregnancies by transfusing adult Rh negative red cells into the smaller fetus and then detecting them by Kleihauer testing in blood aspirated from the larger. Invasive procedures also yielded information on fetal blood gas measurements (acidaemia in four and hypoxaemia in six) and amniotic pressure (raised in two). We suggest that comparison of haemoglobin concentrations is inaccurate in fetofetal transfusion syndrome in utero, the diagnosis of which may necessitate detection of a shared circulation using a marker such as adult red cells