30 research outputs found
An Analytical Framework to Describe the Interactions Between Individuals and a Continuum
We consider a discrete set of individual agents interacting with a continuum.
Examples might be a predator facing a huge group of preys, or a few shepherd
dogs driving a herd of sheeps. Analytically, these situations can be described
through a system of ordinary differential equations coupled with a scalar
conservation law in several space dimensions. This paper provides a complete
well posedness theory for the resulting Cauchy problem. A few applications are
considered in detail and numerical integrations are provided
The ATPases of Chlamydomonas outer arm dynein differ in their pH and cationic requirements
International audienc
The ATPases of Chlamydomonas outer arm dynein differ in thei pH and cationic requirements
International audienc
The ATPases of Chlamydomonas outer arm dynein differ in their pH and cationic requirements
International audienc
Outer-arm dynein from trout spermatozoa : substructural organization
International audienc
Outer-arm dynein from trout spermatozoa : substructural organization
International audienc
The Outer Dynein Arm-Docking Complex: Composition and Characterization of a Subunit (Oda1) Necessary for Outer Arm Assembly
To learn more about how dyneins are targeted to specific sites in the flagellum, we have investigated a factor necessary for binding of outer arm dynein to the axonemal microtubules of Chlamydomonas. This factor, termed the outer dynein arm-docking complex (ODA-DC), previously was shown to be missing from axonemes of the outer dynein armless mutants oda1 and oda3. We have now partially purified the ODA-DC, determined that it contains equimolar amounts of M(r) ∼105,000 and ∼70,000 proteins plus a third protein of M(r) ∼25,000, and found that it is associated with the isolated outer arm in a 1:1 molar ratio. We have cloned a full-length cDNA encoding the M(r) ∼70,000 protein; the sequence predicts a 62.5-kDa protein with potential homologs in higher ciliated organisms, including humans. Sequencing of corresponding cDNA from strain oda1 revealed it has a mutation resulting in a stop codon just downstream of the initiator ATG; thus, it is unable to make the full-length M(r) ∼70,000 protein. These results demonstrate that the ODA1 gene encodes the M(r) ∼70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule