Pressure dependent diameter of female and male anterior inferior cerebellar artery.

<p>Original trace of female (A) and male (B) anterior inferior cerebellar artery in 10 µM L-NNA and Ca²⁺-free solution. Diameter in % in 10 µM L-NNA and Ca²⁺-free solution (C), % values were normalized to maximal diameter measured in Ca²⁺-free at 90 cmH₂O. 100% = 134.6 µm±6 in Ca²⁺-free at 90 cmH₂O in female animals and 132.8 µm±7.6 in Ca²⁺-free at 90 cmH₂O in male animals.</p

Calculated flow rate for brain arteries from rat and gerbil and cochlea arteries from gerbil.

<p>Male rat mid-cerebral artery (A) taken from Geary et al 1998 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025659#pone.0025659-Geary1" target="_blank">[19]</a>, male gerbil basilar artery (B) male gerbil anterior inferior cerebellar artery (C), female rat mid-cerebral artery territory branch (D), male gerbil spiral modiolar artery (E), male gerbil radiating arteriole (F). Green bars represent estimated physiological pressure range for each vessel according to which active and passive slope were calculated. Slope is given in µl/min –cmH₂O. For active slope diameter was obtained in 10 µM L-NNA, for passive slope diameter was obtained in Ca²⁺-free solution.</p

Myogenic tone development along the cochlear vascular tree in male and female gerbil.

<p>The myogenic tone at the reference pressure of 60 cmH₂O was 4±2% in male (n = 5) and 4±1% in female (n = 4) basilar arteries (A), 7±1% in male (n = 6) and 4±1% in female (n = 7) anterior inferior cerebellar arteries (B), 17±4% in male (n = 6) and 6±3% in female (n = 7) spiral modiolar arteries (C), and 16±2% in male (n = 4) and 15±3% in female (n = 4) radiating arterioles (D). “*” indicates statistical significance (p<0.05) by ANOVA (2-factor repeated-measures).</p

Diameter and myogenic tone in female rat mid-cerebral artery territory branch.

<p>Original trace of female rat mid-cerebral artery territory branch in 10 µM L-NNA and Ca²⁺-free solution (A). Calculated myogenic tone in %, n = 1 (B).</p

Pressure dependent diameter of female and male radiating arteriole.

<p>Original trace of female (A) and male (B) radiating arteriole in 10 µM L-NNA and Ca²⁺-free solution. Diameter in % in 10 µM L-NNA and Ca²⁺-free solution (C), % values were normalized to maximal diameter measured in Ca²⁺-free at 80 cmH₂O. 100% = 52.4 µm±3.3 in Ca²⁺-free at 90 cmH₂O in female animals and 53.2 µm±1.7 in Ca²⁺-free at 90 cmH₂O in male animals.</p

Pressure dependent diameter of female and male basilar artery.

<p>Original traces of female (A) and male (B) basilar artery in 10 µM L-NNA and Ca²⁺-free solution. Diameter in % in 10 µM L-NNA and Ca²⁺-free solution (C), % values were normalized to maximal diameter measured in Ca²⁺-free at 90 cmH₂O. 100% = 329.2 µm±17.5 in Ca²⁺-free at 90 cmH₂O in female animals and 313.8 µm±7.7 in Ca²⁺-free at 90 cmH₂O in male animals.</p

Schematic drawing of the blood vessels supplying the cochlea.

<p>Basilar artery and anterior inferior cerebellar artery ventral of the gerbil brain stem (A). Cochlea with spiral modiolar artery and radiating arteriole (B).</p

Pressurization of vessels using the concentric pipette system.

<p>Schematic drawing of pressurization system (A) showing the vessel at the right end linked to a concentric pipette system with a hydrostatic pressure column of variable height to control transmural pressure while the left end of the vessel is occluded by pressing the vessel against the bottom of the bath chamber with a plan glass holder. Pressurized basilar artery (B), anterior inferior cerebellar artery (C), spiral modiolar artery (D), and radiating arteriole (E), scale bars = 50 µm.</p