Developments in plant breeding for improved nutritional quality of soya beans I. Protein and amino acid content

Soya beans, like other legumes, contain low concentrations of the nutritionally essential sulphur amino acid, methionine. Cysteine, although not an essential amino acid because it can be synthesized from methionine, also influences the nutritional quality of soya bean products when it is only present in low levels. A low cysteine content will also aggravate a methionine deficiency. Soya bean lines deficient in 7S protein subunits have been identified. The 7S proteins contain substantially less methionine and cysteine than the 11S proteins. With the myriad of genetic null alleles for these subunits it may be possible to tailor the 7S/11S storage protein ratio and their total composition in seeds to include only those subunits with the richest sulphur amino acid composition. Cotyledon feeding experiments, using isolated soya bean cotyledons, demonstrated that addition of methionine to the culture media caused increased synthesis of both proteins and free amino acids but the mechanism by which this takes place is not clear

Developments in plant breeding for improved nutritional quality of soya beans II. Anti-nutritional factors

Nutritional value of most plant materials is limited by the presence of numerous naturally occurring compounds which interfere with nutrient digestion and absorption. Although processing is employed widely in removal of these factors, selection of cultivars of soya beans with inherently low levels would have a considerable impact on efficiency of non-ruminant livestock production. The review considers the role of plant breeding in achieving this objective. The most abundant trypsin inhibitors are the Kunitz and the Bowman Birk inhibitors, containing 181 and 71 amino acids respectively. The Kunitz inhibitor is present at a concentration of 1.4g/kg of total seed contents and the Bowman Birk inhibitor 1.6g/kg. A large number of isoforms of the Bowman Birk inhibitor have been described in soya bean cultivars and it has been shown that the general properties of the inhibitor are, in fact, attributable to different isoforms. Nulls for both Bowman-Birk and Kunitz trypsin inhibitors have been identified, allowing new low trypsin inhibitor cultivars to be produced. However, research into breeding for low trypsin inhibitor cultivars currently has limited application as trypsin inhibitors contribute a major proportion of the methionine content of soya beans. Trypsin inhibitors are thought to be involved in the regulation of and protection against unwanted proteolysis in plant tissues and also act as a defense mechanism against attack from diseases, insects and animals. Hence, in breeding programes for low trypsin inhibitor cultivars, alternative protection for growing plants must be considered. Use of soya beans in non-ruminant animal feeds is limited by the flatulence associated with their consumption

Delegation and Positive-Sum Bureaucracies

I develop a formal model of bureaucratic policymaking to investigate why a legislature would choose to delegate authority to a bureaucratic agency whose actions can be controlled, ex post , by an executive with divergent policy preferences. Because the executive and legislature might find different policies to be salient to their constituencies, I demonstrate that executive review of agency rulemaking can benefit both branches of government, relative to legislative delegation without the possibility of such review. In trying to undermine the impacts of executive oversight, agencies propose policies that could benefit the legislature were the executive to choose not to intervene in agency policymaking. Likewise, if the executive does intervene, executive review allows him to implement a policy more desirable than absent such review. This joint-desirability of executive review is more likely when legislative and executive policy preferences are relatively aligned, and when legislative and agency policy preferences are relatively divergent. The broader social welfare consequences of executive review depend on the relative effectiveness of the executive's oversight of agency policymaking. These results provide insight for why mediating lawmaking institutions such as the Office of Information and Regulatory Analysis (OIRA) continue to survive in a separation of powers system despite their potential to advantage one branch of government at the expense of the other.

Phase measurements at the theoretical limit

It is well known that the result of any phase measurement on an optical mode made using linear optics has an introduced uncertainty in addition to the intrinsic quantum phase uncertainty of the state of the mode. The best previously published technique [H. M. Wiseman and R.B. Killip, Phys. Rev. A 57, 2169 (1998)] is an adaptive technique that introduces a phase variance that scales as n^{-1.5}, where n is the mean photon number of the state. This is far above the minimum intrinsic quantum phase variance of the state, which scales as n^{-2}. It has been shown that a lower limit to the phase variance that is introduced scales as ln(n)/n^2. Here we introduce an adaptive technique that attains this theoretical lower limit.Comment: 9 pages, 5 figures, updated with better feedback schem

Quantum error correction for continuously detected errors

We show that quantum feedback control can be used as a quantum error correction process for errors induced by weak continuous measurement. In particular, when the error model is restricted to one, perfectly measured, error channel per physical qubit, quantum feedback can act to perfectly protect a stabilizer codespace. Using the stabilizer formalism we derive an explicit scheme, involving feedback and an additional constant Hamiltonian, to protect an ($n-1$)-qubit logical state encoded in $n$ physical qubits. This works for both Poisson (jump) and white-noise (diffusion) measurement processes. In addition, universal quantum computation is possible in this scheme. As an example, we show that detected-spontaneous emission error correction with a driving Hamiltonian can greatly reduce the amount of redundancy required to protect a state from that which has been previously postulated [e.g., Alber \emph{et al.}, Phys. Rev. Lett. 86, 4402 (2001)].Comment: 11 pages, 1 figure; minor correction

Nuclear mutations affecting mitochondrial structure and function in Chlamydomonas

Wild type cells of the green alga Chlamydomonas reinhardtii can grow in the in the dark by taking up and respiring exogenously supplied acetate. Obligate photoautotrophic (dark dier, dk) mutants of this alga have been selected which grow at near wild type rates in the light, but rapidly die when transferred to darkness because of defects in mitochondrial structure and function. In crosses of the dk mutants to wild type, the majority of the mutants are inherited in a mendelian fashion, although two have been isolated which are inherited in a clearly nonmendelian fashion. Nine mendelian dk mutants have been analyzed in detail, and belong to eight different complementation groups representing eight gene loci. These mutants have been tentatively grouped into three classes on the basis of the pleiotropic nature of their phenotypic defects. Mutants in Class I have gross alterations in the ultrastructure of their mitochondrial inner membranes together with deficiencies in cytochrome oxidase and antimycin/rotenone-sensitive NADH-cytochrome c reductase activities. Mutants in Class II have a variety of less severe alterations in mitochondrial ultrastructure and deficiencies in cytochrome oxidase activity. Mutants in Class III have normal or near normal mitochondrial ultrastructure and reduced cytochrome oxidase activity. Eight of the nine mutants show corresponding reductions in cyanide-sensitive respiration