Estimation of Genetic Variation in Some Wood Properties of Shortleaf Pine in Southeastern Oklahoma

Forest Resource

Testing the occurrence of random mating in polymix pollinations of loblolly pine (Pinus taeda L.)

Typescript (photocopy).Controlled pollinations were performed on five clones with a nine-pollen polymix and each pollen constituent of the polymix. One clone aborted all of its controlled-pollination cones and was excluded from the study. Electrophoretic techniques were used to obtain frequency data on 15 polymorphic isozyme loci. For each cross, chi-square values were calculated on each isozyme locus using the isozyme frequencies in the pollens as the expected. The chi-square analyses found significant variation in three of the remaining four polymix crosses and in four of the 40 individual pollen crosses. Additional analyses on the actual gametic contribution of each pollen in a polymix cross revealed that one of the significant polymix chi-squares was not due to over contribution of gametes by any one or group of pollens, but was instead due to an over contribution of gametes expressing a certain isozyme. An attempt to determine if selection was taking place for any particular pollen parent proved inconclusive. While none of the possible vectors of selection that were measured indicated that selection was indeed occurring, many other possible alternative causes for selection remained untested. If the significant chi-square results are taken literally, then whether the cause of this non-randomness is due to selection or chance, the outcome casts doubt on the general combining abilities and other statistics produced from polymix progeny test data. The chi-square analyses used in this study were sensitive to deviations from the expected due to the small sample size. Pollen parents that over-contributed three to four gametes to the polymix offspring caused significant variation, as measured by the chi-square test, resulting in the rejection of the random mating hypothesis. It must be noted, that no one pollen ever dominated a polymix with 11-45 contributing 20% of the gametes in the BA1-2 polymix being the case of the largest over contribution observed. While the deviations from random mating observed in the BA1-2, S2PT12, and BA3F10-20 polymixes are statistically significant they may not have a measurable effect on the results of field test of polymix crosses

Efficiency tests of a New York air pump

HolographThesis (B.M.E.)--Purdue University, 1895B.M.E

Testing the occurrence of random mating in polymix pollinations of loblolly pine (Pinus taeda L.)

Typescript (photocopy).Controlled pollinations were performed on five clones with a nine-pollen polymix and each pollen constituent of the polymix. One clone aborted all of its controlled-pollination cones and was excluded from the study. Electrophoretic techniques were used to obtain frequency data on 15 polymorphic isozyme loci. For each cross, chi-square values were calculated on each isozyme locus using the isozyme frequencies in the pollens as the expected. The chi-square analyses found significant variation in three of the remaining four polymix crosses and in four of the 40 individual pollen crosses. Additional analyses on the actual gametic contribution of each pollen in a polymix cross revealed that one of the significant polymix chi-squares was not due to over contribution of gametes by any one or group of pollens, but was instead due to an over contribution of gametes expressing a certain isozyme. An attempt to determine if selection was taking place for any particular pollen parent proved inconclusive. While none of the possible vectors of selection that were measured indicated that selection was indeed occurring, many other possible alternative causes for selection remained untested. If the significant chi-square results are taken literally, then whether the cause of this non-randomness is due to selection or chance, the outcome casts doubt on the general combining abilities and other statistics produced from polymix progeny test data. The chi-square analyses used in this study were sensitive to deviations from the expected due to the small sample size. Pollen parents that over-contributed three to four gametes to the polymix offspring caused significant variation, as measured by the chi-square test, resulting in the rejection of the random mating hypothesis. It must be noted, that no one pollen ever dominated a polymix with 11-45 contributing 20% of the gametes in the BA1-2 polymix being the case of the largest over contribution observed. While the deviations from random mating observed in the BA1-2, S2PT12, and BA3F10-20 polymixes are statistically significant they may not have a measurable effect on the results of field test of polymix crosses

Biomass Commercialization Prospects the Next 2 to 5 Years; BIOMASS COLLOQUIES 2000

A series of four colloquies held in the first quarter of 2000 examined the expected development of biomass commercialization in the next 2 to 5 years. Each colloquy included seven to ten representatives from key industries that can contribute to biomass commercialization and who are in positions to influence the future direction. They represented: Corn Growers, Biomass Suppliers, Plant Science Companies, Process Engineering Companies, Chemical Processors, Agri-pulp Suppliers, Current Ethanol Producers, Agricultural Machinery Manufacturers, and Enzyme Suppliers. Others attending included representatives from the National Renewable Energy Lab., Oak Ridge National Laboratory, the U.S. Department of Energy's Office of Fuels Development, the U.S. Department of Agriculture, environmental groups, grower organizations, and members of the financial and economic development community. The informal discussions resulted in improved awareness of the current state, future possibilit ies, and actions that can accelerate commercialization. Biomass commercialization on a large scale has four common issues: (1) Feedstock availability from growers; (2) Large-scale collection and storage; (3) An economic process; (4) Market demand for the product