4 research outputs found
Potentially Pathogenic Airway Bacteria and Neutrophilic Inflammation in Treatment Resistant Severe Asthma
BACKGROUND:Molecular microbiological analysis of airway samples in asthma has demonstrated an altered microbiome in comparison to healthy controls. Such changes may have relevance to treatment-resistant severe asthma, particularly those with neutrophilic airway inflammation, as bacteria might be anticipated to activate the innate immune response, a process that is poorly steroid responsive. An understanding of the relationship between airway bacterial presence and dominance in severe asthma may help direct alternative treatment approaches. OBJECTIVE:We aimed to use a culture independent analysis strategy to describe the presence, dominance and abundance of bacterial taxa in induced sputum from treatment resistant severe asthmatics and correlate findings with clinical characteristics and airway inflammatory markers. METHODS:Induced sputum was obtained from 28 stable treatment-resistant severe asthmatics. The samples were divided for supernatant IL-8 measurement, cytospin preparation for differential cell count and Terminal Restriction Fragment Length Polymorphism (T-RFLP) profiling for bacterial community analysis. RESULTS:In 17/28 patients, the dominant species within the airway bacterial community was Moraxella catarrhalis or a member of the Haemophilus or Streptococcus genera. Colonisation with these species was associated with longer asthma disease duration (mean (SD) 31.8 years (16.7) vs 15.6 years (8.0), p = 0.008), worse post-bronchodilator percent predicted FEV1 (68.0% (24.0) vs 85.5% (19.7), p = 0.025) and higher sputum neutrophil differential cell counts (median (IQR) 80% (67-83) vs 43% (29-67), p = 0.001). Total abundance of these organisms significantly and positively correlated with sputum IL-8 concentration and neutrophil count. CONCLUSIONS:Airway colonisation with potentially pathogenic micro-organisms in asthma is associated with more severe airways obstruction and neutrophilic airway inflammation. This altered colonisation may have a role in the development of an asthma phenotype that responds less well to current asthma therapies
Severe asthma disease duration and inflammation related to abundance of <i>Haemophilus., Streptococcus</i>, and <i>Moraxella sp.</i>
<p>The relationship in treatment-resistant severe asthma between total abundance of <i>Haemophilus sp., Streptococcus sp.</i>, and <i>Moraxella catarrhalis</i> in induced sputum samples and [A] neutrophil differential cell count (%), [B] asthma duration (years), [C] interleukin (IL)-8 concentration in induced sputum and [D] the relationship between <i>M. catarrhalis</i> abundance and induced sputum IL-8 concentration.</p
Interleukin-8 sputum supernatant concentrations in severe asthma related to sputum neutrophil count and lung function.
<p>The relationship in treatment-resistant severe asthma between IL-8 concentrations in induced sputum and (A) neutrophil differential cell counts and (B) Post bronchodilator percent predicted FEV<sub>1</sub>.</p