Histological images of localized HPV analysis by laser-capture micro-dissection (LCM) on a whole tissue section (WTS) positive for HPV16 (Eu 131G), 18 and 39.

<p>Local excision by LCM was performed on colored regions of different grades of cervical intraepithelial neoplasia (CIN), i.e., 1CV and 1CVI from the p16-stained section (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080382#pone-0080382-g003" target="_blank">Figure 3A</a>), and 1BI, 1BII (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080382#pone-0080382-g003" target="_blank">Figure 3B</a>), 1AI, 1AII, and 1AIII (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080382#pone-0080382-g003" target="_blank">Figure 3C</a>) from the hematoxylin and eosin (H&E)-stained section. Excised regions were separately analyzed by LiPA25 and subsequently by the HPV16 variant RHA if HPV16-positive. All Images have been captured by ScanScope XT digital scanner (Aperio Technologies Inc, Vista, Ca, USA). * Region 1BII was positive by LiPA25 for HPV16 but negative by the HPV16 variant RHA.</p

The distribution of HPV16 variants in the swabs of 47 women in the placebo group.

<p>The distribution of HPV16 variants in the swabs of 47 women in the placebo group.</p

Flowchart of selection and subsequent analysis of clinical materials for presence of HPV16 variants.

<p>* This study included 5,144 cervical swab samples from 548 women assigned to the placebo group of a HPV16/18 vaccine trial, which is a subset of the total amount of clinical materials tested during this trial.</p

Definitions of duration of type-specific hr-HPV detection.

<p>*only women positive for hr-HPV at sample month 12 are included in the 2^nd study year and in the analysis of HPV persistency.</p

Woman's risk of having a persistent type-specific hr-HPV type in the 2^nd study year.

<p>SD: standard deviation.</p><p>*Number  =  based on available data, as not all 231 women completely filled-in the questionnaire.</p><p>∧Sexual age  =  years of sexual activity (current age minus age of first sexual intercourse).</p>#<p>Multiple infections  =  irrespective whether these infections were only hr-HPV or also lr-HPV.</p

Influence of the duration of type-specific hr-HPV detection on persistence in 2^nd study year.

<p>Influence of the duration of type-specific hr-HPV detection on persistence in 2^nd study year.</p

A. Prevalence of Low-Risk and High-Risk Types by Age (n = 2065).

<p>Prevalence of overall and both hr- and lr-HPV types showed an almost similar age-distribution. In some women both low-risk and high-risk types were detected. B. Prevalence of Low-Risk and High-Risk Types by Sexual Age (n = 1943). Only sexually active women were selected (n = 1943). Overall HPV prevalence, as well as hr- and lr-HPV prevalence, showed an increase with rising sexual age. However, hr-HPV prevalence decreased from a sexual age of 10 years. In some women both low-risk and high-risk types were detected.</p

HPV prevalence and Odds Ratio's for HPV prevalence among sexually active women using univariate analysis and logistic regression.

<p>HPV+ if one or more genotypes are detected simultaneously.</p><p>Sample sizes change because of missing values of the questionnaire.</p><p>n = number.</p><p>95% C.I. Confidence Interval.</p><p>p: p-value.</p>∧<p>Mann Whitney.</p>*<p>by Chi-square test.</p><p>Ref: reference.</p><p>OCC oral contraceptives.</p>¤<p>Living apart together.</p>**<p>below the age of 10 years several cases of sexual abuse were reported.</p>°<p>STI, Sexually Transmitted Infection.</p>□<p>Sexual age in years with 0 and 1 combined as well as sexual age higher than 13.</p

95% Confidence Interval of HPV Prevalence by Age (n = 2065).

<p>There was an overall increase in HPV prevalence with age till 22 years, afterwards a plateau phase was reached. A decrease is shown at 23 years and a peak among women aged 27 years, however, as the 95% confidence interval is overlapping with adjacent age groups, the differences were considered accidental findings.</p

HPV prevalence by demographic variables among all women.

<p>HPV+ if one or more genotypes(high risk as well as low risk) are detected simultaneously.</p><p>Sample sizes change because of missing values of the questionnaire.</p><p>n = number.</p><p>- not applicable.</p>*<p>by Chi-square test.</p>○<p>ethnicity was self-reported.</p>∧<p>type of education: group of lower secondary education includes 2 women who reported only primary/no education.</p>¤<p>Living apart together.</p><p>OCC oral contraceptives.</p