Kinetics of plasma cell‐free DNA and creatine kinase in a canine model of tissue injury

Background: Cell‐free DNA (cfDNA) comprises short, double‐stranded circulating DNA sequences released from damaged cells. In people, cfDNA concentrations correlate well with disease severity and tissue damage. No reports are available regarding cfDNA kinetics in dogs. Objectives/Hypothesis: Cell‐free DNA will have a short biological half‐life and would be able to stratify mild, moderate, and severe tissue injury. Our study aims were to determine the kinetics and biological half‐life of cfDNA and to contrast them with those of creatine kinase (CK). Animals: Three groups of 10 dogs undergoing open ovariohysterectomy, surgery for cranial cruciate ligament rupture (CCLR), or hemilaminectomy. Methods: Plasma for cfDNA and CK analysis was collected at admission, at induction of anesthesia, postsurgery (time 0) and at 6, 12, 24, 36, 48, 60, and 72 hours after surgery. Results: The biological half‐life of plasma cfDNA and CK were 5.64 hours (95% confidence interval [CI 95], 4.36–7.98 hours) and 28.7 hours (CI95, 25.3–33.3 hours), respectively. In the hemilaminectomy group, cfDNA concentrations differed significantly from admission at 6–12 hours after surgery. Creatine kinase activity differed among the surgical groups and reached a peak 6 hours after surgery. In the ovariohysterectomy and CCLR groups, plasma CK activity 72 hours after surgery did not differ from admission activity of the ovariohysterectomy group. In contrast, in the hemilaminectomy group, plasma CK activity after 72 hours did not return to the ovariohysterectomy group admission activity. Conclusions and Clinical Importance: Plasma CK activity has a longer biological half‐life than previously thought. In contrast to plasma CK activity, cfDNA has a short half‐life and could be a useful marker for peracute severe tissue injury

Stabilization of Hydrodynamic Flows by Small Viscosity Variations

Motivated by the large effect of turbulent drag reduction by minute concentrations of polymers we study the effects of a weakly space-dependent viscosity on the stability of hydrodynamic flows. In a recent Letter [Phys. Rev. Lett. {\bf 87}, 174501, (2001)] we exposed the crucial role played by a localized region where the energy of fluctuations is produced by interactions with the mean flow (the "critical layer"). We showed that a layer of weakly space-dependent viscosity placed near the critical layer can have a very large stabilizing effect on hydrodynamic fluctuations, retarding significantly the onset of turbulence. In this paper we extend these observation in two directions: first we show that the strong stabilization of the primary instability is also obtained when the viscosity profile is realistic (inferred from simulations of turbulent flows with a small concentration of polymers). Second, we analyze the secondary instability (around the time-dependent primary instability) and find similar strong stabilization. Since the secondary instability develops around a time-dependent solution and is three-dimensional, this brings us closer to the turbulent case. We reiterate that the large effect is {\em not} due to a modified dissipation (as is assumed in some theories of drag reduction), but due to reduced energy intake from the mean flow to the fluctuations. We propose that similar physics act in turbulent drag reduction.Comment: 10 pages, 17 figs., REVTeX4, PRE, submitte

The Amundsen Sea Polynya International Research Expedition (ASPIRE)

In search of an explanation for some of the greenest waters ever seen in coastal Antarctica and their possible link to some of the fastest melting glaciers and declining summer sea ice, the Amundsen Sea Polynya International Research Expedition (ASPIRE) challenged the capabilities of the US Antarctic Program and RVIB Nathaniel B. Palmer during Austral summer 2010–2011. We were well rewarded by both an extraordinary research platform and a truly remarkable oceanic setting. Here we provide further insights into the key questions that motivated our sampling approach during ASPIRE and present some preliminary findings, while highlighting the value of the Palmer for accomplishing complex, multifaceted oceanographic research in such a challenging environment

THE CHANGING ECONOMIC SPATIAL STRUCTURE OF EUROPE

Many theoretical and practical works aim at describing the spatial structure of Europe, where spatial relations have undergone continuous change. This article gives an overview of models describing the spatial structure of Europe. Their diversity is highlighted by listing of these models, without any claim to completeness. Our study aims at describing the economic spatial structure of Europe with bi-dimensional regression analysis based on the gravitational model. With the help of the gravity model, we get a spatial image of the spatial structure of Europe. With these images, we can justify the appropriateness of the models based on different methodological backgrounds by comparing them with our results. Our goal is not to create and show a new model that overwrites the existing ones, but rather to contribute to understanding the European spatial structure through a new methodological approach

Dietary fibre to reduce colon cancer risk in Alaska Native people: the Alaska FIRST randomised clinical trial protocol

Introduction Diet, shown to impact colorectal cancer (CRC) risk, is a modifiable environmental factor. Fibre foods fermented by gut microbiota produce metabolites that not only provide food for the colonic epithelium but also exert regulatory effects on colonic mucosal inflammation and proliferation. We describe methods used in a double-blinded, randomised, controlled trial with Alaska Native (AN) people to determine if dietary fibre supplementation can substantially reduce CRC risk among people with the highest reported CRC incidence worldwide. Methods and analyses Eligible patients undergoing routine screening colonoscopy consent to baseline assessments and specimen/data collection (blood, urine, stool, saliva, breath and colon mucosal biopsies) at the time of colonoscopy. Following an 8-week stabilisation period to re-establish normal gut microbiota post colonoscopy, study personnel randomise participants to either a high fibre supplement (resistant starch, n=30) or placebo (digestible starch, n=30) condition, repeating stool sample collection. During the 28-day supplement trial, each participant consumes their usual diet plus their supplement under direct observation. On day 29, participants undergo a flexible sigmoidoscopy to obtain mucosal biopsy samples to measure the effect of the supplement on inflammatory and proliferative biomarkers of cancer risk, with follow-up assessments and data/specimen collection similar to baseline. Secondary outcome measures include the impact of a high fibre supplement on the oral and colonic microbiome and biofluid metabolome. Ethics and dissemination Approvals were obtained from the Alaska Area and University of Pittsburgh Institutional Review Boards and Alaska Native Tribal Health Consortium and Southcentral Foundation research review bodies. A data safety monitoring board, material transfer agreements and weekly study team meetings provide regular oversight throughout the study. Study findings will first be shared with AN tribal leaders, health administrators, providers and community members. Peer-reviewed journal articles and conference presentations will be forthcoming once approved by tribal review bodies

The phase relation between sunspot numbers and soft X-ray flares

To better understand long-term flare activity, we present a statistical study on soft X-ray flares from May 1976 to May 2008. It is found that the smoothed monthly peak fluxes of C-class, M-class, and X-class flares have a very noticeable time lag of 13, 8, and 8 months in cycle 21 respectively with respect to the smoothed monthly sunspot numbers. There is no time lag between the sunspot numbers and M-class flares in cycle 22. However, there is a one-month time lag for C-class flares and a one-month time lead for X-class flares with regard to sunspot numbers in cycle 22. For cycle 23, the smoothed monthly peak fluxes of C-class, M-class, and X-class flares have a very noticeable time lag of one month, 5 months, and 21 months respectively with respect to sunspot numbers. If we take the three types of flares together, the smoothed monthly peak fluxes of soft X-ray flares have a time lag of 9 months in cycle 21, no time lag in cycle 22 and a characteristic time lag of 5 months in cycle 23 with respect to the smoothed monthly sunspot numbers. Furthermore, the correlation coefficients of the smoothed monthly peak fluxes of M-class and X-class flares and the smoothed monthly sunspot numbers are higher in cycle 22 than those in cycles 21 and 23. The correlation coefficients between the three kinds of soft X-ray flares in cycle 22 are higher than those in cycles 21 and 23. These findings may be instructive in predicting C-class, M-class, and X-class flares regarding sunspot numbers in the next cycle and the physical processes of energy storage and dissipation in the corona.Comment: 8 pages, 3 figures, Accepted for publication in Astrophysics & Space Scienc

Validity of physical activity monitors during daily life in patients with COPD.

Symptoms during physical activity (PA) and physical inactivity are COPD. Our aim was to evaluate the validity and usability of six activity in patients with COPD against the doubly labelled water (DLW) indirect calorimetry method.Eighty COPD patients (age 68+/-6 years, FEV1 57+/-19% predicted) recruited in four centres each wore simultaneously three or six commercially available monitors validated in chronic conditions for consecutive days. A priori validity criteria were defined. These ability to explain total energy expenditure (TEE) variance through regression analysis, using TEE as the dependent variable with total body (TBW) plus several PA monitors outputs as independent variables; and with DLW measured activity energy expenditure (AEE).The Actigraph GT3X DynaPort MoveMonitor best explained the majority of the TEE variance not explained by TBW (53% and 70% respectively) and showed the most correlations with AEE (r=0.71 p<0.001, r=0.70 p<0.0001, of this study should guide users in choosing valid activity monitors for or for clinical use in patients with chronic diseases such as COPD

A 750 mW, continuous-wave, solid-state laser source at 313 nm for cooling and manipulating trapped 9Be+ ions

We present a solid-state laser system that generates 750 mW of continuous-wave single-frequency output at 313 nm. Sum-frequency generation with fiber lasers at 1550 nm and 1051 nm produces up to 2 W at 626 nm. This visible light is then converted to UV by cavity-enhanced second-harmonic generation. The laser output can be tuned over a 495 GHz range, which includes the 9Be+ laser cooling and repumping transitions. This is the first report of a narrow-linewidth laser system with sufficient power to perform fault-tolerant quantum-gate operations with trapped 9Be+ ions by use of stimulated Raman transitions.Comment: 9 pages, 4 figure

Gemini Observations of Disks and Jets in Young Stellar Objects and in Active Galaxies

We present first results from the Near-infrared Integral Field Spectrograph (NIFS) located at Gemini North. For the active galaxies Cygnus A and Perseus A we observe rotationally-supported accretion disks and adduce the existence of massive central black holes and estimate their masses. In Cygnus A we also see remarkable high-excitation ionization cones dominated by photoionization from the central engine. In the T-Tauri stars HV Tau C and DG Tau we see highly-collimated bipolar outflows in the [Fe II] 1.644 micron line, surrounded by a slower molecular bipolar outflow seen in the H_2 lines, in accordance with the model advocated by Pyo et al. (2002).Comment: Invited paper presented at the 5th Stromlo Symposium. 9 pages, 7 figures. Accepted for publication in Astrophysics & Space Scienc

Reuse of Treated Wastewater: Drivers, Regulations, Technologies, Case Studies, and Greater Chicago Area Experiences

Water reuse is a practical solution to augment water supplies in areas where water resources are increasingly scarce. Water reuse technology is versatile and can be used to alleviate the different causes of water scarcity, such as groundwater depletion or increased availability of brackish water. Treatment technologies can be tailored specifically to the end use of recycled water, focusing on these drivers that are region-specific, for a more cost-effective treatment system. This is called a “Fit-for-Purpose” strategy that is commonly implemented in any water reuse project. However, implementing water reuse can be challenging due to infrastructural requirements, economic issues, and social acceptance. To help navigate these challenges, this article provides a comprehensive review of water reuse cases and presents guidelines that can act as a reference framework for future water reuse projects. This article also makes the case for implementing water reuse in the Greater Chicago area as a means of alleviating pressure on withdrawals from Lake Michigan