Overview of the cloning efficiency.

<p>Overview of the cloning efficiency.</p

Identification of <i>MlJAZ1</i>.

<p>(A) cDNA-AFLP gel. (B) Detail of the cDNA-AFLP gel showing the MeJA response of the ML074 tag. (C) Result of the PCR screening, showing the presence of ML074 in pools 1, 6, 7, 8, and 12. (D) Schematic representation of <i>MlJAZ1</i>. (E) Autoradiogram of pool 7 after hybridization with the radioactively labeled ML074 tag; arrows mark the three candidate colonies. (F) Colony PCR on the candidate colonies. The cDNA-AFLP tag was used as template for the positive control. (G) Identification of the tify and Jas domains in the MlJAZ1 amino acid sequence.</p