A magnetic resonance multi-atlas for the neonatal rabbit brain - Dataset

<p>Dataset related to the Neuroimage paper https://doi.org/10.1016/j.neuroimage.2018.06.029.  Download links, documentation and code for the manipulation are available from the software repository https://github.com/gift-surg/SPOT-A-NeonatalRabbit</p

Figure 5 raw data. The raw values for each shear metric within the instrumented and control arteries of each mouse from Influence of shear stress magnitude and direction on atherosclerotic plaque composition

The precise flow characteristics that promote different atherosclerotic plaque types remain unclear. We previously developed a blood flow-modifying cuff for ApoE^−/− mice that induces the development of advanced plaques with vulnerable and stable features upstream and downstream of the cuff, respectively. Herein, we sought to test the hypothesis that changes in flow magnitude promote formation of the upstream (vulnerable) plaque, whereas altered flow direction is important for development of the downstream (stable) plaque. We instrumented ApoE^−/− mice (<i>n</i> = 7) with a cuff around the left carotid artery and imaged them with micro-CT (39.6 µm resolution) eight to nine weeks after cuff placement. Computational fluid dynamics was then performed to compute six metrics that describe different aspects of atherogenic flow in terms of wall shear stress magnitude and/or direction. In a subset of four imaged animals, we performed histology to confirm the presence of advanced plaques and measure plaque length in each segment. Relative to the control artery, the region upstream of the cuff exhibited changes in shear stress magnitude only (<i>p</i> < 0.05), whereas the region downstream of the cuff exhibited changes in shear stress magnitude and direction (<i>p</i> < 0.05). These data suggest that shear stress magnitude contributes to the formation of advanced plaques with a vulnerable phenotype, whereas variations in both magnitude and direction promote the formation of plaques with stable features

Effect of pre-treatment with AMPH on DAT and DRD1 binding (autoradiography).

<p>Effect of pre-treatment with AMPH on DAT and DRD1 binding (autoradiography).</p

Whole brain analyses of phMRI data.

<p>The three rows show 1 mm-thick coronal slices for different group comparisons: a) MPH challenge in saline-treated rats increased BOLD response compared to groups that received a saline challenge b) MPH challenge in AMPH-pre-treated rats increased the BOLD response extensively c) AMPH-pre-treated rats show increased BOLD response compared to the SALMPH group. Images are thresholded at Z = 1.6.</p

ROI analyses of phMRI data.

<p>phMRI time courses in the CPu (a) and NAcc (b). MPH or saline challenge was administered after 12 volumes (indicated by the arrow) followed by 38 volumes post-administration. The AMPH-MPH group differed significantly from the SAL-MPH and SAL groups. Difference between pre-injection (1–12) and post-injection (15–35) displayed for the CPU and Nacc (c) for each group (mean ±SEM)* p<0.05.</p

Immunocytochemistry.

<p>a) GFAP b) DRD1 c) DAT and d) DRD2 expression following AMPH or saline pre-treatment in the NAcc and CPu (mean ±SEM) * p<0.05.</p

Effect of pre-treatment with AMPH on brain monoamines and metabolites.

<p>Effect of pre-treatment with AMPH on brain monoamines and metabolites.</p