Distributional Impacts of Retail Vaccine Availability

We examine the potential for exploiting retailer location choice in targeting health interventions. Using geospatial data, we quantify proximity to vaccines created by a U.S. federal program distributing COVID-19 vaccines to commercial retail pharmacies. We assess the distributional impacts of a proposal to provide vaccines at Dollar General, a low-priced general merchandise retailer. Adding Dollar General to the federal program would substantially decrease the distance to vaccine sites for low-income, rural, and minority U.S. households, groups for which COVID-19 vaccine take-up has been disproportionately slow

A Novel Method for Reducing the Effect of Tonic Muscle Activity on the Gamma Band of the Scalp EEG

Neural oscillations in the gamma band are of increasing interest, but separating them from myogenic electrical activity has proved difficult. A novel algorithm has been developed to reduce the effect of tonic scalp and neck muscle activity on the gamma band of the EEG. This uses mathematical modelling to fit individual muscle spikes and then subtracts them from the data. The method was applied to the detection of motor associated gamma in two separate groups of eight subjects using different sampling rates. A reproducible increase in high gamma (65–85 Hz) magnitude occurred immediately after the motor action in the left central area (p = 0.02 and p = 0.0002 for the two cohorts with individually optimized algorithm parameters, compared to p = 0.03 and p = 0.16 before correction). Whilst the magnitude of this event-related gamma synchronisation was not reduced by the application of the EMG reduction algorithm, the baseline left central gamma magnitude was significantly reduced by an average of 23 % with a faster sampling rate (p < 0.05). In comparison, at left and right temporo-parietal locations the gamma amplitude was reduced by 60 and 54 % respectively (p < 0.05). The reduction of EMG contamination by fitting and subtraction of individual spikes shows promise as a method of improving the signal to noise ratio of high frequency neural oscillations in scalp EEG

Family Members with Overlapping Mental Health Needs Require the Transformation of Systems and Services

Women and men with a lifetime prevalence of psychiatric disorder are at least as likely to be parents as are adults without psychiatric disorder. The majority of adults in all diagnostic categories are parents, including those meeting criteria for affective and anxiety disorders, PTSD, and non-affective psychosis. Children with Serious Emotional Disturbance (SED) receiving services in Systems of Care (SOCs) programs may have multiple family risk factors. Family-centered, strengths-based practices require a paradigm shift in the way administrators and providers view and intervene with children and adults. Presented at The Santa Fe Summit on Behavioral Health, the American College of Mental Health Administration, Santa Fe, New Mexico, March 2005

Use of serum lactate levels to predict survival for patients with out-of-hospital cardiac arrest: A cohort study

Objectives: We examined the association of serum lactate levels and early lactate clearance with survival to hospital discharge for patients suffering an out-of-hospital cardiac arrest (OHCA). Methods: A retrospective cohort analysis was performed of patients with OHCA transported by ambulance to two adult tertiary hospitals in Perth, Western Australia. Exclusion criteria were traumatic cardiac arrest, return of spontaneous circulation prior to the arrival of the ambulance, age less than 18 years and no serum lactate levels recorded. Serum lactate levels recorded for up to 48h post-arrest were obtained from the hospital clinical information system, and lactate clearance over 48h was calculated. Descriptive and logistic regression analyses were conducted. Results: There were 518 patients with lactate values, of whom 126 (24.3%) survived to hospital discharge. Survivors and non-survivors had different mean initial lactate levels (mean±SD 6.9±4.7 and 12.2±5.5mmol/L, respectively; P&lt;0.001). Lactate clearance was higher in survivors. Lactate levels for non-survivors did not decrease below 2mmol/L until at least 30h after the ambulance call. Conclusion: In OHCA patients who had serum lactate levels measured, both lower initial serum lactate and early lactate clearance in the first 48h following OHCA were associated with increased likelihood of survival. However, the use of lactate in isolation as a predictor of survival or neurological outcome is not recommended. Prospective studies that minimise selection bias are required to determine the clinical utility of serum lactate levels in OHCA patients. © 2016 Australasian College for Emergency Medicine and Australasian Society for Emergency Medicine

Molecular mechanism for kinesin-1 direct membrane recognition

The cargo-binding capabilities of cytoskeletal motor proteins have expanded during evolution through both gene duplication and alternative splicing. For the light chains of the kinesin-1 family of microtubule motors, this has resulted in an array of carboxyl-terminal domain sequences of unknown molecular function. Here, combining phylogenetic analyses with biophysical, biochemical, and cell biology approaches, we identify a highly conserved membrane-induced curvature-sensitive amphipathic helix within this region of a subset of long kinesin light-chain paralogs and splice isoforms. This helix mediates the direct binding of kinesin-1 to lipid membranes. Membrane binding requires specific anionic phospholipids, and it contributes to kinesin-1\u2013dependent lysosome positioning, a canonical activity that, until now, has been attributed exclusively the recognition of organelle-associated cargo adaptor proteins. This leads us to propose a protein-lipid coincidence detection framework for kinesin-1\u2013mediated organelle transport

Single base mutations in the nucleocapsid gene of SARS-CoV-2 affects amplification efficiency of sequence variants and may lead to assay failure

Reverse transcriptase quantitative PCR (RT-qPCR) is the main diagnostic assay used to detect SARS-CoV-2 RNA in respiratory samples. RT-qPCR is performed by specifically targeting the viral genome using complementary oligonucleotides called primers and probes. This approach relies on prior knowledge of the genetic sequence of the target. Viral genetic variants with changes to the primer/probe binding region may reduce the performance of PCR assays and have the potential to cause assay failure. In this work we demonstrate how two single nucleotide variants (SNVs) altered the amplification curve of a diagnostic PCR targeting the Nucleocapsid (N) gene and illustrate how threshold setting can lead to false-negative results even where the variant sequence is amplified. We also describe how in silico analysis of SARS-CoV-2 genome sequences available in the COVID-19 Genomics UK Consortium (COG-UK) and GISAID databases was performed to predict the impact of sequence variation on the performance of 22 published PCR assays. The vast majority of published primer and probe sequences contain sequence mismatches with at least one SARS-CoV-2 lineage. We recommend that visual observation of amplification curves is included as part of laboratory quality procedures, even in high throughput settings where thresholds are set automatically and that in silico analysis is used to monitor the potential impact of new variants on established assays. Ideally comprehensive in silico analysis should be applied to guide selection of highly conserved genomic regions to target with future SARS-CoV-2 PCR assays

Symbiotic starburst-black hole AGN -- I. Isothermal hydrodynamics of the mass-loaded ISM

Compelling evidence associates the nuclei of active galaxies and massive starbursts. The symbiosis between a compact nuclear starburst stellar cluster and a massive black hole can self-consistently explain the properties of active nuclei. The young stellar cluster has a profound effect on the most important observable properties of active galaxies through its gravity, and by mass injection through stellar winds, supernovae and stellar collisions. Mass injection generates a nuclear ISM which flows under gravitational and radiative forces until it leaves the nucleus or is accreted onto the black hole or accretion disc. The radiative force exerted by the black hole--accretion disc radiation field is not spherically symmetric. This results in complex flows in which regions of inflow can coexist with high Mach number outflowing winds and hydrodynamic jets. We present two-dimensional hydrodynamic models of such nISM flows, which are highly complex and time variable. Shocked shells, jets and explosive bubbles are produced, with bipolar winds driving out from the nucleus. Our results graphically illustrate why broad emission line studies have consistently failed to identify any simple, global flow geometry. The real structure of the flows is _inevitably_ yet more complex.Comment: 51 pages, 85 postscript figures, Latex, using MNRAS macros, to be published in MNRAS. Postscript will full resolution pictures and mpeg simulations available via http://ast.leeds.ac.uk/~rjrw/agn.htm

Islands of linkage in an ocean of pervasive recombination reveals two-speed evolution of human cytomegalovirus genomes

Human cytomegalovirus (HCMV) infects most of the population worldwide, persisting throughout the host's life in a latent state with periodic episodes of reactivation. While typically asymptomatic, HCMV can cause fatal disease among congenitally infected infants and immunocompromised patients. These clinical issues are compounded by the emergence of antiviral resistance and the absence of an effective vaccine, the development of which is likely complicated by the numerous immune evasins encoded by HCMV to counter the host's adaptive immune responses, a feature that facilitates frequent super-infections. Understanding the evolutionary dynamics of HCMV is essential for the development of effective new drugs and vaccines. By comparing viral genomes from uncultivated or low-passaged clinical samples of diverse origins, we observe evidence of frequent homologous recombination events, both recent and ancient, and no structure of HCMV genetic diversity at the whole-genome scale. Analysis of individual gene-scale loci reveals a striking dichotomy: while most of the genome is highly conserved, recombines essentially freely and has evolved under purifying selection, 21 genes display extreme diversity, structured into distinct genotypes that do not recombine with each other. Most of these hyper-variable genes encode glycoproteins involved in cell entry or escape of host immunity. Evidence that half of them have diverged through episodes of intense positive selection suggests that rapid evolution of hyper-variable loci is likely driven by interactions with host immunity. It appears that this process is enabled by recombination unlinking hyper-variable loci from strongly constrained neighboring sites. It is conceivable that viral mechanisms facilitating super-infection have evolved to promote recombination between diverged genotypes, allowing the virus to continuously diversify at key loci to escape immune detection, while maintaining a genome optimally adapted to its asymptomatic infectious lifecycle

The Ursinus Weekly, March 19, 1970

New USGA studies dress code, rights • Campus Chest Committee selects student-faculty cast and chorus • Second Arts Festival considered successful • Senior class plans prom, graduation; Newman contacted • Girls basketball team captures third place in national competition • Editorial: Dress regulations • Focus: Vincent Scancella • Letters to the editor: Freak weekend; Lantern review; Inconsiderate students; Meal mess; Protheater thanks; Open dorm rule; Action line; Interest; Both sides now • Perspectives: The first victory • Dining Hall dishwashers enjoy most fun job • Opinion: A petty organization • Perusing the catalog • Cafeteria meals, open dorms proposed to aid Ursinus budget • Recruiting difficult, claim Bear coaches • Ted Taylor chosen as new coach • Freshman hoopster tops Bear scoring • Matmen end 1-9 campaign with hope for next seasonhttps://digitalcommons.ursinus.edu/weekly/1157/thumbnail.jp